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  • 93
    Millipore npa
    Application of <t>IAA</t> reduces PR growth and increases LR density, whereas <t>NPA</t> treatment decreases LR density in wild-type (WT) and siz1 seedlings. Seedlings were grown on basal medium containing Pi for 3.5 d before being transferred onto medium containing
    Npa, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa/product/Millipore
    Average 93 stars, based on 204 article reviews
    Price from $9.99 to $1999.99
    npa - by Bioz Stars, 2020-05
    93/100 stars
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    88
    Millipore 3 npa
    Effect of oxidative stress on granulosa cell apoptosis. Ovaries were collected after <t>3-NPA</t> different doses treatment for 7 days (A) TUNEL staining of ovarian paraffin sections. Scale bars are 100 µm. (B) The percentage of TUNEL-positive follicles. (C) The relative expression levels of Bcl-2 and Bax in granulosa cells. Data show the means ± SEM (n = 5). * P
    3 Npa, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    3 npa - by Bioz Stars, 2020-05
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    88
    Millipore 4 npa
    Effect of oxidative stress on granulosa cell apoptosis. Ovaries were collected after <t>3-NPA</t> different doses treatment for 7 days (A) TUNEL staining of ovarian paraffin sections. Scale bars are 100 µm. (B) The percentage of TUNEL-positive follicles. (C) The relative expression levels of Bcl-2 and Bax in granulosa cells. Data show the means ± SEM (n = 5). * P
    4 Npa, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Tocris l npa
    Effect of oxidative stress on granulosa cell apoptosis. Ovaries were collected after <t>3-NPA</t> different doses treatment for 7 days (A) TUNEL staining of ovarian paraffin sections. Scale bars are 100 µm. (B) The percentage of TUNEL-positive follicles. (C) The relative expression levels of Bcl-2 and Bax in granulosa cells. Data show the means ± SEM (n = 5). * P
    L Npa, supplied by Tocris, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    l npa - by Bioz Stars, 2020-05
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    93
    Microtrac nanotrac npa 250
    Effect of oxidative stress on granulosa cell apoptosis. Ovaries were collected after <t>3-NPA</t> different doses treatment for 7 days (A) TUNEL staining of ovarian paraffin sections. Scale bars are 100 µm. (B) The percentage of TUNEL-positive follicles. (C) The relative expression levels of Bcl-2 and Bax in granulosa cells. Data show the means ± SEM (n = 5). * P
    Nanotrac Npa 250, supplied by Microtrac, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nanotrac npa 250 - by Bioz Stars, 2020-05
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    92
    Chem Service Inc npa
    Effect of oxidative stress on granulosa cell apoptosis. Ovaries were collected after <t>3-NPA</t> different doses treatment for 7 days (A) TUNEL staining of ovarian paraffin sections. Scale bars are 100 µm. (B) The percentage of TUNEL-positive follicles. (C) The relative expression levels of Bcl-2 and Bax in granulosa cells. Data show the means ± SEM (n = 5). * P
    Npa, supplied by Chem Service Inc, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa/product/Chem Service Inc
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    npa  (Duchefa)
    92
    Duchefa npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Duchefa, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    npa - by Bioz Stars, 2020-05
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    npa  (Tocris)
    92
    Tocris npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Tokyo Chemical Industry npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Medline Industries npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Medline Industries, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa/product/Medline Industries
    Average 92 stars, based on 1 article reviews
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    92
    Frinton Laboratories npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Frinton Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa/product/Frinton Laboratories
    Average 92 stars, based on 1 article reviews
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    92/100 stars
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    npa  (Mednet)
    93
    Mednet npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Mednet, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    npa  (Supelco)
    92
    Supelco npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Supelco, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa/product/Supelco
    Average 92 stars, based on 3 article reviews
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    92
    Becton Dickinson npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 16 article reviews
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    92
    Johnson & Johnson npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa/product/Johnson & Johnson
    Average 92 stars, based on 2 article reviews
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    92
    OlChemIm npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by OlChemIm, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa/product/OlChemIm
    Average 92 stars, based on 2 article reviews
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    91
    Hologic Inc npa
    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m <t>NPA</t> (A and C) and then transferred 4 d on medium supplemented with 10 −5 m <t>NAA</t> (B and D). Asterisks indicate lateral
    Npa, supplied by Hologic Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa/product/Hologic Inc
    Average 91 stars, based on 1 article reviews
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    92
    Supelco 1 naphthylphthalamic acid npa
    Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; <t>NPA,</t> N <t>-1-naphthylphthalamic</t> acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.
    1 Naphthylphthalamic Acid Npa, supplied by Supelco, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 naphthylphthalamic acid npa/product/Supelco
    Average 92 stars, based on 4 article reviews
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    1 naphthylphthalamic acid npa - by Bioz Stars, 2020-05
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    85
    Thermo Fisher multi npa kit
    Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; <t>NPA,</t> N <t>-1-naphthylphthalamic</t> acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.
    Multi Npa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Janssen zuithoff npa
    Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; <t>NPA,</t> N <t>-1-naphthylphthalamic</t> acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.
    Zuithoff Npa, supplied by Janssen, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zuithoff npa/product/Janssen
    Average 86 stars, based on 5 article reviews
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    zuithoff npa - by Bioz Stars, 2020-05
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    85
    Millipore ρ npa
    Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; <t>NPA,</t> N <t>-1-naphthylphthalamic</t> acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.
    ρ Npa, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ρ npa/product/Millipore
    Average 85 stars, based on 1 article reviews
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    86
    Oxford Nanopore npa arrays
    Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; <t>NPA,</t> N <t>-1-naphthylphthalamic</t> acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.
    Npa Arrays, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    IDEXX npa values
    Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; <t>NPA,</t> N <t>-1-naphthylphthalamic</t> acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.
    Npa Values, supplied by IDEXX, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npa values/product/IDEXX
    Average 92 stars, based on 2 article reviews
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    npa values - by Bioz Stars, 2020-05
    92/100 stars
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    85
    Enzo Biochem l npa
    Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; <t>NPA,</t> N <t>-1-naphthylphthalamic</t> acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.
    L Npa, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    Image Search Results


    Application of IAA reduces PR growth and increases LR density, whereas NPA treatment decreases LR density in wild-type (WT) and siz1 seedlings. Seedlings were grown on basal medium containing Pi for 3.5 d before being transferred onto medium containing

    Journal: Plant Physiology

    Article Title: SIZ1 Regulation of Phosphate Starvation-Induced Root Architecture Remodeling Involves the Control of Auxin Accumulation 1 Regulation of Phosphate Starvation-Induced Root Architecture Remodeling Involves the Control of Auxin Accumulation 1 [C] Regulation of Phosphate Starvation-Induced Root Architecture Remodeling Involves the Control of Auxin Accumulation 1 [C] [W] Regulation of Phosphate Starvation-Induced Root Architecture Remodeling Involves the Control of Auxin Accumulation 1 [C] [W] [OA]

    doi: 10.1104/pp.110.165191

    Figure Lengend Snippet: Application of IAA reduces PR growth and increases LR density, whereas NPA treatment decreases LR density in wild-type (WT) and siz1 seedlings. Seedlings were grown on basal medium containing Pi for 3.5 d before being transferred onto medium containing

    Article Snippet: IAA and NPA were purchased from Sigma Chemicals.

    Techniques:

    Changes in α - l -arafase activities, accumulation of protein, and expression of mRNA during fruit development and ripening. A, Activity of α - l -arafase solubilized from the cell wall using p NPA as substrate. The activity on a per gram fresh

    Journal:

    Article Title: Isolation, Characterization, and Cloning of α-l-Arabinofuranosidase Expressed during Fruit Ripening of Japanese Pear

    doi: 10.1104/pp.104.056655

    Figure Lengend Snippet: Changes in α - l -arafase activities, accumulation of protein, and expression of mRNA during fruit development and ripening. A, Activity of α - l -arafase solubilized from the cell wall using p NPA as substrate. The activity on a per gram fresh

    Article Snippet: Assay of α - l -arafase activity using p NPA (Sigma, St. Louis) was performed according to the method described previously ( ).

    Techniques: Expressing, Activity Assay

    Effect of oxidative stress on granulosa cell apoptosis. Ovaries were collected after 3-NPA different doses treatment for 7 days (A) TUNEL staining of ovarian paraffin sections. Scale bars are 100 µm. (B) The percentage of TUNEL-positive follicles. (C) The relative expression levels of Bcl-2 and Bax in granulosa cells. Data show the means ± SEM (n = 5). * P

    Journal: PLoS ONE

    Article Title: 3-Nitropropionic Acid Induces Ovarian Oxidative Stress and Impairs Follicle in Mouse

    doi: 10.1371/journal.pone.0086589

    Figure Lengend Snippet: Effect of oxidative stress on granulosa cell apoptosis. Ovaries were collected after 3-NPA different doses treatment for 7 days (A) TUNEL staining of ovarian paraffin sections. Scale bars are 100 µm. (B) The percentage of TUNEL-positive follicles. (C) The relative expression levels of Bcl-2 and Bax in granulosa cells. Data show the means ± SEM (n = 5). * P

    Article Snippet: For injection, 3-NPA (Sigma, Location) was dissolved in normal saline and the pH was adjusted to 7.4 with sodium hydroxide.

    Techniques: TUNEL Assay, Staining, Expressing

    Serum levels of reproductive hormones in 3-NPA-treated mice. The serum levels of estradiol (E2) (A), progesterone (P4) (B), follicle-stimulating hormone (FSH) (C) and luteinizing hormone (LH) (D) were determined by ELISA in control and 3-NPA-treated mice. Data show the means ± SEM (n = 10). *P

    Journal: PLoS ONE

    Article Title: 3-Nitropropionic Acid Induces Ovarian Oxidative Stress and Impairs Follicle in Mouse

    doi: 10.1371/journal.pone.0086589

    Figure Lengend Snippet: Serum levels of reproductive hormones in 3-NPA-treated mice. The serum levels of estradiol (E2) (A), progesterone (P4) (B), follicle-stimulating hormone (FSH) (C) and luteinizing hormone (LH) (D) were determined by ELISA in control and 3-NPA-treated mice. Data show the means ± SEM (n = 10). *P

    Article Snippet: For injection, 3-NPA (Sigma, Location) was dissolved in normal saline and the pH was adjusted to 7.4 with sodium hydroxide.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Effect of 3-NPA treatment over different time periods on ROS levels in different tissues. (A) Mice were intraperitoneally injected with saline versus 3-NPA (12.5 mg/kg) for 7 days. ROS levels in granulosa cells and other tissues were quantified by nitro blue tetrazolium (NBT) staining. (B and C) Mice were intraperitoneally injected with saline or 3-NPA (12.5 mg/kg) for 3 and 7 days, respectively. ROS levels in granulosa cells and oocytes were detected by dihydroethidium bromide fluorescence (red), and nuclei were counterstained with DAPI (blue). (D and E) ROS levels in granulosa cells were quantified by nitro blue tetrazolium (NBT) staining. Scale bars are 100 µm. Bars represent means ± SEM (n = 5); *significant difference from control, P

    Journal: PLoS ONE

    Article Title: 3-Nitropropionic Acid Induces Ovarian Oxidative Stress and Impairs Follicle in Mouse

    doi: 10.1371/journal.pone.0086589

    Figure Lengend Snippet: Effect of 3-NPA treatment over different time periods on ROS levels in different tissues. (A) Mice were intraperitoneally injected with saline versus 3-NPA (12.5 mg/kg) for 7 days. ROS levels in granulosa cells and other tissues were quantified by nitro blue tetrazolium (NBT) staining. (B and C) Mice were intraperitoneally injected with saline or 3-NPA (12.5 mg/kg) for 3 and 7 days, respectively. ROS levels in granulosa cells and oocytes were detected by dihydroethidium bromide fluorescence (red), and nuclei were counterstained with DAPI (blue). (D and E) ROS levels in granulosa cells were quantified by nitro blue tetrazolium (NBT) staining. Scale bars are 100 µm. Bars represent means ± SEM (n = 5); *significant difference from control, P

    Article Snippet: For injection, 3-NPA (Sigma, Location) was dissolved in normal saline and the pH was adjusted to 7.4 with sodium hydroxide.

    Techniques: Mouse Assay, Injection, Staining, Fluorescence

    Effect of 3-NPA injection for 7 days on antioxidant enzymes activities and mRNA levels in different organ tissues. Mice were intraperitoneally injected with saline versus 3-NPA (12.5 mg/kg) for 7 days. (A) Antioxidant enzymes activities. (B) Relative expression levels of SOD2, GP X and CAT. Data show the means ± SEM (n = 5). * P

    Journal: PLoS ONE

    Article Title: 3-Nitropropionic Acid Induces Ovarian Oxidative Stress and Impairs Follicle in Mouse

    doi: 10.1371/journal.pone.0086589

    Figure Lengend Snippet: Effect of 3-NPA injection for 7 days on antioxidant enzymes activities and mRNA levels in different organ tissues. Mice were intraperitoneally injected with saline versus 3-NPA (12.5 mg/kg) for 7 days. (A) Antioxidant enzymes activities. (B) Relative expression levels of SOD2, GP X and CAT. Data show the means ± SEM (n = 5). * P

    Article Snippet: For injection, 3-NPA (Sigma, Location) was dissolved in normal saline and the pH was adjusted to 7.4 with sodium hydroxide.

    Techniques: Injection, Mouse Assay, Expressing

    Effects of 3-NPA treatment on fetus/embryo development in vivo. Female mice were intraperitoneally injected with saline or oxidant 3-NPA for 7 days, respectively. On GD7.5, GD12.5 and GD18, the pregnant mice (n = 5, each group) were sacrificed and mouse embryos were isolated. The growth development of embryos was observed.

    Journal: PLoS ONE

    Article Title: 3-Nitropropionic Acid Induces Ovarian Oxidative Stress and Impairs Follicle in Mouse

    doi: 10.1371/journal.pone.0086589

    Figure Lengend Snippet: Effects of 3-NPA treatment on fetus/embryo development in vivo. Female mice were intraperitoneally injected with saline or oxidant 3-NPA for 7 days, respectively. On GD7.5, GD12.5 and GD18, the pregnant mice (n = 5, each group) were sacrificed and mouse embryos were isolated. The growth development of embryos was observed.

    Article Snippet: For injection, 3-NPA (Sigma, Location) was dissolved in normal saline and the pH was adjusted to 7.4 with sodium hydroxide.

    Techniques: In Vivo, Mouse Assay, Injection, Isolation

    Effect of 3-NPA treatment at varying doses for 7 days on weight gain, body and organ weights. (A) Weight gain and (B) Body weight among groups. (C) Ovary, (D) Liver, (E) kidney and (F) spleen indices after 3-NPA injection. Bars represent means ± SEM (n = 10). * Significant difference from control, P

    Journal: PLoS ONE

    Article Title: 3-Nitropropionic Acid Induces Ovarian Oxidative Stress and Impairs Follicle in Mouse

    doi: 10.1371/journal.pone.0086589

    Figure Lengend Snippet: Effect of 3-NPA treatment at varying doses for 7 days on weight gain, body and organ weights. (A) Weight gain and (B) Body weight among groups. (C) Ovary, (D) Liver, (E) kidney and (F) spleen indices after 3-NPA injection. Bars represent means ± SEM (n = 10). * Significant difference from control, P

    Article Snippet: For injection, 3-NPA (Sigma, Location) was dissolved in normal saline and the pH was adjusted to 7.4 with sodium hydroxide.

    Techniques: Injection

    Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m NPA (A and C) and then transferred 4 d on medium supplemented with 10 −5 m NAA (B and D). Asterisks indicate lateral

    Journal:

    Article Title: Diarch Symmetry of the Vascular Bundle in Arabidopsis Root Encompasses the Pericycle and Is Reflected in Distich Lateral Root Initiation 1Diarch Symmetry of the Vascular Bundle in Arabidopsis Root Encompasses the Pericycle and Is Reflected in Distich Lateral Root Initiation 1 [W]

    doi: 10.1104/pp.107.107870

    Figure Lengend Snippet: Lateral root initiation in lhw mutant. J0121 (A and B) and lhw (C and D) plants 3 DAG on medium supplemented with 10 −5 m NPA (A and C) and then transferred 4 d on medium supplemented with 10 −5 m NAA (B and D). Asterisks indicate lateral

    Article Snippet: For treatments with NPA (Duchefa) or NAA (Sigma-Aldrich), 10 μ m was added to the Murashige and Skoog medium ( ).

    Techniques: Mutagenesis

    Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; NPA, N -1-naphthylphthalamic acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.

    Journal: Frontiers in Plant Science

    Article Title: Evidence for Brassinosteroid-Mediated PAT During Germination of Spathoglottis plicata (Orchidaceae)

    doi: 10.3389/fpls.2018.01215

    Figure Lengend Snippet: Seedling morphology for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; TIBA, 2,3,5-Triiodobenzoic acid; NPA, N -1-naphthylphthalamic acid. P, protocorm; EP, elongated protocorm; FL, first leaf; LFL, lobed first leaf; CFL, callused first leaf; FFL, fleshy first leaf; PH, protocorm hairs. Scale bar = 400 μm.

    Article Snippet: For the PAT inhibitor experiments, brefeldin A (BFA) (Sigma-Aldrich Chemical), 2,3,5-Triiodobenzoic acid (TIBA) (Sigma-Aldrich Chemical), N -1-naphthylphthalamic acid (NPA) (Supelco, Bellefonte, PA, United States), or monensin (MSN) (MP Biomedicals LLC, Solon, OH, United States) were added to culture media with a final concentration of 30 μM for BFA and 100 μM for the other three PAT inhibitors.

    Techniques: Cell Culture

    Growth parameters (A) , percentage of seedlings with first leaf (B) , and percentage of seedlings with a fleshy, callused, or lobed first leaf (C) for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; 2,4-D, 2,4-dichlorophenoxyacetic acid; NPA, N -1-naphthylphthalamic acid; TIBA, 2,3,5-Triiodobenzoic acid; BFA, brefeldin A; FLL, first leaf length; PL, protocorm length. Growth parameter data are presented as means ± standard deviation ( n = 80, except as indicated in materials and methods). Letters indicate shared significance groups for each growth parameter for different treatments (Tukey’s HSD) at p ≤ 0.05. Percentage data are presented as means ± standard deviation ( n = 150, three groups of 50). Asterisks indicate a significant increase in overall shoot growth (A) , percentage with first leaf (B) , or a decrease in percentage with abnormal leaves (C) when eBL is added to the treatment. ∗ Significance at p ≤ 0.05.

    Journal: Frontiers in Plant Science

    Article Title: Evidence for Brassinosteroid-Mediated PAT During Germination of Spathoglottis plicata (Orchidaceae)

    doi: 10.3389/fpls.2018.01215

    Figure Lengend Snippet: Growth parameters (A) , percentage of seedlings with first leaf (B) , and percentage of seedlings with a fleshy, callused, or lobed first leaf (C) for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; 2,4-D, 2,4-dichlorophenoxyacetic acid; NPA, N -1-naphthylphthalamic acid; TIBA, 2,3,5-Triiodobenzoic acid; BFA, brefeldin A; FLL, first leaf length; PL, protocorm length. Growth parameter data are presented as means ± standard deviation ( n = 80, except as indicated in materials and methods). Letters indicate shared significance groups for each growth parameter for different treatments (Tukey’s HSD) at p ≤ 0.05. Percentage data are presented as means ± standard deviation ( n = 150, three groups of 50). Asterisks indicate a significant increase in overall shoot growth (A) , percentage with first leaf (B) , or a decrease in percentage with abnormal leaves (C) when eBL is added to the treatment. ∗ Significance at p ≤ 0.05.

    Article Snippet: For the PAT inhibitor experiments, brefeldin A (BFA) (Sigma-Aldrich Chemical), 2,3,5-Triiodobenzoic acid (TIBA) (Sigma-Aldrich Chemical), N -1-naphthylphthalamic acid (NPA) (Supelco, Bellefonte, PA, United States), or monensin (MSN) (MP Biomedicals LLC, Solon, OH, United States) were added to culture media with a final concentration of 30 μM for BFA and 100 μM for the other three PAT inhibitors.

    Techniques: Cell Culture, Standard Deviation