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Promega pbind-gr
Pbind Gr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbind-gr/pbind+gr/pm39310986-40-7-14
Average 90 stars, based on 1 article reviews
pbind-gr - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Luciferase:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Cell Based Assay:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Activation Assay:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Activity Assay:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Ligand Binding Assay:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Binding Assay:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Sequencing:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Expressing:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Stable Transfection:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Transfection:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Gene Expression:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Reporter Gene Assay:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


Plasmid Preparation:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).


shRNA:

Article Title: PFO5DoDA disrupts hepatic homeostasis primarily through glucocorticoid signaling inhibition.
Article Snippet: • Long-term low-dose PFO5DoDA exposure suppressed cellular stress in male

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport.
Article Snippet: Approximately 1.25x105 PC3 cells and 5.0x105 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions.
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Glucocorticoid Receptor (GR) Co-Activator Luciferase Reporter Cell Based Assay Glucocorticoid Activation Assay The activity of steroid payloads and anti-PRLR steroid ncADCs were sutided using a luciferase reporter cell based assay using either the 293/PRLR/GRE-luc cell line described in Example 62 as well as an antigen negative 293 cell line that contains a chimeric receptor consisting of a GR ligand binding domain fused to the yeast CAL4 DNA binding domain (pBind-GR, Promega catalog no. E1581), and a Gal4 upstram activator sequence (9XGal4 UAS-Luc) that drives luciferase expression.

Article Title: Phosphodiesterase 5 inhibitor mirodenafil ameliorates Alzheimer-like pathology and symptoms by multimodal actions
Article Snippet: For the GR reporter gene assay, HT-22 or HEK293 cells were seeded on a 96-well plate and co-transfected with pGL4.35[luc2P/9XGAL4UAS/Hygro] vector (Promega) and pBIND-GR (Promega) vector using Fugene HD reagent for 24 h at 37°C in a CO 2 incubator.

Article Title: Steroids and protein-conjugates thereof
Article Snippet: Then such stable cell line was further transfected with a chimeric receptor consisting of a GR ligand binding domain fused to the yeast Gal4 DNA binding domain (pBind-GR, Promega Cat #E1581), and a Gal4 upstream activator sequence (9×Gal4UAS-Luc2P) that drives luciferase gene expression.

Article Title: Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport
Article Snippet: Analyses of GR translocation to the nucleus Approximately 1.25x10 5 PC3 cells and 5.0x10 5 293T cells on 24-well plates were transfected with 100 ng pMACS Kk.HA-C-SGTA, pMACS Kk.HA-C-REIC/Dkk-3, pGFP-V-RS containing SGTA-specific shRNA, pBIND-GR containing the GR ligand-biding domain and renilla luciferase reporter gene (cat. no. E158A; Promega), or pGL4.35(luc2P/9XGAL4 UAS/Hygro) containing the firefly luciferase reporter gene (cat. no. E137A; Promega).




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