anti nedd8  (Proteintech)

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Promotes the Transport Clearance of Aβ Deposition by the BBB in APP/PS1 Mice. A Western blot analysis of Aβ and LRP1 expression in mouse hippocampa tissue along with quantitative assessment. B Immunofluorescence staining in mouse cortex using anti-CD31 (green) and anti-LRP1 (red) antibodies (Scale bar: 50 μm). C Immunohistochemical staining for Aβ in the hippocampal region of mice (Scale bar: 100 μm). Arrows indicate Aβ deposition. D Immunofluorescence staining in the hippocampal region of mice using anti-Aβ (red) and anti-IBA1 (green) antibodies (scale bars: 200 μm and 20 μm, respectively). E Immunofluorescence staining in the hippocampal region of mice using anti-Aβ (red) and anti-GFAP (green) antibodies (scale bars: 200 μm and 50 μm, respectively). Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control group: ** p < 0.01, *** p < 0.001 and relative to the AD group: # p < 0.05

Article Snippet: LRP1 Antibody , Wuhan Boster (BM4098) , 1:200.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Immunohistochemical staining, Control

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Improves Inflammation-induced TJs Damage in bEnd.3 Cells. A Schematic illustration of the Transwell BBB model and the optical parameters used for 808 nm laser irradiation. B FLU assay to evaluate the permeability of the monolayer bEnd.3 BBB model. C-E Immunofluorescence staining of bEnd.3 cells showing the expression of the TJs protein ZO-1 (red) and DAPI (blue), as well as LRP1 (red) with DAPI (blue), including quantitative analysis (Scale bar: 100 μm). F-I RT-PCR analysis of mRNA expression for TJs proteins Occludin, Claudin-5, ZO-1 and LRP1. J RT-PCR assessment of MMP-9 mRNA expression. K ELISA analysis of MMP-9 secretion in the supernatant of bEnd.3 cells. Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control group: * p < 0.05, ** p < 0.01, *** p < 0.001 and relative to the LPS group: # p < 0.05, ## p < 0.01, ### p < 0.001

Article Snippet: LRP1 Antibody , Wuhan Boster (BM4098) , 1:200.

Techniques: Irradiation, Permeability, Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Improves Inflammation-induced TJs Damage in bEnd.3 Cells by Activating AMPK. A Schematic diagram illustrating AMPK activation in bEnd.3 cells by PBM. B ELISA analysis of MMP-9 secretion in the supernatant of bEnd.3 cells following AICAR/Compound C treatment. C RT-PCR assessment of MMP-9 mRNA expression after treatment with AICAR/Compound C. D-E Immunofluorescence staining of the transport protein LRP1 (red) and DAPI (blue) in bEnd.3 cells following AICAR/Compound C treatment (Scale bar: 100 μm) with quantitative analysis. F–H RT-PCR analysis of Occludin, Claudin-5 and ZO-1 mRNA expression following AICAR/Compound C treatment. Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control + p-AMPK group: * p < 0.05, ** p < 0.01, *** p < 0.001 and relative to the Control + ko-AMPK group: # p < 0.05, ## p < 0.01, ### p < 0.001, relative to the p-AMPK group, ▲ p < 0.05, ▲▲ p < 0.01, ▲▲▲ p < 0.001

Article Snippet: LRP1 Antibody , Wuhan Boster (BM4098) , 1:200.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunofluorescence, Staining, Control

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Promotes the Transport Clearance of Aβ Deposition by the BBB in APP/PS1 Mice. A Western blot analysis of Aβ and LRP1 expression in mouse hippocampa tissue along with quantitative assessment. B Immunofluorescence staining in mouse cortex using anti-CD31 (green) and anti-LRP1 (red) antibodies (Scale bar: 50 μm). C Immunohistochemical staining for Aβ in the hippocampal region of mice (Scale bar: 100 μm). Arrows indicate Aβ deposition. D Immunofluorescence staining in the hippocampal region of mice using anti-Aβ (red) and anti-IBA1 (green) antibodies (scale bars: 200 μm and 20 μm, respectively). E Immunofluorescence staining in the hippocampal region of mice using anti-Aβ (red) and anti-GFAP (green) antibodies (scale bars: 200 μm and 50 μm, respectively). Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control group: ** p < 0.01, *** p < 0.001 and relative to the AD group: # p < 0.05

Article Snippet: LRP1 (85–92 kDa) , BOSTER (BM4098) , 1:2500.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Immunohistochemical staining, Control

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Improves Inflammation-induced TJs Damage in bEnd.3 Cells. A Schematic illustration of the Transwell BBB model and the optical parameters used for 808 nm laser irradiation. B FLU assay to evaluate the permeability of the monolayer bEnd.3 BBB model. C-E Immunofluorescence staining of bEnd.3 cells showing the expression of the TJs protein ZO-1 (red) and DAPI (blue), as well as LRP1 (red) with DAPI (blue), including quantitative analysis (Scale bar: 100 μm). F-I RT-PCR analysis of mRNA expression for TJs proteins Occludin, Claudin-5, ZO-1 and LRP1. J RT-PCR assessment of MMP-9 mRNA expression. K ELISA analysis of MMP-9 secretion in the supernatant of bEnd.3 cells. Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control group: * p < 0.05, ** p < 0.01, *** p < 0.001 and relative to the LPS group: # p < 0.05, ## p < 0.01, ### p < 0.001

Article Snippet: LRP1 (85–92 kDa) , BOSTER (BM4098) , 1:2500.

Techniques: Irradiation, Permeability, Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Improves Inflammation-induced TJs Damage in bEnd.3 Cells by Activating AMPK. A Schematic diagram illustrating AMPK activation in bEnd.3 cells by PBM. B ELISA analysis of MMP-9 secretion in the supernatant of bEnd.3 cells following AICAR/Compound C treatment. C RT-PCR assessment of MMP-9 mRNA expression after treatment with AICAR/Compound C. D-E Immunofluorescence staining of the transport protein LRP1 (red) and DAPI (blue) in bEnd.3 cells following AICAR/Compound C treatment (Scale bar: 100 μm) with quantitative analysis. F–H RT-PCR analysis of Occludin, Claudin-5 and ZO-1 mRNA expression following AICAR/Compound C treatment. Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control + p-AMPK group: * p < 0.05, ** p < 0.01, *** p < 0.001 and relative to the Control + ko-AMPK group: # p < 0.05, ## p < 0.01, ### p < 0.001, relative to the p-AMPK group, ▲ p < 0.05, ▲▲ p < 0.01, ▲▲▲ p < 0.001

Article Snippet: LRP1 (85–92 kDa) , BOSTER (BM4098) , 1:2500.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunofluorescence, Staining, Control