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Promega pblcat2
Pblcat2, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pblcat2/pblcat2/pm18295963-51-24-22
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Article Title: Activation and inhibition of the erythropoietin receptor by a membrane-anchored erythropoietin.
Article Snippet: erythropoietin receptor by a membrane-anchored erythropoietin Olivier Negre, Floriane Fusil, Annie Henri, Jean-Marie Villette, Philippe Leboulch, Yves Beuzard, and Emmanuel Payen INSERM, Laboratoire de Thérapie Génique Hématopoı̈étique, Paris, France; Université Denis Diderot-Paris 7, Institut Universitaire d’Hématologie, Paris, France; AP-HP, Hôpital Saint-Louis, Paris, France; Harvard Medical School and Brigham Women’s Hospital, Cambridge, Mass., USA

Construct:

Article Title: Glucocorticoids Stimulate p21 Gene Expression by Targeting Multiple Transcriptional Elements within a Steroid Responsive Region of the p21 Promoter in Rat Hepatoma Cells
Article Snippet: The chimeric p21 promoter CAT reporter plasmids containing deletions at 22.326 kb, 21.892 kb, 21.481 kb, 21.184 kb, 20.883 kb, 20.585 kb, and 20.291 kb, and the p21-luciferase reporter plasmids were generous gifts from B. Vogelstein (Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore) and have been described previously (41). .. Fine mapping deletion constructs were PCR-amplified from the full-length 22.326p21 CAT reporter plasmid and cloned into pBLCAT2 (Promega). ..

Polymerase Chain Reaction:

Article Title: Glucocorticoids Stimulate p21 Gene Expression by Targeting Multiple Transcriptional Elements within a Steroid Responsive Region of the p21 Promoter in Rat Hepatoma Cells
Article Snippet: The chimeric p21 promoter CAT reporter plasmids containing deletions at 22.326 kb, 21.892 kb, 21.481 kb, 21.184 kb, 20.883 kb, 20.585 kb, and 20.291 kb, and the p21-luciferase reporter plasmids were generous gifts from B. Vogelstein (Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore) and have been described previously (41). .. Fine mapping deletion constructs were PCR-amplified from the full-length 22.326p21 CAT reporter plasmid and cloned into pBLCAT2 (Promega). ..

Article Title: Cloning and characterization of the human protein kinase C-eta promoter.
Article Snippet: .. Silencer and enhancer-like fragments spanning positions 22207 to 21645 and positions 21641 to 21084, respectively, were amplified by PCR and then inserted into the pBLCAT2 and pCAT3 promoter vectors by guest on M arch 6, 2015 http://w w w .jbc.org/ D ow nloaded from (Promega). ..

Plasmid Preparation:

Article Title: Glucocorticoids Stimulate p21 Gene Expression by Targeting Multiple Transcriptional Elements within a Steroid Responsive Region of the p21 Promoter in Rat Hepatoma Cells
Article Snippet: The chimeric p21 promoter CAT reporter plasmids containing deletions at 22.326 kb, 21.892 kb, 21.481 kb, 21.184 kb, 20.883 kb, 20.585 kb, and 20.291 kb, and the p21-luciferase reporter plasmids were generous gifts from B. Vogelstein (Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore) and have been described previously (41). .. Fine mapping deletion constructs were PCR-amplified from the full-length 22.326p21 CAT reporter plasmid and cloned into pBLCAT2 (Promega). ..

Article Title: Identification of a functional androgen-response element in the exon 1-coding sequence of the cystatin-related protein gene crp2.
Article Snippet: Religated plasmids were transfected in XL1-Blue cells (Stratagene, La Jolla, CA), and clones containing the recombinant plasmids, displaying inserts of about 600 bp, were analyzed by sequencing to determine the exact 39-end of the gene insert. .. After SalI/NsiI digestion, the promoter fragments were cloned in the SalI/PstI double-digested vectors pCATEnh vector (Promega) or pBLCAT2 (crp1P: 2584/111). ..

Clone Assay:

Article Title: Glucocorticoids Stimulate p21 Gene Expression by Targeting Multiple Transcriptional Elements within a Steroid Responsive Region of the p21 Promoter in Rat Hepatoma Cells
Article Snippet: The chimeric p21 promoter CAT reporter plasmids containing deletions at 22.326 kb, 21.892 kb, 21.481 kb, 21.184 kb, 20.883 kb, 20.585 kb, and 20.291 kb, and the p21-luciferase reporter plasmids were generous gifts from B. Vogelstein (Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore) and have been described previously (41). .. Fine mapping deletion constructs were PCR-amplified from the full-length 22.326p21 CAT reporter plasmid and cloned into pBLCAT2 (Promega). ..

Article Title: Identification of a functional androgen-response element in the exon 1-coding sequence of the cystatin-related protein gene crp2.
Article Snippet: Religated plasmids were transfected in XL1-Blue cells (Stratagene, La Jolla, CA), and clones containing the recombinant plasmids, displaying inserts of about 600 bp, were analyzed by sequencing to determine the exact 39-end of the gene insert. .. After SalI/NsiI digestion, the promoter fragments were cloned in the SalI/PstI double-digested vectors pCATEnh vector (Promega) or pBLCAT2 (crp1P: 2584/111). ..

Amplification:

Article Title: Cloning and characterization of the human protein kinase C-eta promoter.
Article Snippet: .. Silencer and enhancer-like fragments spanning positions 22207 to 21645 and positions 21641 to 21084, respectively, were amplified by PCR and then inserted into the pBLCAT2 and pCAT3 promoter vectors by guest on M arch 6, 2015 http://w w w .jbc.org/ D ow nloaded from (Promega). ..



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FIG. 8. Evaluation of pTRE no. 5 in the context of a heterologous promoter. A, Ac- tivities of two copies of a PAL TRE lucif- erase reporter construct in response to 100 nM T3 in UMR106 osteoblasts in the ab- sence and presence of TR1. B, Relative activities of pTRE5-TKpBLCAT2 reporter construct in response to 100 nM T3 or rT3 in UMR106 cells in the absence or presence of TR1. C, Relative activities of pTRE5-TK- <t>pBLCAT2,</t> M1-TKpBLCAT2, and M3-TK- pBLCAT2 reporter constructs in response to 100 nM T3 in UMR106 cells in the ab- sence or presence of TR1. Data are ex- pressed as mean SEM and were analyzed with one-way ANOVA, followed by the Tukey’s multiple comparison test (*, P 0.05; ***, P 0.001; three independent ex- periments performed in triplicate).
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FIG. 8. Evaluation of pTRE no. 5 in the context of a heterologous promoter. A, Ac- tivities of two copies of a PAL TRE lucif- erase reporter construct in response to 100 nM T3 in UMR106 osteoblasts in the ab- sence and presence of TR1. B, Relative activities of pTRE5-TKpBLCAT2 reporter construct in response to 100 nM T3 or rT3 in UMR106 cells in the absence or presence of TR1. C, Relative activities of pTRE5-TK- <t>pBLCAT2,</t> M1-TKpBLCAT2, and M3-TK- pBLCAT2 reporter constructs in response to 100 nM T3 in UMR106 cells in the ab- sence or presence of TR1. Data are ex- pressed as mean SEM and were analyzed with one-way ANOVA, followed by the Tukey’s multiple comparison test (*, P 0.05; ***, P 0.001; three independent ex- periments performed in triplicate).
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FIG. 8. Evaluation of pTRE no. 5 in the context of a heterologous promoter. A, Ac- tivities of two copies of a PAL TRE lucif- erase reporter construct in response to 100 nM T3 in UMR106 osteoblasts in the ab- sence and presence of TR1. B, Relative activities of pTRE5-TKpBLCAT2 reporter construct in response to 100 nM T3 or rT3 in UMR106 cells in the absence or presence of TR1. C, Relative activities of pTRE5-TK- <t>pBLCAT2,</t> M1-TKpBLCAT2, and M3-TK- pBLCAT2 reporter constructs in response to 100 nM T3 in UMR106 cells in the ab- sence or presence of TR1. Data are ex- pressed as mean SEM and were analyzed with one-way ANOVA, followed by the Tukey’s multiple comparison test (*, P 0.05; ***, P 0.001; three independent ex- periments performed in triplicate).
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FIG. 8. Evaluation of pTRE no. 5 in the context of a heterologous promoter. A, Ac- tivities of two copies of a PAL TRE lucif- erase reporter construct in response to 100 nM T3 in UMR106 osteoblasts in the ab- sence and presence of TR1. B, Relative activities of pTRE5-TKpBLCAT2 reporter construct in response to 100 nM T3 or rT3 in UMR106 cells in the absence or presence of TR1. C, Relative activities of pTRE5-TK- <t>pBLCAT2,</t> M1-TKpBLCAT2, and M3-TK- pBLCAT2 reporter constructs in response to 100 nM T3 in UMR106 cells in the ab- sence or presence of TR1. Data are ex- pressed as mean SEM and were analyzed with one-way ANOVA, followed by the Tukey’s multiple comparison test (*, P 0.05; ***, P 0.001; three independent ex- periments performed in triplicate).
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ATCC bamhi site
FIG. 8. Evaluation of pTRE no. 5 in the context of a heterologous promoter. A, Ac- tivities of two copies of a PAL TRE lucif- erase reporter construct in response to 100 nM T3 in UMR106 osteoblasts in the ab- sence and presence of TR1. B, Relative activities of pTRE5-TKpBLCAT2 reporter construct in response to 100 nM T3 or rT3 in UMR106 cells in the absence or presence of TR1. C, Relative activities of pTRE5-TK- <t>pBLCAT2,</t> M1-TKpBLCAT2, and M3-TK- pBLCAT2 reporter constructs in response to 100 nM T3 in UMR106 cells in the ab- sence or presence of TR1. Data are ex- pressed as mean SEM and were analyzed with one-way ANOVA, followed by the Tukey’s multiple comparison test (*, P 0.05; ***, P 0.001; three independent ex- periments performed in triplicate).
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FIG. 8. Evaluation of pTRE no. 5 in the context of a heterologous promoter. A, Ac- tivities of two copies of a PAL TRE lucif- erase reporter construct in response to 100 nM T3 in UMR106 osteoblasts in the ab- sence and presence of TR1. B, Relative activities of pTRE5-TKpBLCAT2 reporter construct in response to 100 nM T3 or rT3 in UMR106 cells in the absence or presence of TR1. C, Relative activities of pTRE5-TK- pBLCAT2, M1-TKpBLCAT2, and M3-TK- pBLCAT2 reporter constructs in response to 100 nM T3 in UMR106 cells in the ab- sence or presence of TR1. Data are ex- pressed as mean SEM and were analyzed with one-way ANOVA, followed by the Tukey’s multiple comparison test (*, P 0.05; ***, P 0.001; three independent ex- periments performed in triplicate).

Journal: Endocrinology

Article Title: Regulation of fibroblast growth factor receptor-1 (FGFR1) by thyroid hormone: identification of a thyroid hormone response element in the murine Fgfr1 promoter.

doi: 10.1210/en.2007-0114

Figure Lengend Snippet: FIG. 8. Evaluation of pTRE no. 5 in the context of a heterologous promoter. A, Ac- tivities of two copies of a PAL TRE lucif- erase reporter construct in response to 100 nM T3 in UMR106 osteoblasts in the ab- sence and presence of TR1. B, Relative activities of pTRE5-TKpBLCAT2 reporter construct in response to 100 nM T3 or rT3 in UMR106 cells in the absence or presence of TR1. C, Relative activities of pTRE5-TK- pBLCAT2, M1-TKpBLCAT2, and M3-TK- pBLCAT2 reporter constructs in response to 100 nM T3 in UMR106 cells in the ab- sence or presence of TR1. Data are ex- pressed as mean SEM and were analyzed with one-way ANOVA, followed by the Tukey’s multiple comparison test (*, P 0.05; ***, P 0.001; three independent ex- periments performed in triplicate).

Article Snippet: In addition, the identified pTRE sequence was cloned, along with mutant TRE sequences, upstream of a heterologous thymidine kinase (TK) promoter, into the pBLCAT2 CAT-reporter plasmid (American Type Culture Collection, Manassas, VA).

Techniques: Construct, Comparison