Review



p414gall  (ATCC)


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  • 90

    Structured Review

    ATCC p414gall
    P414gall, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p414gall/p414+GALL/10__1128_slash_mcb__01226___07-51-24-25
    Average 90 stars, based on 2 article reviews
    p414gall - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Proline-Rich Transcript in Brain Protein Induces Stress Granule Formation
    Article Snippet: To construct the pBCT-eIF4GII (amino acids [aa] 1 to 145) plasmid used in the Y2H screening, pSK(−)-eIF4GII ( 18 ) was digested with restriction endonucleases SmaI and BamHI, followed by being filled in with a Klenow fragment (New England Biolabs). .. The expression level of bait protein was controlled using pBCT plasmid, in which the 2μ region of pGBT9 (Clontech) is exchanged for CEN6/ARSH4 of p414GALL (ATCC) through AatII and NsiI sites. ..

    Article Title: Proline-Rich Transcript in Brain Protein Induces Stress Granule Formation
    Article Snippet: To construct the pBCT-eIF4GII (amino acids [aa] 1 to 145) plasmid used in the Y2H screening, pSK( )-eIF4GII (18) was digested with restriction endonucleases SmaI and BamHI, followed by being filled in with a Klenow fragment (New England Biolabs). .. The expression level of bait protein was controlled using pBCT plasmid, in which the 2 region of pGBT9 (Clontech) is exchanged for CEN6/ARSH4 of p414GALL (ATCC) through AatII and NsiI sites. ..

    Plasmid Preparation:

    Article Title: Proline-Rich Transcript in Brain Protein Induces Stress Granule Formation
    Article Snippet: To construct the pBCT-eIF4GII (amino acids [aa] 1 to 145) plasmid used in the Y2H screening, pSK(−)-eIF4GII ( 18 ) was digested with restriction endonucleases SmaI and BamHI, followed by being filled in with a Klenow fragment (New England Biolabs). .. The expression level of bait protein was controlled using pBCT plasmid, in which the 2μ region of pGBT9 (Clontech) is exchanged for CEN6/ARSH4 of p414GALL (ATCC) through AatII and NsiI sites. ..

    Article Title: Proline-Rich Transcript in Brain Protein Induces Stress Granule Formation
    Article Snippet: To construct the pBCT-eIF4GII (amino acids [aa] 1 to 145) plasmid used in the Y2H screening, pSK( )-eIF4GII (18) was digested with restriction endonucleases SmaI and BamHI, followed by being filled in with a Klenow fragment (New England Biolabs). .. The expression level of bait protein was controlled using pBCT plasmid, in which the 2 region of pGBT9 (Clontech) is exchanged for CEN6/ARSH4 of p414GALL (ATCC) through AatII and NsiI sites. ..

    Article Title: Mdv1 Interacts with Assembled Dnm1 to Promote Mitochondrial Division
    Article Snippet: .. Plasmids—A plasmid containing GAL-regulated MDV1 was constructed by inserting theKpnI-SacI piece of p416GALL (AmericanType Culture Collection) containing the GALL promoter and MCS into pRS314 to create p414GALL. .. GFP-MDV1 was removed from p416GALL-MDV1 (17) by SpeI and XhoI digest and inserted in p414GALL resulting in p414GALL-GFP-MDV1. p416GALL-dsRed/dimer2-MDV1 was created by ligating MDV1 into p416GALL (American Type Culture Collection) using BamHI and EcoRI sites.

    Construct:

    Article Title: Mdv1 Interacts with Assembled Dnm1 to Promote Mitochondrial Division
    Article Snippet: .. Plasmids—A plasmid containing GAL-regulated MDV1 was constructed by inserting theKpnI-SacI piece of p416GALL (AmericanType Culture Collection) containing the GALL promoter and MCS into pRS314 to create p414GALL. .. GFP-MDV1 was removed from p416GALL-MDV1 (17) by SpeI and XhoI digest and inserted in p414GALL resulting in p414GALL-GFP-MDV1. p416GALL-dsRed/dimer2-MDV1 was created by ligating MDV1 into p416GALL (American Type Culture Collection) using BamHI and EcoRI sites.



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