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1x passive lysis buffer  (Biotium)


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    Structured Review

    Biotium 1x passive lysis buffer
    1x Passive Lysis Buffer, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passive/Passive+Lysis+Buffer/bio_rxiv__64898__2026__10__05__756672-135-3-7
    Average 94 stars, based on 46 article reviews
    1x passive lysis buffer - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Lysis:

    Article Title: Structural basis of eIF4E recognition of synthetic mRNA 5′ cap analogues links cap architecture to affinity and biological activity
    Article Snippet: .. 100 μL of 1x passive lysis buffer (Biotium) was added to each well. .. Cells were shaken for 15 min at room temperature and then stored at -80°C for at least 1 h. After thawing, 20 μL of lysates were used to detect and measure luciferase activity via chemiluminescence using adenosine triphosphate (ATP) and D-luciferin in a Beetlejuice buffer system (p.j.k.).

    Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein
    Article Snippet: HEK293T cells were transfected with M50 Super 8x TOPFlash (Addgene #12456) or the FOP control plasmid (M51 Super 8x FOPFlash Addgene #12457) in combination with TK Renilla (Promega #E2241) using DreamFect Gold (OZ Biosciences #DG80500) according to the manufacturer's instructions. .. After 24 h, cells were incubated with starvation media (Opti‐MEM reduced serum medium supplemented with 0.5% FBS, 1% penicillin/streptomycin, 1% sodium pyruvate, and 1% nonessential amino acids) for 8 h. After starvation, Wnt/β‐catenin signaling was activated by treating cells with 50 mM LiCl in starvation media for 24 h. The following day, cells were treated with 15 or vehicle (DMSO) for 24 h. At the end of the experiment, cells were lysed using Passive Lysis Buffer (Biotium #99912). .. HEK293T cells were transfected with M50 Super 8x TOPFlash (Addgene #12456) or the FOP control plasmid (M51 Super 8x FOPFlash Addgene #12457) in combination with TK Renilla (Promega #E2241) using DreamFect Gold (OZ Biosciences #DG80500) according to the manufacturer's instructions.

    Article Title: The differential effect of SARS-CoV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage, and virus evolution.
    Article Snippet: After 1 h adsortion, the inoculum was removed nd cells were transfected with a combination of pEGFP, pRL- and pFFL plasmids (0.25 μg each) using Lipofectamine TM 000 (Invitrogen). .. Cell extracts were prepared at 16- or 24- post-infection (hpi) for luciferase activity measurement and estern blot. easurement of reporter gene expression nzymatic measurements were carried out in cell extracts preared in passive lysis buffer (Biotium). .. Firefly, renilla, and anoLuc luciferases were measured in a Berthold luminome- er using D-luciferin (Promega), collantrazine (Biotium), and ano-Glo (Promega), respectively.

    Article Title: The differential effect of SARS-CoV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage, and virus evolution
    Article Snippet: .. Enzymatic measurements were carried out in cell extracts prepared in passive lysis buffer (Biotium). .. Firefly, renilla, and NanoLuc luciferases were measured in a Berthold luminometer using D-luciferin (Promega), collantrazine (Biotium), and Nano-Glo (Promega), respectively.

    Incubation:

    Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein
    Article Snippet: HEK293T cells were transfected with M50 Super 8x TOPFlash (Addgene #12456) or the FOP control plasmid (M51 Super 8x FOPFlash Addgene #12457) in combination with TK Renilla (Promega #E2241) using DreamFect Gold (OZ Biosciences #DG80500) according to the manufacturer's instructions. .. After 24 h, cells were incubated with starvation media (Opti‐MEM reduced serum medium supplemented with 0.5% FBS, 1% penicillin/streptomycin, 1% sodium pyruvate, and 1% nonessential amino acids) for 8 h. After starvation, Wnt/β‐catenin signaling was activated by treating cells with 50 mM LiCl in starvation media for 24 h. The following day, cells were treated with 15 or vehicle (DMSO) for 24 h. At the end of the experiment, cells were lysed using Passive Lysis Buffer (Biotium #99912). .. HEK293T cells were transfected with M50 Super 8x TOPFlash (Addgene #12456) or the FOP control plasmid (M51 Super 8x FOPFlash Addgene #12457) in combination with TK Renilla (Promega #E2241) using DreamFect Gold (OZ Biosciences #DG80500) according to the manufacturer's instructions.

    Luciferase:

    Article Title: The differential effect of SARS-CoV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage, and virus evolution.
    Article Snippet: After 1 h adsortion, the inoculum was removed nd cells were transfected with a combination of pEGFP, pRL- and pFFL plasmids (0.25 μg each) using Lipofectamine TM 000 (Invitrogen). .. Cell extracts were prepared at 16- or 24- post-infection (hpi) for luciferase activity measurement and estern blot. easurement of reporter gene expression nzymatic measurements were carried out in cell extracts preared in passive lysis buffer (Biotium). .. Firefly, renilla, and anoLuc luciferases were measured in a Berthold luminome- er using D-luciferin (Promega), collantrazine (Biotium), and ano-Glo (Promega), respectively.

    Activity Assay:

    Article Title: The differential effect of SARS-CoV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage, and virus evolution.
    Article Snippet: After 1 h adsortion, the inoculum was removed nd cells were transfected with a combination of pEGFP, pRL- and pFFL plasmids (0.25 μg each) using Lipofectamine TM 000 (Invitrogen). .. Cell extracts were prepared at 16- or 24- post-infection (hpi) for luciferase activity measurement and estern blot. easurement of reporter gene expression nzymatic measurements were carried out in cell extracts preared in passive lysis buffer (Biotium). .. Firefly, renilla, and anoLuc luciferases were measured in a Berthold luminome- er using D-luciferin (Promega), collantrazine (Biotium), and ano-Glo (Promega), respectively.

    Gene Expression:

    Article Title: The differential effect of SARS-CoV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage, and virus evolution.
    Article Snippet: After 1 h adsortion, the inoculum was removed nd cells were transfected with a combination of pEGFP, pRL- and pFFL plasmids (0.25 μg each) using Lipofectamine TM 000 (Invitrogen). .. Cell extracts were prepared at 16- or 24- post-infection (hpi) for luciferase activity measurement and estern blot. easurement of reporter gene expression nzymatic measurements were carried out in cell extracts preared in passive lysis buffer (Biotium). .. Firefly, renilla, and anoLuc luciferases were measured in a Berthold luminome- er using D-luciferin (Promega), collantrazine (Biotium), and ano-Glo (Promega), respectively.



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