pblcat2 (Promega)
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Pblcat2, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pblcat2/pblcat2/pm18295963-51-24-22
Average 90 stars, based on 1 article reviews
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other:Article Title: Activation and inhibition of the erythropoietin receptor by a membrane-anchored erythropoietin. Article Snippet: erythropoietin receptor by a membrane-anchored erythropoietin Olivier Negre, Floriane Fusil, Annie Henri, Jean-Marie Villette, Philippe Leboulch, Yves Beuzard, and Emmanuel Payen INSERM, Laboratoire de Thérapie Génique Hématopoı̈étique, Paris, France; Université Denis Diderot-Paris 7, Institut Universitaire d’Hématologie, Paris, France; AP-HP, Hôpital Saint-Louis, Paris, France; Harvard Medical School and Brigham Women’s Hospital, Cambridge, Mass., USA Construct:Article Title: Glucocorticoids Stimulate p21 Gene Expression by Targeting Multiple Transcriptional Elements within a Steroid Responsive Region of the p21 Promoter in Rat Hepatoma Cells Article Snippet: The chimeric p21 promoter CAT reporter plasmids containing deletions at 22.326 kb, 21.892 kb, 21.481 kb, 21.184 kb, 20.883 kb, 20.585 kb, and 20.291 kb, and the p21-luciferase reporter plasmids were generous gifts from B. Vogelstein (Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore) and have been described previously (41). .. Fine mapping deletion constructs were PCR-amplified from the full-length 22.326p21 CAT reporter plasmid and cloned into Polymerase Chain Reaction:Article Title: Glucocorticoids Stimulate p21 Gene Expression by Targeting Multiple Transcriptional Elements within a Steroid Responsive Region of the p21 Promoter in Rat Hepatoma Cells Article Snippet: The chimeric p21 promoter CAT reporter plasmids containing deletions at 22.326 kb, 21.892 kb, 21.481 kb, 21.184 kb, 20.883 kb, 20.585 kb, and 20.291 kb, and the p21-luciferase reporter plasmids were generous gifts from B. Vogelstein (Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore) and have been described previously (41). .. Fine mapping deletion constructs were PCR-amplified from the full-length 22.326p21 CAT reporter plasmid and cloned into Article Title: Cloning and characterization of the human protein kinase C-eta promoter. Article Snippet: .. Silencer and enhancer-like fragments spanning positions 22207 to 21645 and positions 21641 to 21084, respectively, were amplified by PCR and then inserted into the Plasmid Preparation:Article Title: Glucocorticoids Stimulate p21 Gene Expression by Targeting Multiple Transcriptional Elements within a Steroid Responsive Region of the p21 Promoter in Rat Hepatoma Cells Article Snippet: The chimeric p21 promoter CAT reporter plasmids containing deletions at 22.326 kb, 21.892 kb, 21.481 kb, 21.184 kb, 20.883 kb, 20.585 kb, and 20.291 kb, and the p21-luciferase reporter plasmids were generous gifts from B. Vogelstein (Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore) and have been described previously (41). .. Fine mapping deletion constructs were PCR-amplified from the full-length 22.326p21 CAT reporter plasmid and cloned into Article Title: Identification of a functional androgen-response element in the exon 1-coding sequence of the cystatin-related protein gene crp2. Article Snippet: Religated plasmids were transfected in XL1-Blue cells (Stratagene, La Jolla, CA), and clones containing the recombinant plasmids, displaying inserts of about 600 bp, were analyzed by sequencing to determine the exact 39-end of the gene insert. .. After SalI/NsiI digestion, the promoter fragments were cloned in the SalI/PstI double-digested vectors pCATEnh vector (Promega) or Clone Assay:Article Title: Glucocorticoids Stimulate p21 Gene Expression by Targeting Multiple Transcriptional Elements within a Steroid Responsive Region of the p21 Promoter in Rat Hepatoma Cells Article Snippet: The chimeric p21 promoter CAT reporter plasmids containing deletions at 22.326 kb, 21.892 kb, 21.481 kb, 21.184 kb, 20.883 kb, 20.585 kb, and 20.291 kb, and the p21-luciferase reporter plasmids were generous gifts from B. Vogelstein (Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore) and have been described previously (41). .. Fine mapping deletion constructs were PCR-amplified from the full-length 22.326p21 CAT reporter plasmid and cloned into Article Title: Identification of a functional androgen-response element in the exon 1-coding sequence of the cystatin-related protein gene crp2. Article Snippet: Religated plasmids were transfected in XL1-Blue cells (Stratagene, La Jolla, CA), and clones containing the recombinant plasmids, displaying inserts of about 600 bp, were analyzed by sequencing to determine the exact 39-end of the gene insert. .. After SalI/NsiI digestion, the promoter fragments were cloned in the SalI/PstI double-digested vectors pCATEnh vector (Promega) or Amplification:Article Title: Cloning and characterization of the human protein kinase C-eta promoter. Article Snippet: .. Silencer and enhancer-like fragments spanning positions 22207 to 21645 and positions 21641 to 21084, respectively, were amplified by PCR and then inserted into the |
