mucin2  (Bioss)

Journal: Chinese Medical Journal

Article Title: “One stone three birds”: Andrographis paniculata -derived exosome-like nanoparticles mitigate dextran sulfate sodium-induced colitis

doi: 10.1097/CM9.0000000000003651

Figure Lengend Snippet: APELNs protect the intestinal barrier in DSS-induced colitis. (A) AB/PAS (Alcian Blue/Periodic Acid-Schiff stain) staining of colon sections. (B) Quantification of goblet cells in colonic crypts. (C) APELNs reduced intestinal permeability. (D–G) qRT-PCR analysis of Claudin-1, ZO-1, MUC2, and OCLN expression in colon samples. (H, I) IF showing the localization of Claudin-1, ZO-1, MUC2, and OCLN. (J, K) WB analysis and relative protein expression of Claudin-1, ZO-1, MUC2, and OCLN in colon tissues. Data were shown as mean ± standard deviation. * P <0.05, † P <0.01, ‡ P <0.001, § P <0.0001. AB/PAS: Alcian blue/periodic acid-schiff stain; APELN: Andrographis paniculata -derived exosome-like nanoparticle; DAPI: 4′,6-diamidino-2-phenylindole; DSS: Dextran sodium sulfate; FITC-Dex: Fluorescein isothiocyanate-dextran permeability assay; IF: Immunofluorescence; MUC2: Mucin2; ns: Not significant; OCLN: Occludin; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; WB: Western blotting; ZO-1: Zonula occludens-1.

Article Snippet: Samples were incubated overnight at 4°C with primary antibodies, including anti-Mucin2 (MUC2) (GB11344, Servicebio, China), anti-occludin (OCLN) ( GB111401 , ab216327, Servicebio), anti-ZO-1 ( GB111402 , Servicebio), and anti-Claudin-1 (GB15032, Servicebio).

Techniques: Staining, Permeability, Quantitative RT-PCR, Expressing, Standard Deviation, Derivative Assay, FITC-Dextran Permeability Assay, Immunofluorescence, Reverse Transcription, Polymerase Chain Reaction, Western Blot

Journal: Chinese Medical Journal

Article Title: “One stone three birds”: Andrographis paniculata -derived exosome-like nanoparticles mitigate dextran sulfate sodium-induced colitis

doi: 10.1097/CM9.0000000000003651

Figure Lengend Snippet: APELNs enhance TJ protein expression in IECs. (A, B) qRT-PCR analysis of Claudin-1, ZO-1, MUC2, and OCLN in NCM460 and HCT116 cells. (C, D, E) WB analysis and relative protein expression of Claudin-1, ZO-1, MUC2, and OCLN in NCM460 and HCT116 cells. (F, G) IF images showing the expression of Claudin-1, ZO-1, MUC2, and OCLN. Bar: 20 μm. * P <0.05, † P <0.01, ‡ P <0.001, § P <0.0001. APELN: Andrographis paniculata -derived exosome-like nanoparticle; DAPI: 4′,6-diamidino-2-phenylindole; IEC: Intestinal epithelial cell; LPS: Lipopolysaccharide; MUC2: Mucin2; OCLN: Occludin; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; TJ: Tight junction; WB: Western blotting; ZO-1: Zonula occludens-1.

Article Snippet: Samples were incubated overnight at 4°C with primary antibodies, including anti-Mucin2 (MUC2) (GB11344, Servicebio, China), anti-occludin (OCLN) ( GB111401 , ab216327, Servicebio), anti-ZO-1 ( GB111402 , Servicebio), and anti-Claudin-1 (GB15032, Servicebio).

Techniques: Expressing, Quantitative RT-PCR, Derivative Assay, Reverse Transcription, Polymerase Chain Reaction, Western Blot