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p16 10883 1 ap rabbit  (Proteintech)


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    Structured Review

    Proteintech p16 10883 1 ap rabbit
    P16 10883 1 Ap Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p16/P16-INK4A+Antibody/pm42829902-140-46-51
    Average 96 stars, based on 750 article reviews
    p16 10883 1 ap rabbit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Novel PMVs / ZIP4 /Zinc/Prelamin A Axis Promotes Nuclear Dysmorphism and Vascular Aging in Humans and Rodents Post‐Injury: Effective Treatment With Platelet Membrane‐Coated ZIF ‐8 Nanoparticles
    Article Snippet: .. Subsequently, the sections were incubated with the primary antibodies against CD41 (1:100, Abcam, Cambridge, UK), Lamin A (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), prelamin A (1:200, Sigma‐Aldrich, USA), Tri‐Methyl Histone 3 lysine 9 (H3K9me3) (1:200, Abcam, USA), Acetyl‐Histone H3 lysine 9 (H3K9ac) (1:200, Cell Signaling Technology, USA), p16 (1:500, Proteintech, China), p21 (1:500, Proteintech, China), and phosphorylated p53 (P‐p53; 1:1000, Cell Signaling Technology, USA) at 4°C overnight. ..

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration
    Article Snippet: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 system. .. After blocked with 5% non-fat milk for 2 h at room temperature, the membranes were incubated with primary antibodies against GAPDH (1:5000, 104941-AP, Proteintech), TSG101 (1:1000, DF8427, Affinity), CD9 (1:1000, AF5139, Affinity), CD63 (1:2000, 25682-1-AP, Proteintech), Calnexin (1:5000, 10427-2-AP, Proteintech), GM130 (1:20000, 11308-1-AP, Proteintech), CXCR3 (1:5000, 26756-1-AP, Proteintech), CXCL10 (1:2000, 10937-1-AP, Proteintech), MMP3 (1:2000, 17873-1-AP, Proteintech), ADAMTS5 (DF13268, Affinity), P16 (AF5484, Affinity), P21 (10355-1-AP, Proteintech), GPX4 (1:1000, 381958, Zen-bio), SLC7A11 (1:1000, 26864-1-AP, Proteintech), ACSL4 (1:5000, 22401-1-AP, Proteintech) and Tubulin (1:10000, T40103 , Abmart) overnight at 4 °C. .. After washing three times with TBST (Tris-buffered saline and 0.1% Tween 20), the membranes were incubated with secondary antibodies (1:5000, SA00001-2, SA00001-1, Proteintech) for 2 h at room temperature.

    Western Blot:

    Article Title: AP39 alleviates HHCY-induced myocardial remodeling by regulating FUNDC1-mediated mitochondrial dynamics via S-sulfhydration of NEDD8/CUL4B
    Article Snippet: .. All primary antibodies used for Western blotting (WB) and immunofluorescence (IF) were purchased from the corresponding commercial suppliers, and their detailed information is listed as follows: GAPDH (Proteintech, USA; 10494-1-AP; WB 1:8000), α-smooth muscle actin (α-SMA) (Proteintech, USA; 14395-1-AP; WB 1:4000), Collagen type III (Proteintech, USA; 22734-1-AP; WB 1:800), FUNDC1 (Proteintech, China; AWA51295 ; IF 1:100, WB 1:1000), LC3B (Proteintech, USA; 14600-1-AP; IF 1:500, WB 1:4000), MFN1 (Proteintech, USA; 13798-1-AP; WB 1:5000), MFN2 (Proteintech, USA; 12186-1-AP; WB 1:20000), DRP1 (Proteintech, USA; 12957-1-AP; WB 1:5000), P53 (Proteintech, USA; 10442-1-AP; WB 1:20000), P16 (Proteintech, USA; 10883-1-AP; WB 1:3000), NEDD8 (Abways, China; AWA11003 ; WB 1:1000), and CUL4B (Abways, China; AWA48229 ; WB 1:1000). .. For secondary antibodies, HRP-conjugated goat anti-mouse/goat anti-rabbit IgG (Proteintech, USA; SA00001-2; 1:5000) was used for Western blot detection.

    Article Title: The multilayer coatings on polylactic acid implants spatiotemporally regulates the microenvironment to enhance antibacterial and osseointegration capacity
    Article Snippet: Antibodies against CD86 and CD206 for immunofluorescence were obtained from Novus Biologicals (Littleton, USA) and Proteintech (Chicago, USA). .. Antibodies of CD206, RUNX2, OPN, OCN, P16, Cyclin D1, TNF-α, CCL2 for immunofluorescence and Western Blot were bought from Proteintech (Chicago, USA). .. Secondary antibodies against anti-rabbit IgG (H + L), F(ab')2 Fragment (Alexa Fluor 488 Conjugate), anti-rabbit IgG (H + L), F(ab')2 Fragment (Alexa Fluor 594 Conjugate), Anti-rabbit IgG, horseradish peroxidase (HRP)-conjugated antibody were obtained from Proteintech (Chicago, USA).

    Immunofluorescence:

    Article Title: AP39 alleviates HHCY-induced myocardial remodeling by regulating FUNDC1-mediated mitochondrial dynamics via S-sulfhydration of NEDD8/CUL4B
    Article Snippet: .. All primary antibodies used for Western blotting (WB) and immunofluorescence (IF) were purchased from the corresponding commercial suppliers, and their detailed information is listed as follows: GAPDH (Proteintech, USA; 10494-1-AP; WB 1:8000), α-smooth muscle actin (α-SMA) (Proteintech, USA; 14395-1-AP; WB 1:4000), Collagen type III (Proteintech, USA; 22734-1-AP; WB 1:800), FUNDC1 (Proteintech, China; AWA51295 ; IF 1:100, WB 1:1000), LC3B (Proteintech, USA; 14600-1-AP; IF 1:500, WB 1:4000), MFN1 (Proteintech, USA; 13798-1-AP; WB 1:5000), MFN2 (Proteintech, USA; 12186-1-AP; WB 1:20000), DRP1 (Proteintech, USA; 12957-1-AP; WB 1:5000), P53 (Proteintech, USA; 10442-1-AP; WB 1:20000), P16 (Proteintech, USA; 10883-1-AP; WB 1:3000), NEDD8 (Abways, China; AWA11003 ; WB 1:1000), and CUL4B (Abways, China; AWA48229 ; WB 1:1000). .. For secondary antibodies, HRP-conjugated goat anti-mouse/goat anti-rabbit IgG (Proteintech, USA; SA00001-2; 1:5000) was used for Western blot detection.

    Article Title: The multilayer coatings on polylactic acid implants spatiotemporally regulates the microenvironment to enhance antibacterial and osseointegration capacity
    Article Snippet: Antibodies against CD86 and CD206 for immunofluorescence were obtained from Novus Biologicals (Littleton, USA) and Proteintech (Chicago, USA). .. Antibodies of CD206, RUNX2, OPN, OCN, P16, Cyclin D1, TNF-α, CCL2 for immunofluorescence and Western Blot were bought from Proteintech (Chicago, USA). .. Secondary antibodies against anti-rabbit IgG (H + L), F(ab')2 Fragment (Alexa Fluor 488 Conjugate), anti-rabbit IgG (H + L), F(ab')2 Fragment (Alexa Fluor 594 Conjugate), Anti-rabbit IgG, horseradish peroxidase (HRP)-conjugated antibody were obtained from Proteintech (Chicago, USA).



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    CI in older and D‐gal‐induced mice is accompanied by microglial senescence and elevated ghrelin levels. (A) Experimental design of the D‐gal‐induced aging mouse model. (B) Percentage change in body weight of mice in the Young, Old, Control, and D‐gal groups, normalized to baseline body weight at the start of the experiment. (C) The NOL test comparing the mice from the Young and Old groups and from the Control and D‐gal groups. The recognition index was calculated as the ratio of time spent exploring in the novel location to the total time spent exploring both locations. (D) NOR test comparing the mice from the Young and Old groups and from the Control and D‐gal groups. The recognition index was calculated as the ratio of time spent exploring the novel object relative to the total exploration time for both objects. (E) MWM test comparing the mice from the Young and Old groups and from the Control and D‐gal groups, including swimming trajectories and escape latency. (F) Western blot analysis of senescence markers p16 Ink4a , p21 Cip1 , LaminB1, γH2AX, and HMGB1 in the mice brain tissue from the Young, Old, Control, and D‐gal groups. (G) Immunofluorescence co‐localization of Iba‐1 + microglia with LaminB1, γH2AX, and HMGB1 in the hippocampal CA1 and CA3 regions of the mice from Young, Old, Control, and D‐gal groups (magnification: ×400, scale bar = 25 μm). (H) ELISA‐based quantification of hippocampal cytokines (IL‐1α, IL‐1β, G‐CSF, IL‐4, IL‐6, IL‐8, CXCL10, LIF, MCP1, and M‐CSF) in the Young, Old, Control, and D‐gal groups. (I) ELISA measurement of plasma total ghrelin and acylated ghrelin in the Young, Old, Control, and D‐gal groups. ** p < 0.01, *** p < 0.001.

    Journal: Aging Cell

    Article Title: Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia

    doi: 10.1111/acel.70732

    Figure Lengend Snippet: CI in older and D‐gal‐induced mice is accompanied by microglial senescence and elevated ghrelin levels. (A) Experimental design of the D‐gal‐induced aging mouse model. (B) Percentage change in body weight of mice in the Young, Old, Control, and D‐gal groups, normalized to baseline body weight at the start of the experiment. (C) The NOL test comparing the mice from the Young and Old groups and from the Control and D‐gal groups. The recognition index was calculated as the ratio of time spent exploring in the novel location to the total time spent exploring both locations. (D) NOR test comparing the mice from the Young and Old groups and from the Control and D‐gal groups. The recognition index was calculated as the ratio of time spent exploring the novel object relative to the total exploration time for both objects. (E) MWM test comparing the mice from the Young and Old groups and from the Control and D‐gal groups, including swimming trajectories and escape latency. (F) Western blot analysis of senescence markers p16 Ink4a , p21 Cip1 , LaminB1, γH2AX, and HMGB1 in the mice brain tissue from the Young, Old, Control, and D‐gal groups. (G) Immunofluorescence co‐localization of Iba‐1 + microglia with LaminB1, γH2AX, and HMGB1 in the hippocampal CA1 and CA3 regions of the mice from Young, Old, Control, and D‐gal groups (magnification: ×400, scale bar = 25 μm). (H) ELISA‐based quantification of hippocampal cytokines (IL‐1α, IL‐1β, G‐CSF, IL‐4, IL‐6, IL‐8, CXCL10, LIF, MCP1, and M‐CSF) in the Young, Old, Control, and D‐gal groups. (I) ELISA measurement of plasma total ghrelin and acylated ghrelin in the Young, Old, Control, and D‐gal groups. ** p < 0.01, *** p < 0.001.

    Article Snippet: Following antibody stripping and re‐blocking, sections were incubated overnight at 4°C with a second primary antibody: γH2AX (1:400; ab81299, Abcam), high‐mobility group box 1 (HMGB1) (1:400; AWA10032 , Abiowell), p21 Cip1 (1:200; ab188224, Abcam), p16 Ink4a (1:200; bs‐23797R, Bioss, China), or Lamin B1 (1:400; ab8982, Abcam).

    Techniques: Control, Western Blot, Analysis, Tissue, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

    Ghrelin administration alleviates D‐gal‐induced microglial senescence and CI. (A) Experimental design of ghrelin treatment in the D‐gal‐induced aging mouse model. (B) Percentage change in body weight of mice in the Control, Control + Ghrelin, D‐gal, and D‐gal + Ghrelin groups, normalized to baseline body weight at the start of the experiment. (C) The NOL test comparing the mice from the Control, Control + Ghrelin, D‐gal, and D‐gal + Ghrelin groups. The recognition index was calculated as the ratio of time spent exploring in the novel location to the total time spent exploring both locations. (D) NOR test comparing the mice from the Control, Control + Ghrelin, D‐gal, and D‐gal+Ghrelin groups. The recognition index was calculated as the ratio of time spent exploring the novel object relative to the total exploration time for both objects. (E) MWM test comparing the mice from the Control, Control + Ghrelin, D‐gal, and D‐gal + Ghrelin groups, including swimming trajectories and escape latency. (F) Western blot analysis of senescence markers p16 Ink4a , p21 Cip1 , LaminB1, γH2AX, and HMGB1 in the mice brain tissue from the Control, Control+Ghrelin, D‐gal, and D‐gal+Ghrelin groups. (G) Immunofluorescence co‐localization of Iba‐1 + microglia with LaminB1, γH2AX, and HMGB1 in the hippocampal CA1 region of the mice from Control, Control+Ghrelin, D‐gal, and D‐gal + Ghrelin groups (magnification: ×400, scale bar = 25 μm). (H) ELISA‐based quantification of hippocampal cytokines (IL‐1α, IL‐1β, G‐CSF, IL‐4, IL‐6, IL‐8, IP10/CXCL10, LIF, MCP1, and M‐CSF) in the Control, Control + Ghrelin, D‐gal, and D‐gal + Ghrelin groups. *** p < 0.001.

    Journal: Aging Cell

    Article Title: Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia

    doi: 10.1111/acel.70732

    Figure Lengend Snippet: Ghrelin administration alleviates D‐gal‐induced microglial senescence and CI. (A) Experimental design of ghrelin treatment in the D‐gal‐induced aging mouse model. (B) Percentage change in body weight of mice in the Control, Control + Ghrelin, D‐gal, and D‐gal + Ghrelin groups, normalized to baseline body weight at the start of the experiment. (C) The NOL test comparing the mice from the Control, Control + Ghrelin, D‐gal, and D‐gal + Ghrelin groups. The recognition index was calculated as the ratio of time spent exploring in the novel location to the total time spent exploring both locations. (D) NOR test comparing the mice from the Control, Control + Ghrelin, D‐gal, and D‐gal+Ghrelin groups. The recognition index was calculated as the ratio of time spent exploring the novel object relative to the total exploration time for both objects. (E) MWM test comparing the mice from the Control, Control + Ghrelin, D‐gal, and D‐gal + Ghrelin groups, including swimming trajectories and escape latency. (F) Western blot analysis of senescence markers p16 Ink4a , p21 Cip1 , LaminB1, γH2AX, and HMGB1 in the mice brain tissue from the Control, Control+Ghrelin, D‐gal, and D‐gal+Ghrelin groups. (G) Immunofluorescence co‐localization of Iba‐1 + microglia with LaminB1, γH2AX, and HMGB1 in the hippocampal CA1 region of the mice from Control, Control+Ghrelin, D‐gal, and D‐gal + Ghrelin groups (magnification: ×400, scale bar = 25 μm). (H) ELISA‐based quantification of hippocampal cytokines (IL‐1α, IL‐1β, G‐CSF, IL‐4, IL‐6, IL‐8, IP10/CXCL10, LIF, MCP1, and M‐CSF) in the Control, Control + Ghrelin, D‐gal, and D‐gal + Ghrelin groups. *** p < 0.001.

    Article Snippet: Following antibody stripping and re‐blocking, sections were incubated overnight at 4°C with a second primary antibody: γH2AX (1:400; ab81299, Abcam), high‐mobility group box 1 (HMGB1) (1:400; AWA10032 , Abiowell), p21 Cip1 (1:200; ab188224, Abcam), p16 Ink4a (1:200; bs‐23797R, Bioss, China), or Lamin B1 (1:400; ab8982, Abcam).

    Techniques: Control, Western Blot, Analysis, Tissue, Immunofluorescence, Enzyme-linked Immunosorbent Assay