normocintm (InvivoGen)
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InvivoGen
normocintm
Normocintm, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 2615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normocintm/Normocin/pm42152018-58-66-70
Average 99 stars, based on 2615 article reviews
Normocintm, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 2615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normocintm/Normocin/pm42152018-58-66-70
Average 99 stars, based on 2615 article reviews
normocintm - by Bioz Stars,
2026-09
99/100 stars
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Cell Culture:Article Title: TLR/NF-κB activation in vitro as a tool in the assessment of occupational bioaerosol exposure and health risks in wastewater treatment plants. Article Snippet: .. HEK-BlueTM Null1, hTLR2, hTLR3, hTLR4, and hTLR7 cells (Invivogen, US) were cultured according to the manufacturer’s protocols at 37 °C and 5% CO2 in DMEM with GlutaMAXTM (GibcoTM/Thermo Fischer, US), 10% heat-inactivated ultra-low endotoxin FBS (BioWest, France), and 100 μg/mL Article Title: Serum amyloid A1 directly engages TLR4/MD-2 to trigger neuroinflammatory signaling in microglia cultures. Article Snippet: HEK-BlueTM hTLR4 cells, co-transfected with human TLR4, MD-2, and cluster of differentiation 14 (CD14) co-receptor genes, as well as NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene in human embryonic kidney 293 (HEK293) cells, were purchased from InvivoGen. .. Cells were cultured in high glucose DMEM supplemented with 2 mM L-glutamine, 10% FBS, 100 units/mL penicillin, 100 μg/mL streptomycin, 100 μg/mL Article Title: Proinflammatory Cytokine Preconditioning Enhances the Therapeutic Potency of Different Types of MSCs in Inflammation. Article Snippet: THP1-BlueTM-CD14 Cell Line THP1-BlueTM-CD14 cells (Cat# thp-cd14sp; InvivoGen, Toulouse, France) are derived from the human acute monocytic leukaemia cell line (THP-1) with stable transfection of a reporter plasmid expressing a secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by the transcription factors NF-κB and AP1. .. THP1-BlueTM-CD14 cells were used within passage 20 and cultured in Roswell Park Memorial Institute (RPMI) growth medium with L-glutamine (Cat# R8758; Sigma-Aldrich Ireland) supplemented with 10% FBS, 1% P/S and 100 μg/mL Article Title: Extracellular vesicles derived from Enterococcus faecalis: inflammatory activation does not require internalization. Article Snippet: HMDMs were seeded and differentiated in RPMI 1640 medium (cat. no. R0883, Sigma-Aldrich) containing 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% FCS, 100 U/ mL-100 μg/mL Pen-Strep, and 20 ng/mL human recombinant macrophage colony-stimulating factor (M-CSF, cat. no. 130-096-492, Miltenyi) for 6 days before their use [42]. .. cells NF-κB/AP-1 reporter monocytes, THP1-XBlueTM cells (cat.no. thpx-sp, Invivogen), derived from the human monocytic THP-1 cell line by transfection of a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an NF-κB- and AP-1-inducible promoter, were cultured in RPMI 1640 medium containing 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% heat-inactivated FCS, 100 mg/mL Article Title: Improved Systemic Immunochemotherapy Employing an Oxaliplatin-TLR7/8 Agonist Prodrug Strategy. Article Snippet: CT-26 cells were cultured in Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 (DMEM/F12, 1:1) (SAFC Biosciences Ltd., UK), MC-38 cells in DMEM, supplemented with NEAA (0.1 mM) (Thermo Fisher Scientific), sodium pyruvate (1 mM) and L-glutamine (2 mM), HCT116 and HCT116/OxR cells in McCoy’s 5A modified medium (M8403), and SW480 cells in Minimum Essential Medium Eagle (MEM; M0268). .. HEK-Blue/hTLR7 cells were cultured in DMEM, supplemented with L-glutamine (2 mM), Selection:Article Title: Serum amyloid A1 directly engages TLR4/MD-2 to trigger neuroinflammatory signaling in microglia cultures. Article Snippet: HEK-BlueTM hTLR4 cells, co-transfected with human TLR4, MD-2, and cluster of differentiation 14 (CD14) co-receptor genes, as well as NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene in human embryonic kidney 293 (HEK293) cells, were purchased from InvivoGen. .. Cells were cultured in high glucose DMEM supplemented with 2 mM L-glutamine, 10% FBS, 100 units/mL penicillin, 100 μg/mL streptomycin, 100 μg/mL Article Title: From Sea to Cell: Ascophyllum nodosum and Fucus vesiculosus Extracts Attenuate NF-κB-Mediated Inflammation and Protect Intestinal Barrier Integrity—A Comprehensive Analysis Applying In Vitro and In Vivo Models Article Snippet: .. The culture medium was supplemented with 10% heat-inactivated FBS, penicillin–streptomycin (100 U/mL-100 μg/mL), 100 μg/mL Modification:Article Title: Berry kefirs as functional non-dairy beverages: Linking microbial activity, phenolic profiles, and immunomodulatory properties Article Snippet: The human intestinal epithelial reporter cell line, HCT116-DualTM, was purchased from InvivoGen (Toulouse, France). .. Cells were grown in Dulbecco's Modified Eagle's Medium (Sigma-Aldrich, Missouri, USA), supplemented with 10 % fetal bovine serum, and 1 % penicillin/streptomycin (Sigma-Aldrich), 10 μg/mL of blasticidin, 100 μg/mL Zeocin®, and 100 μg/mL of Derivative Assay:Article Title: Extracellular vesicles derived from Enterococcus faecalis: inflammatory activation does not require internalization. Article Snippet: HMDMs were seeded and differentiated in RPMI 1640 medium (cat. no. R0883, Sigma-Aldrich) containing 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% FCS, 100 U/ mL-100 μg/mL Pen-Strep, and 20 ng/mL human recombinant macrophage colony-stimulating factor (M-CSF, cat. no. 130-096-492, Miltenyi) for 6 days before their use [42]. .. cells NF-κB/AP-1 reporter monocytes, THP1-XBlueTM cells (cat.no. thpx-sp, Invivogen), derived from the human monocytic THP-1 cell line by transfection of a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an NF-κB- and AP-1-inducible promoter, were cultured in RPMI 1640 medium containing 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% heat-inactivated FCS, 100 mg/mL Transfection:Article Title: Extracellular vesicles derived from Enterococcus faecalis: inflammatory activation does not require internalization. Article Snippet: HMDMs were seeded and differentiated in RPMI 1640 medium (cat. no. R0883, Sigma-Aldrich) containing 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% FCS, 100 U/ mL-100 μg/mL Pen-Strep, and 20 ng/mL human recombinant macrophage colony-stimulating factor (M-CSF, cat. no. 130-096-492, Miltenyi) for 6 days before their use [42]. .. cells NF-κB/AP-1 reporter monocytes, THP1-XBlueTM cells (cat.no. thpx-sp, Invivogen), derived from the human monocytic THP-1 cell line by transfection of a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an NF-κB- and AP-1-inducible promoter, were cultured in RPMI 1640 medium containing 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% heat-inactivated FCS, 100 mg/mL Control:Article Title: Extracellular vesicles derived from Enterococcus faecalis: inflammatory activation does not require internalization. Article Snippet: HMDMs were seeded and differentiated in RPMI 1640 medium (cat. no. R0883, Sigma-Aldrich) containing 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% FCS, 100 U/ mL-100 μg/mL Pen-Strep, and 20 ng/mL human recombinant macrophage colony-stimulating factor (M-CSF, cat. no. 130-096-492, Miltenyi) for 6 days before their use [42]. .. cells NF-κB/AP-1 reporter monocytes, THP1-XBlueTM cells (cat.no. thpx-sp, Invivogen), derived from the human monocytic THP-1 cell line by transfection of a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an NF-κB- and AP-1-inducible promoter, were cultured in RPMI 1640 medium containing 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% heat-inactivated FCS, 100 mg/mL |