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nitrocellulose membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad nitrocellulose membrane
    Nitrocellulose Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 32487 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrocellulose/Nitrocellulose+Membrane/10__22175_slash_mmb__17646-66-22-24
    Average 99 stars, based on 32487 article reviews
    nitrocellulose membrane - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    SDS Page:

    Article Title: An integrative approach to studying sphingolipid metabolism reveals p53 as a master regulator of the pathway.
    Article Snippet: Lysates were diluted with Bio-Rad's 2x Laemmli buffer and boiled for 5-7 minutes. .. Equal amounts of proteins (10-20 g of total lysate) were separated by SDS-PAGE on Criterion gels (Thermo Fisher Scientific), transferred onto nitrocellulose (Bio-Rad, Cat# 1620115). .. After blocking with 5% nonfat milk in 0.1% Tween PBS for 2 h at room temperature, CERT (Novus Biologicals, Rabbit pAb, B100-2114, 1:2500 in 2% BSA) and Actin (Santa Cruz Biotechnology I-19, Goat pAb, sc-1616, 1:2000 in 5% BSA) primary antibodies were incubated overnight at 4°C.

    Article Title: Coronaviruses reprogram the tRNA epitranscriptome to favor viral protein expression
    Article Snippet: .. Samples were separated on denaturing SDS-PAGE gels and transferred electrophoretically onto nitrocellulose (trans-blot turbo, BioRad 12023835) by turbo-blotting (trans-blot turbo transfer system, BioRad 1704150EDU). .. Antibodies recognizing the following epitopes and proteins were used: SARS-CoV-2 NSP8 (GeneTex GTX632696, 1:1000); KIAA1456 (Thermo Fisher PA4-70320, 1:500); ALKBH8 (AV41106Sigma-Aldrich AV41106, 1:1000); ADAT2 (mybiosurce mbs2621771, 1:1000); ALKBH1 (Abcam ab126596, 1:1000); NSUN2 (Proteintech 20854-1-AP, 1:5000); QTRT1 (D7) (Santa Cruz Biotechnology sc398918, 1:1000); Vinculin (Sigma-Aldrich V9131, 1:1000); GAPDH (Proteintech 60004-1-IG, 1:10000); Phospho-histone H2AX (Ser139) (Cell Signaling 9718S, 1:1000); ATM (D2E2) (Cell Signaling 2873S, 1:1000); Phospho-ATM (S1981) (Cell Signaling 13050S, 1:1000); ATR (Cell Signaling 2790S, 1:1000); Phospho-ATR (S428) (Cell Signaling 2853S, 1:1000); Phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling 9215T, 1:1000); SLFN11 (E4) (Santa Cruz Biotechnology sc374339, 1:1000); SLFN12 (Abmart X-C9J4K7-N, 1:1000); HCoV-OC43 Nucleocapsid Antibody (NP) (SinoBiological -abyntech 40643-T62, 1:20000); ELP3 (D5H12) (Cell Signaling 5728, 1:1000).

    Article Title: An integrative approach to studying sphingolipid metabolism reveals p53 as a master regulator of the pathway
    Article Snippet: Lysates were diluted with Bio-Rad's 2x Laemmli buffer and boiled for 5–7 min. .. Equal amounts of proteins (10–20 μg of total lysate) were separated by SDS-PAGE on Criterion gels (Thermo Fisher Scientific) and transferred onto nitrocellulose (Bio-Rad, catalog no.: 1620115). .. After blocking with 5% nonfat milk in 0.1% Tween PBS for 2 h at room temperature, CERT (Novus Biologicals, Rabbit pAb, B100-2114, 1:2,500 in 2% BSA) and actin (Santa Cruz Biotechnology I-19, Goat pAb, sc-1616, 1:2,000 in 5% BSA) primary antibodies were incubated overnight at 4°C.

    Article Title: Alternative organelle targeting of OPA1 mediates fatty acid release from lipid droplets
    Article Snippet: Protein concentration of subcellular fractions and clear lysates were quantified using the Pierce BCA Protein Assay (Thermo Fisher Scientific). .. Equal amount of protein was denatured in 1x SDS-loading buffer at 95°C for 5–10 min and was loaded into an SDS-PAGE protein gel before being transferred to a nitrocellulose (Bio-Rad) or PVDF (Bio-Rad) membrane for western blotting. .. For western blotting, membranes were blocked with EveryBlot blocking buffer (Bio-Rad) for 1 h before being incubated with a primary antibody at room temperature for 1 h or at 4°C for overnight.

    Blocking Assay:

    Article Title: Multi-omic phenotyping of MAPT V337M neurons reveals early changes in axonogenesis and tau phosphorylation
    Article Snippet: The supernatants were collected, and the concentrations were measured with the BCA assay (Thermo Fisher Scientific; Cat No. 23225). .. 10–20 μg protein were loaded onto 4–12% Bis-Tris polyacrylamide gel (Thermo Fisher Scientific; Cat No. NP0336BOX) Nitrocellulose (BioRad, Cat. No. 1620146) or PVDF membranes were used to transfer the protein in a BioRad Transblot for 11 minutes at 25 V, 25 A. Membranes were then blocked for 1 hour with Licor Intercept blocking buffer (Licor, Cat. No. 927-60001) at room temperature. .. Primary antibody was added in Licor Intercept block overnight at 4 °C.

    Semi Dry Blot:

    Article Title: The subset of M1-paired MHC class II conformers is critical for CD4+ T cell activation.
    Article Snippet: Major histocompatibility complex class II molecules present exogenous antigen-derived peptide to CD4+ T cells leading to T cell activation as well as signaling into the antigen-presenting cell.. Class II is an αβ heterodimer that forms 2 distinct conformers via differential pairing of transmembrane domain GxxxG motifs (i.e. M1and M2-paired class II).. Previous studies with mouse I-A and human HLA-DR class II used conformer-specific monoclonal antibodies (mAbs) to define unique immunobiological properties of each conformer.

    Membrane:

    Article Title: Alternative organelle targeting of OPA1 mediates fatty acid release from lipid droplets
    Article Snippet: Protein concentration of subcellular fractions and clear lysates were quantified using the Pierce BCA Protein Assay (Thermo Fisher Scientific). .. Equal amount of protein was denatured in 1x SDS-loading buffer at 95°C for 5–10 min and was loaded into an SDS-PAGE protein gel before being transferred to a nitrocellulose (Bio-Rad) or PVDF (Bio-Rad) membrane for western blotting. .. For western blotting, membranes were blocked with EveryBlot blocking buffer (Bio-Rad) for 1 h before being incubated with a primary antibody at room temperature for 1 h or at 4°C for overnight.

    Article Title: Fc gamma receptor binding modulates IgG clearance in cancer cachexia
    Article Snippet: Equivalent amounts of protein from each tissue lysate were determined via Bradford assay (Bio-Rad), then resolved by SDS-PAGE. .. Proteins were transferred onto nitrocellulose (Bio-Rad) membrane by semi-dry transfer. .. Membranes were then quickly washed with TBST (Tris-Buffer Saline + 0.1% Tween-20) and then incubated for 1 h with gentle rocking at room temperature in TBST + 5% bovine serum albumin (Alkali Scientific, Ft. Lauderdale, FL, USA) for blocking.

    Western Blot:

    Article Title: Alternative organelle targeting of OPA1 mediates fatty acid release from lipid droplets
    Article Snippet: Protein concentration of subcellular fractions and clear lysates were quantified using the Pierce BCA Protein Assay (Thermo Fisher Scientific). .. Equal amount of protein was denatured in 1x SDS-loading buffer at 95°C for 5–10 min and was loaded into an SDS-PAGE protein gel before being transferred to a nitrocellulose (Bio-Rad) or PVDF (Bio-Rad) membrane for western blotting. .. For western blotting, membranes were blocked with EveryBlot blocking buffer (Bio-Rad) for 1 h before being incubated with a primary antibody at room temperature for 1 h or at 4°C for overnight.

    Staining:

    Article Title: Identification of pathological CD133+ endothelial cells in venous malformations
    Article Snippet: Protein concentrations were determined with the Pierce Rapid Gold BCA Protein Assay Kit ( A53226 , Thermo Scientific) and measured using the ThermoFisher Multiskan Skyhigh Microplate Spectrophotometer. .. Equal amounts of protein were separated on a 10% SDS/PAGE gel, transferred to nitrocellulose (Bio-Rad, #162-0115), protein transfer visualized by ponceaus S staining, and blocked for 1 h at room temperature in 0.1M Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Tween-20 (1X TBST) containing 4% bovine serum albumin (BSA). ..



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