Journal: Frontiers in Plant Science
Article Title: Evidence for Brassinosteroid-Mediated PAT During Germination of Spathoglottis plicata (Orchidaceae)
Figure Lengend Snippet: Growth parameters (A) , percentage of seedlings with first leaf (B) , and percentage of seedlings with a fleshy, callused, or lobed first leaf (C) for 10 DAC protocorms cultured with PAT inhibitors or growth inhibiting levels of auxin, with or without application of epibrassinolide (eBL) for an additional 15 days (25 DAC). NAA, 1-naphthaleneacetic acid; IAA, indoleacetic acid; 2,4-D, 2,4-dichlorophenoxyacetic acid; NPA, N -1-naphthylphthalamic acid; TIBA, 2,3,5-Triiodobenzoic acid; BFA, brefeldin A; FLL, first leaf length; PL, protocorm length. Growth parameter data are presented as means ± standard deviation ( n = 80, except as indicated in materials and methods). Letters indicate shared significance groups for each growth parameter for different treatments (Tukey’s HSD) at p ≤ 0.05. Percentage data are presented as means ± standard deviation ( n = 150, three groups of 50). Asterisks indicate a significant increase in overall shoot growth (A) , percentage with first leaf (B) , or a decrease in percentage with abnormal leaves (C) when eBL is added to the treatment. ∗ Significance at p ≤ 0.05.
Article Snippet: For the PAT inhibitor experiments, brefeldin A (BFA) (Sigma-Aldrich Chemical), 2,3,5-Triiodobenzoic acid (TIBA) (Sigma-Aldrich Chemical), N -1-naphthylphthalamic acid (NPA) (Supelco, Bellefonte, PA, United States), or monensin (MSN) (MP Biomedicals LLC, Solon, OH, United States) were added to culture media with a final concentration of 30 μM for BFA and 100 μM for the other three PAT inhibitors.
Techniques: Cell Culture, Standard Deviation