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( A ) Representative image showing the inhibition of hypocotyl elongation in de-etiolated pifq and cop1-4 mutants grown in the dark or light and with or without ABA treatment. ( B ) Hypocotyl length measurements corresponding to the experiments shown in (A). Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media containing 10 μM ABA or mock for 4 days (dark-grown) or 5 days (light-grown). ( C ) Hypocotyl length of WT and pifq seedlings treated as described in (B) and transferred to a medium supplied with <t>mock,</t> <t>BZR,</t> or Paclo, with or without ABA (10 μM). ( D ) ABA repression of hypocotyl elongation in the light requires auxin transport. Hypocotyl length of WT and pifq seedlings grown under dark or low light conditions. Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media supplemented with ABA (10 μM) or <t>NPA</t> (0.5 μM) for 4 days (dark) or 5 days (light). Data are presented as mean ( n > 20) ± SD, with asterisks indicating significant differences from the mock control (* P < 0.05; ** P < 0.01) in one-way ANOVA with Tukey post hoc test.
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( A ) Representative image showing the inhibition of hypocotyl elongation in de-etiolated pifq and cop1-4 mutants grown in the dark or light and with or without ABA treatment. ( B ) Hypocotyl length measurements corresponding to the experiments shown in (A). Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media containing 10 μM ABA or mock for 4 days (dark-grown) or 5 days (light-grown). ( C ) Hypocotyl length of WT and pifq seedlings treated as described in (B) and transferred to a medium supplied with <t>mock,</t> <t>BZR,</t> or Paclo, with or without ABA (10 μM). ( D ) ABA repression of hypocotyl elongation in the light requires auxin transport. Hypocotyl length of WT and pifq seedlings grown under dark or low light conditions. Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media supplemented with ABA (10 μM) or <t>NPA</t> (0.5 μM) for 4 days (dark) or 5 days (light). Data are presented as mean ( n > 20) ± SD, with asterisks indicating significant differences from the mock control (* P < 0.05; ** P < 0.01) in one-way ANOVA with Tukey post hoc test.
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( A ) Representative image showing the inhibition of hypocotyl elongation in de-etiolated pifq and cop1-4 mutants grown in the dark or light and with or without ABA treatment. ( B ) Hypocotyl length measurements corresponding to the experiments shown in (A). Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media containing 10 μM ABA or mock for 4 days (dark-grown) or 5 days (light-grown). ( C ) Hypocotyl length of WT and pifq seedlings treated as described in (B) and transferred to a medium supplied with <t>mock,</t> <t>BZR,</t> or Paclo, with or without ABA (10 μM). ( D ) ABA repression of hypocotyl elongation in the light requires auxin transport. Hypocotyl length of WT and pifq seedlings grown under dark or low light conditions. Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media supplemented with ABA (10 μM) or <t>NPA</t> (0.5 μM) for 4 days (dark) or 5 days (light). Data are presented as mean ( n > 20) ± SD, with asterisks indicating significant differences from the mock control (* P < 0.05; ** P < 0.01) in one-way ANOVA with Tukey post hoc test.
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( A ) Representative image showing the inhibition of hypocotyl elongation in de-etiolated pifq and cop1-4 mutants grown in the dark or light and with or without ABA treatment. ( B ) Hypocotyl length measurements corresponding to the experiments shown in (A). Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media containing 10 μM ABA or mock for 4 days (dark-grown) or 5 days (light-grown). ( C ) Hypocotyl length of WT and pifq seedlings treated as described in (B) and transferred to a medium supplied with <t>mock,</t> <t>BZR,</t> or Paclo, with or without ABA (10 μM). ( D ) ABA repression of hypocotyl elongation in the light requires auxin transport. Hypocotyl length of WT and pifq seedlings grown under dark or low light conditions. Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media supplemented with ABA (10 μM) or <t>NPA</t> (0.5 μM) for 4 days (dark) or 5 days (light). Data are presented as mean ( n > 20) ± SD, with asterisks indicating significant differences from the mock control (* P < 0.05; ** P < 0.01) in one-way ANOVA with Tukey post hoc test.
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( A ) Representative image showing the inhibition of hypocotyl elongation in de-etiolated pifq and cop1-4 mutants grown in the dark or light and with or without ABA treatment. ( B ) Hypocotyl length measurements corresponding to the experiments shown in (A). Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media containing 10 μM ABA or mock for 4 days (dark-grown) or 5 days (light-grown). ( C ) Hypocotyl length of WT and pifq seedlings treated as described in (B) and transferred to a medium supplied with <t>mock,</t> <t>BZR,</t> or Paclo, with or without ABA (10 μM). ( D ) ABA repression of hypocotyl elongation in the light requires auxin transport. Hypocotyl length of WT and pifq seedlings grown under dark or low light conditions. Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media supplemented with ABA (10 μM) or <t>NPA</t> (0.5 μM) for 4 days (dark) or 5 days (light). Data are presented as mean ( n > 20) ± SD, with asterisks indicating significant differences from the mock control (* P < 0.05; ** P < 0.01) in one-way ANOVA with Tukey post hoc test.
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( A ) Representative image showing the inhibition of hypocotyl elongation in de-etiolated pifq and cop1-4 mutants grown in the dark or light and with or without ABA treatment. ( B ) Hypocotyl length measurements corresponding to the experiments shown in (A). Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media containing 10 μM ABA or mock for 4 days (dark-grown) or 5 days (light-grown). ( C ) Hypocotyl length of WT and pifq seedlings treated as described in (B) and transferred to a medium supplied with mock, BZR, or Paclo, with or without ABA (10 μM). ( D ) ABA repression of hypocotyl elongation in the light requires auxin transport. Hypocotyl length of WT and pifq seedlings grown under dark or low light conditions. Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media supplemented with ABA (10 μM) or NPA (0.5 μM) for 4 days (dark) or 5 days (light). Data are presented as mean ( n > 20) ± SD, with asterisks indicating significant differences from the mock control (* P < 0.05; ** P < 0.01) in one-way ANOVA with Tukey post hoc test.

Journal: Science Advances

Article Title: A PIF-SAUR module safeguards hypocotyl elongation from ABA inhibition in the dark

doi: 10.1126/sciadv.adv0895

Figure Lengend Snippet: ( A ) Representative image showing the inhibition of hypocotyl elongation in de-etiolated pifq and cop1-4 mutants grown in the dark or light and with or without ABA treatment. ( B ) Hypocotyl length measurements corresponding to the experiments shown in (A). Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media containing 10 μM ABA or mock for 4 days (dark-grown) or 5 days (light-grown). ( C ) Hypocotyl length of WT and pifq seedlings treated as described in (B) and transferred to a medium supplied with mock, BZR, or Paclo, with or without ABA (10 μM). ( D ) ABA repression of hypocotyl elongation in the light requires auxin transport. Hypocotyl length of WT and pifq seedlings grown under dark or low light conditions. Seedlings were germinated in media without ABA for 62 hours (dark) or 72 hours (light) and transferred to media supplemented with ABA (10 μM) or NPA (0.5 μM) for 4 days (dark) or 5 days (light). Data are presented as mean ( n > 20) ± SD, with asterisks indicating significant differences from the mock control (* P < 0.05; ** P < 0.01) in one-way ANOVA with Tukey post hoc test.

Article Snippet: For the hormone inhibitors, 0.5 μM NPA (Duchefa Biochemie), 0.5 μM BZR (MedChemExpress), and 0.1 μM Paclo (Duchefa Biochemie) were used.

Techniques: Inhibition, Control