p4146 (Teknova)
Structured Review
P4146, supplied by Teknova, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p4146/20%25+PEG+8000+with+2%2E5M+Sodium+Chloride%2E+DNase+and+RNase+Tested/pm36206356-281-40-38
Average 92 stars, based on 3 article reviews
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Binding Assay:Article Title: Evolution of antigen-specific follicular helper T cell transcription from effector function to memory. Article Snippet: After pooling, 0.3 μl of Exo-SAP-IT express was added per 1 μl of pooled library; thermocycling conditions were 20 min at 37°C followed by heat inactivation at 80°C for 2 min. Volume reduction and additional oligoDT removal was performed using AMpureXP SPRIselect paramagnetic beads (BeckmanCoulter) at a 0.8:1 bead-tolibrary ratio with our pooled library. .. To reduce the quantity of paramagnetic beads used per plate, we used 100 μl of beads per library with pure bead binding buffer for the remaining volume [Bead Binding Buffer, 2.5 M NaCl, and 20% (vol/vol) polyethylene glycol; |
