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human primary dermal fibroblasts normal neonatal hdfn  (ATCC)


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    Structured Review

    ATCC human primary dermal fibroblasts normal neonatal hdfn
    Human Primary Dermal Fibroblasts Normal Neonatal Hdfn, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 563 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neonatal/Primary+Dermal+Fibroblast+Normal%3B+Human%2C+Neonatal/pmc13253105-46-0-8
    Average 99 stars, based on 563 article reviews
    human primary dermal fibroblasts normal neonatal hdfn - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1
    Article Snippet: .. Primary human dermal microvascular endothelial cells (hMVEC-d) were purchased from Lonza (CC-2543), grown in Basal Medium (EBM-2) (CC-3156, Lonza) complete media (with supplements EGMTM-2 SingleQuotsTM Supplements [CC-4176, Lonza]) at 37°C under 5% CO 2 and used before passage five. hTERT-immortalized dermal microvascular endothelial cell, neonatal (CRL4060, ATCC), was used. hTERT was cultured in Vascular Cell Basal Medium (VCBM) (PCS-100-030, ATCC) with the microvascular endothelial cell growth kit-BBE (PCS-110-040, ATCC) +0.5 μg/mL puromycin (P8833, Sigma-Aldrich) according to the manufacturer’s instruction. .. When indicated, the following inhibitors were used: LY294002 (S1105, Selleckchem), U0123 (9903, Cell Signaling), cilengitide trifluoroacetate (S7077, Selleckchem), filipin III from Streptomyces filipinensis (48049-9 Sigma-Aldrich), dynasore (304448-55-3, EMD Millipore), desatinib (BMS-354825, S1021, Selleckchem), 2-Fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]-benzamide ([CK-666], SML0006, Sigma-Aldrich), imipramine hydrochloride (I0899, Sigma-Aldrich), SB203580, 5-(N-ethyl-N-isopropyl) amiloride (1154-25-2, Sigma-Aldrich), ML141 (7120335-5, EMD Millipore), EHop-016 (S7319, Selleckchem) and NSC 23766 trihydrochloride (S8031, Selleckchem).

    Article Title: Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1.
    Article Snippet: .. Primary human dermal microvascular endothelial cells (hMVEC-d) were purchased from Lonza (CC-2543), grown in Basal Medium (EBM-2) (CC-3156, Lonza) complete media (with supplements EGMTM-2 SingleQuotsTM Supplements [CC-4176, Lonza]) at 37°C under 5% CO2 and used before passage five. hTERT-immortalized dermal microvascular endothelial cell, neonatal (CRL4060, ATCC), was used. hTERT was cultured in Vascular Cell Basal Medium (VCBM) (PCS-100-030, ATCC) with the microvascular endothelial cell growth kit-BBE (PCS-110-040, ATCC) +0.5 μg/mL puromycin (P8833, Sigma-Aldrich) according to the manufacturer’s instruction. .. When indicated, the following inhibitors were used: LY294002 (S1105, Selleckchem), U0123 (9903, Cell Signaling), cilengitide trifluoroacetate (S7077, Selleckchem), filipin III from Streptomyces filipinensis (48049-9 Sigma-Aldrich), dynasore (304448-55-3, EMD Millipore), desatinib (BMS-354825, S1021, Selleckchem), 2-Fluoro-N-[2-(2-methyl-1Hindol-3-yl)ethyl]-benzamide ([CK-666], SML0006, Sigma-Aldrich), imipramine hydrochlor ide (I0899, Sigma-Aldrich), SB203580, 5-(N-ethyl-N-isopropyl) amiloride (1154-25-2, Sigma-Aldrich), ML141 (7120335-5, EMD Millipore), EHop-016 (S7319, Selleckchem) and NSC 23766 trihydrochloride (S8031, Selleckchem).

    other:

    Article Title: Transcriptional Elongation-Associated RNA Processing Errors in Induced Cellular Growth Arrest
    Article Snippet: Adult (PCS-201-012) and Neonatal (PCS-201-010) Human primary dermal fibroblasts were bought from ATCC.

    Article Title: Ex Vivo Biosafety and Efficacy Assessment of Advanced Chlorin e6 Nanoemulsions as a Drug Delivery System for Photodynamic Antitumoral Application
    Article Snippet: The Human, Neonatal (HDFn- ATCC ® PCS-201-010TM) cell line was established in 75 cm 2 culture flasks in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum.

    Modification:

    Article Title: Ex Vivo Biosafety and Efficacy Assessment of Advanced Chlorin e6 Nanoemulsions as a Drug Delivery System for Photodynamic Antitumoral Application.
    Article Snippet: The release kinetic was developed using the Sigma Plot 14 software, analyzing six conventional release models, Weibull, Korsmeyer–Peppas, Baker and Lonsdale, Higuchi, First Order, and Hixon and Crowell, obtaining data related to the specific parameter associated with each model. Molecules 2025, 30, 544 17 of 22 .. The Human, Neonatal (HDFn- ATCC® PCS-201-010TM) cell line was established in 75 cm2 culture flasks in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum. .. The cells were cultivated until confluence, followed by treatment with a 0.05% Gibco® trypsin solution (Waltham, MA, USA) to release them from the monolayer.



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