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anti ocn osteocalcin  (Proteintech)


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    Structured Review

    Proteintech anti ocn osteocalcin
    Anti Ocn Osteocalcin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 440 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ocn/Osteocalcin+Antibody/pm42869321-157-22-25
    Average 96 stars, based on 440 article reviews
    anti ocn osteocalcin - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: An aligned-to-random PLA/PCL/COL1 bilayer electrospun scaffold loaded with exosomes of bone marrow-derived mesenchymal stem cells promotes tendon-to-bone healing in rats.
    Article Snippet: The regeneration of tendon-to-bone interface remains a significant clinical challenge due to the highly complex structure, composition, cell population, and mechanical properties of the interface.. In this study, polylactic acid (PLA), polycaprolactone (PCL) and type I collagen (Col1) were used as raw materials to prepare a tendon-to-bone interface biomimetic scaffold through electrospinning technology.. The nanofiber scaffold features an aligned-torandom bilayer structure mimicking the native ten-don-to-bone interface.

    Article Title: A dual-functional Janus nanofibrous membrane as an immunomodulatory barrier for periodontitis regeneration under diabetic conditions
    Article Snippet: .. For immunohistochemical (IHC) analysis, sections were incubated with the following primary antibodies: OCN (Proteintech, 16157-1-AP), RUNX2 (Proteintech, 207001-1-AP), IL-6 (Proteintech,26404-1-AP), anti-TNF-α (Proteintech, 17590-1-AP), and anti-IL-1β (Proteintech,66737-1-lg). ..

    Article Title: TRPV4-mediated mechanotransduction governs surface topography-induced bone formation via macrophage-osteoclast axis
    Article Snippet: An inorganic tri‐calcium phosphate ceramic (TCP) with a submicron surface topography (TCP–S) gives rise to bone formation in ectopic sites, while its counterpart with micron surface topography (TCP–B) does not.. We hypothesized that mechanosensitive ion channels act as converters from physical cues (generated by surface topography) to biological responses (bone formation).. Compared to TCP–B, TCP-S activated integrin-mediated focal adhesion complexes, enhanced cytoskeletal tension, and subsequently triggered the Transient Receptor Potential Vanilloid 4 (TRPV4) channel, leading to Ca2+ influx of macrophages in vitro.

    Article Title: Moringa oleifera Leaf-Derived Exosome-Like Nanoparticles Target Macrophage Senescence to Drive M2 Polarisation and Attenuate Alveolar Bone Loss
    Article Snippet: Histological analyses included H&E staining for general morphology, Masson’s trichrome staining to evaluate collagen fibre organisation in periodontal tissues and TRAP staining (Servicebio GB1050 kit) to identify osteoclasts. .. For immunohistochemistry, sections underwent antigen retrieval, followed by overnight incubation at 4 °C with primary antibodies against IL-1b, TNF-a, IL-10, OCN and COL-1 (Proteintech, 1:100). .. After incubation with an HRP-conjugated secondary antibody (Servicebio, GB23303) at room temperature, DAB chromogen and haematoxylin counterstaining were applied to visualise inflammation- and osteogenesis-related factors.

    Immunohistochemical staining:

    Article Title: A dual-functional Janus nanofibrous membrane as an immunomodulatory barrier for periodontitis regeneration under diabetic conditions
    Article Snippet: .. For immunohistochemical (IHC) analysis, sections were incubated with the following primary antibodies: OCN (Proteintech, 16157-1-AP), RUNX2 (Proteintech, 207001-1-AP), IL-6 (Proteintech,26404-1-AP), anti-TNF-α (Proteintech, 17590-1-AP), and anti-IL-1β (Proteintech,66737-1-lg). ..

    Immunohistochemistry:

    Article Title: A dual-functional Janus nanofibrous membrane as an immunomodulatory barrier for periodontitis regeneration under diabetic conditions
    Article Snippet: .. For immunohistochemical (IHC) analysis, sections were incubated with the following primary antibodies: OCN (Proteintech, 16157-1-AP), RUNX2 (Proteintech, 207001-1-AP), IL-6 (Proteintech,26404-1-AP), anti-TNF-α (Proteintech, 17590-1-AP), and anti-IL-1β (Proteintech,66737-1-lg). ..

    Article Title: Moringa oleifera Leaf-Derived Exosome-Like Nanoparticles Target Macrophage Senescence to Drive M2 Polarisation and Attenuate Alveolar Bone Loss
    Article Snippet: Histological analyses included H&E staining for general morphology, Masson’s trichrome staining to evaluate collagen fibre organisation in periodontal tissues and TRAP staining (Servicebio GB1050 kit) to identify osteoclasts. .. For immunohistochemistry, sections underwent antigen retrieval, followed by overnight incubation at 4 °C with primary antibodies against IL-1b, TNF-a, IL-10, OCN and COL-1 (Proteintech, 1:100). .. After incubation with an HRP-conjugated secondary antibody (Servicebio, GB23303) at room temperature, DAB chromogen and haematoxylin counterstaining were applied to visualise inflammation- and osteogenesis-related factors.

    Analysis:

    Article Title: A dual-functional Janus nanofibrous membrane as an immunomodulatory barrier for periodontitis regeneration under diabetic conditions
    Article Snippet: .. For immunohistochemical (IHC) analysis, sections were incubated with the following primary antibodies: OCN (Proteintech, 16157-1-AP), RUNX2 (Proteintech, 207001-1-AP), IL-6 (Proteintech,26404-1-AP), anti-TNF-α (Proteintech, 17590-1-AP), and anti-IL-1β (Proteintech,66737-1-lg). ..

    Concentration Assay:

    Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs
    Article Snippet: .. The information and the concentration of antibodies used in this study were listed as follows: Phospho-(Ser/Thr) PKA substrate antibody (1: 500, cat. no. 9621, CST, USA), GLP1R (1: 2,000, cat. no. 26196-1-AP, Proteintech, China), GIPR (1: 2,000, cat. no. 28322-1-AP, Proteintech), STRO-1 (1: 250, cat. no. 39-8401, Thermo Fisher Scientific), CD146 (1: 2,000, cat. no. 65181-1-Ig, Proteintech), Vimentin (1: 2,000, cat. no. 10366-1-AP, Proteintech), CREB1 (1: 2,000, cat. no. 67927-1-Ig, Proteintech), p-CREB1 (Ser133) (1: 2,000, cat. no. 28792-1-AP, Proteintech), ERK1/2 (1: 2,000, cat. no. 66192-1-Ig, Proteintech), p-ERK1/2 (Thr202/Tyr204) (1: 2,000, cat. no. 28733-1-AP, Proteintech), β-catenin (1: 2,000, cat. no. 51067-2-AP, Proteintech), p-β-catenin (Ser33) (1: 2,000, cat. no. 80067-1-RR, Proteintech), STAT3 (1: 2,000, cat. no. 10253-2-AP, Proteintech), p-STAT3 (Ser727) (1: 2,000, cat. no. 80199-2-RR, Proteintech), Ki-67 (1: 2,000, cat. no. 84192-4-RR, Proteintech), PCNA (1: 2,000, cat. no. 10205-2-AP, Proteintech), CCND3 (1: 2,000, cat. no. 26755-1-AP, Proteintech), β-actin (1: 2,000, cat. no. 66009-1- Ig, Proteintech), COL1A1 (1: 2,000, cat. no. 67288-1-Ig, Proteintech), OCN (1: 2,000, cat. no. 20277-1-AP, Proteintech), RUNX2 (1: 2,000, cat. no. 20700-1-AP, Proteintech), C/EBPα (1: 2,000, cat. no. 29388-1-AP, Proteintech), FABP4 (1: 2,000, cat. no. 12802-1-AP, Proteintech), PPARγ (1: 2,000, cat. no. 16643-1-AP, Proteintech), ACAN (1: 2,000, cat. no. 68350-1-Ig, Proteintech), COL2A1 (1: 2,000, cat. no. 28459-1-AP, Proteintech), SOX9 (1: 2,000, cat. no. 67439-1-Ig, Proteintech), MAP2 (1: 2,000, cat. no. 17490-1-AP, Proteintech), Nestin (1: 2,000, cat. no. 19483-1-AP, Proteintech), TUBB3 (1: 2,000, cat. no. 66375-1-Ig, Proteintech), IFIT1 (1: 2,000, cat. no. ab305301, Abcam), IFIT2 (1: 2,000, cat. no. 12604-1-AP, Proteintech), IFIT3 (1: 2,000, cat. no. 15201-1-AP, Proteintech), eIF3C (1: 2,000, cat. no. 12733-1-AP, Proteintech), eIF3E (1: 2,000, cat. no. 10899-1-AP, Proteintech), RPS3 (1: 2,000, cat. no. 66046-1-Ig, Proteintech), SPP1 (1: 2,000, cat. no. 22952-1-AP, Proteintech), FGF18 (1: 2,000, cat. no. 11495-1-AP, Proteintech), MMP14 (1: 2,000, cat. no. 14552-1-AP, Proteintech), BMP2 (1: 2,000, cat. no. 66383-1-Ig, Proteintech), horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1: 5,000, cat. no. SA00001-2, Proteintech), and HRP-conjugated goat anti-mouse IgG (1: 5,000, cat. no. SA00001-1, Proteintech). ..

    other:

    Article Title: Multifunctional Hydrophilic Selenium‐Polyurea for Controlled Ion Release of Biodegradable Alloys and Immuno‐osteogenic Synergistic Modulation
    Article Snippet: Early burst ion release of biodegradable alloys, such as Zn2+, often triggers inflammation and impairs bone formation, which affects the clinical application of biodegradable alloys.. Here, a multifunctional hydrophilic selenium-containing polyurea (SePUA) is designed with a tunable ratio of selenium-containing groups to hydrophilic groups.. The SePUA can form stable Se-metal coordination, achieving controlled ion release of biodegradable alloys, and simultaneously leverages the biological functions of selenium to realize immuno-osteogenic synergistic modulation.

    Immunocytochemistry:

    Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
    Article Snippet: The PrimeScript RT reagent Kit with gDNA Eraser was purchased from Takara (Japan), and SYBR Green PCR Master Mix was purchased from Monad (China). .. Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China). .. The IL-6, SP7, BMP2, and COL1A1 antibody was purchased from Abcam (UK).

    Immunofluorescence:

    Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
    Article Snippet: The PrimeScript RT reagent Kit with gDNA Eraser was purchased from Takara (Japan), and SYBR Green PCR Master Mix was purchased from Monad (China). .. Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China). .. The IL-6, SP7, BMP2, and COL1A1 antibody was purchased from Abcam (UK).

    Staining:

    Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
    Article Snippet: The PrimeScript RT reagent Kit with gDNA Eraser was purchased from Takara (Japan), and SYBR Green PCR Master Mix was purchased from Monad (China). .. Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China). .. The IL-6, SP7, BMP2, and COL1A1 antibody was purchased from Abcam (UK).



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    Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) <t>Immunofluorescence</t> staining of <t>OCN</t> and OPN in BO after the different treatments. H) Western blot of RUNX2 and <t>OCN</t> expression. I,J) Quantification of RUNX2 and <t>OCN.</t> Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
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    Image Search Results


    Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) Immunofluorescence staining of OCN and OPN in BO after the different treatments. H) Western blot of RUNX2 and OCN expression. I,J) Quantification of RUNX2 and OCN. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Journal: Materials Today Bio

    Article Title: Hydrogel-integrated osteogenic microtissues promote repair of infected intervertebral defects through sequential immunomodulation

    doi: 10.1016/j.mtbio.2026.103657

    Figure Lengend Snippet: Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) Immunofluorescence staining of OCN and OPN in BO after the different treatments. H) Western blot of RUNX2 and OCN expression. I,J) Quantification of RUNX2 and OCN. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Article Snippet: Osteogenic protein expression in bone microtissues was assessed by immunofluorescence staining for OCN (ABclonal, A6205, 1:200) and OPN (ABclonal, A23658, 1:200) and by western blotting for RUNX2(Abcam, ab192256,1:1000), OCN (ABclonal, A6205, 1:1000), and GAPDH (ABclonal, AC001, 1:10,000).

    Techniques: Staining, Analysis, Activity Assay, Immunofluorescence, Western Blot, Expressing