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pb1189  (ATCC)


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  • 90

    Structured Review

    ATCC pb1189
    Pb1189, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pb1189/pLDR8/us10487334-428-34-41
    Average 90 stars, based on 6 article reviews
    pb1189 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Antibiotic-free plasmid
    Article Snippet: The DNA fragment corresponding to the P1 weak promoter was obtained by PCR using primers PB1186 (SEQ ID NO:35) (TCATACCAGGCCTAGGTGATACGCCTATTTTTATAGGTTAATG) and PB1187 (SEQ ID NO:36) (AACACCCCTTGTATTACTGTTTATG), using pLL14 (see Merial patent application US2005/0164946) as a template and Phusion DNA polymerase. .. The DNA fragment corresponding to the cI gene (SEQ ID NO:1) was obtained by PCR using primers PB1188 (SEQ ID NO:37) (AATACAAGGGGTGTTATGAGCACAAAAAAGAAACCATTAACAC) [containing a complementary region of P1 sequence at 5′ end (underlined)] and PB1189 (SEQ ID NO:38) (CCGGAATTCGGCGCGTCAGCCAAACGTCTCTTCAGGCCACTG) using pLDR8 (ATCC #77357) as a template and Phusion DNA polymerase. .. The two PCR products (respectively 151 and 729 bp) were purified and used as template in a second PCR step with the PB1186 and PB1189 primers and the Phusion DNA polymerase (Finnzymes, Finland) (see FIG. 24).

    Article Title: Antibiotic-free plasmids
    Article Snippet: The DNA fragment corresponding to the P1 weak promoter was obtained by PCR using primers PB1186 (SEQ ID NO:35) (TCATACCAGGCCTAGGTGATACGCCTATTTTTATAGGTTAATG) and PB1187 (SEQ ID NO:36) (AACACCCCTTGTATTACTGTTTATG), using pLL14 (see Merial patent application US2005/0164946) as a template and Phusion DNA polymerase. .. The DNA fragment corresponding to the cI gene (SEQ ID NO:1) was obtained by PCR using primers PB1188 (SEQ ID NO:37) (AATACAAGGGGTGTTATGAGCACAAAAAAGAAACCATTAACAC) [containing a complementary region of Plsequence at 5′ end (underlined)] and PB1189 (SEQ ID NO:38) (CCGGAATTCGGCGCGTCAGCCAAACGTCTCTTCAGGCCACTG) using pLDR8 (ATCC # 77357) as a template and Phusion DNA polymerase. .. The two PCR products (respectively 151 and 729 bp) were purified and used as template in a second PCR step with the PB1186 and PB1189 primers and the Phusion DNA polymerase (Finnzymes, Finland) (see FIG. 24).

    Sequencing:

    Article Title: Antibiotic-free plasmid
    Article Snippet: The DNA fragment corresponding to the P1 weak promoter was obtained by PCR using primers PB1186 (SEQ ID NO:35) (TCATACCAGGCCTAGGTGATACGCCTATTTTTATAGGTTAATG) and PB1187 (SEQ ID NO:36) (AACACCCCTTGTATTACTGTTTATG), using pLL14 (see Merial patent application US2005/0164946) as a template and Phusion DNA polymerase. .. The DNA fragment corresponding to the cI gene (SEQ ID NO:1) was obtained by PCR using primers PB1188 (SEQ ID NO:37) (AATACAAGGGGTGTTATGAGCACAAAAAAGAAACCATTAACAC) [containing a complementary region of P1 sequence at 5′ end (underlined)] and PB1189 (SEQ ID NO:38) (CCGGAATTCGGCGCGTCAGCCAAACGTCTCTTCAGGCCACTG) using pLDR8 (ATCC #77357) as a template and Phusion DNA polymerase. .. The two PCR products (respectively 151 and 729 bp) were purified and used as template in a second PCR step with the PB1186 and PB1189 primers and the Phusion DNA polymerase (Finnzymes, Finland) (see FIG. 24).



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    pb1189  (ATCC)
    90
    ATCC pb1189
    Pb1189, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pb1189/pLDR8/us10487334-428-34-41
    Average 90 stars, based on 1 article reviews
    pb1189 - by Bioz Stars, 2026-10
    90/100 stars
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