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    Structured Review

    MedChemExpress medium
    Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtco2/MTCO2+Antibody/10__1038_slash_s41522___026___01159___5-397-18-14
    Average 97 stars, based on 36 article reviews
    medium - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Activation of ERBB4 Pathway Inhibits Pathological Transdifferentiation of Lung Epithelial Progenitors into CD66c + Basal Cells in Severe Lung Injury.
    Article Snippet: .. Medium was refreshed every 2–3 days, and organoids ere cultured at 37◦C in a humidified incubator with 5% CO2 . or functional perturbation experiments, organoids were treated ith CHIR99021 (3 μM; MedChemExpress (MCE), USA), recominant human NRG1 (50 ng/mL; Sino Biological, China), or he ERBB4 inhibitor AG1478 (5 μM; T2047, TargetMol, USA). or pathway modulation, BMP signaling was inhibited by withrawing Noggin (Nog; Sino Biological, China) and adding the MP type I receptor inhibitor LDN193189 (LDN; 100 nM; T1935, argetMol, USA), while the MEK inhibitor U0126 (2.5 μM; T21332, argetMol, USA) was applied to block MAPK signaling. ..

    Article Title: Nanocellulose Alleviates Intrahepatic Cholestasis of Pregnancy via Gut Microbiota-Mediated Bile Acid Homeostasis.
    Article Snippet: .. The human colon epithelial cell 2 of 25 line Caco-2 was cultured in DMEM supplemented with 20% fetal bovine serum (FBS) at 37◦C in a humidified atmosphere containing 5% CO2 , and treated with CDCA (100 μm ) or the selective FXR agonist GW4064 (1 μm ; Cat. No. HY-50108, MCE) for 24 h. Total RNA and protein were extracted for qPCR and Western blot analyses of FXR signaling-related genes and proteins. ..

    Functional Assay:

    Article Title: Activation of ERBB4 Pathway Inhibits Pathological Transdifferentiation of Lung Epithelial Progenitors into CD66c + Basal Cells in Severe Lung Injury.
    Article Snippet: .. Medium was refreshed every 2–3 days, and organoids ere cultured at 37◦C in a humidified incubator with 5% CO2 . or functional perturbation experiments, organoids were treated ith CHIR99021 (3 μM; MedChemExpress (MCE), USA), recominant human NRG1 (50 ng/mL; Sino Biological, China), or he ERBB4 inhibitor AG1478 (5 μM; T2047, TargetMol, USA). or pathway modulation, BMP signaling was inhibited by withrawing Noggin (Nog; Sino Biological, China) and adding the MP type I receptor inhibitor LDN193189 (LDN; 100 nM; T1935, argetMol, USA), while the MEK inhibitor U0126 (2.5 μM; T21332, argetMol, USA) was applied to block MAPK signaling. ..

    Blocking Assay:

    Article Title: Activation of ERBB4 Pathway Inhibits Pathological Transdifferentiation of Lung Epithelial Progenitors into CD66c + Basal Cells in Severe Lung Injury.
    Article Snippet: .. Medium was refreshed every 2–3 days, and organoids ere cultured at 37◦C in a humidified incubator with 5% CO2 . or functional perturbation experiments, organoids were treated ith CHIR99021 (3 μM; MedChemExpress (MCE), USA), recominant human NRG1 (50 ng/mL; Sino Biological, China), or he ERBB4 inhibitor AG1478 (5 μM; T2047, TargetMol, USA). or pathway modulation, BMP signaling was inhibited by withrawing Noggin (Nog; Sino Biological, China) and adding the MP type I receptor inhibitor LDN193189 (LDN; 100 nM; T1935, argetMol, USA), while the MEK inhibitor U0126 (2.5 μM; T21332, argetMol, USA) was applied to block MAPK signaling. ..

    Incubation:

    Article Title: Mitochondrial activity promotes neutrophil degranulation and endothelial dysfunction in systemic infections.
    Article Snippet: .. Cells were incubated at 37 °C, 5% CO2 for 15 min to allow attachment after which vehicle (DMSO) or SkQ1 (MedChemExpress) was added to a final concentration of 500 nM. ..

    Article Title: Ferroptosis inhibition and mitochondrial rescue: a novel mechanism of emodin in rheumatoid arthritis.
    Article Snippet: The nuclei were stained with Hoechst, and fluorescence images were quickly obtained with a DMi8 microscope (Leica, Germany). .. RAW264.7 cells were placed in 96-well plates and incubated at 37°C in an atmosphere of 5% CO2 for 24 h. When the cells achieved approximately 70% confluence, they were exposed to LPS (100ng/mL) along with varying concentrations of EMO (0, 6.25, 12.5, 25, 50, 100μM) for a duration of 24 h. Following this treatment, CCK-8 reagent (HY-K0301, MedChemExpress, USA) was introduced according to the manufacturer's guidance and allowed to incubate. ..

    Concentration Assay:

    Article Title: Mitochondrial activity promotes neutrophil degranulation and endothelial dysfunction in systemic infections.
    Article Snippet: .. Cells were incubated at 37 °C, 5% CO2 for 15 min to allow attachment after which vehicle (DMSO) or SkQ1 (MedChemExpress) was added to a final concentration of 500 nM. ..

    Control:

    Article Title: Metabolites released from apoptotic cells in central nervous system orchestrates the pathological process of Alzheimer disease through improving autophagy.
    Article Snippet: Apoptosis, a programmed cell death process activated in Alzheimer disease (AD), is not limited to neurons but extends to all cell types within the central nervous system (CNS).. However, how apoptotic cells mediate their impact on surrounding cells and contribute to the pathological progression of AD remains largely unclear.. Here, we report that in 5×FAD mice, cells surrounding amyloid-β (Aβ) plaques undergo apoptosis, which occurs concurrently with elevated macroautophagy/autophagy.

    Staining:

    Article Title: Biofilm metabolic heterogeneity drives macrophage stress and immune evasion
    Article Snippet: CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) was prepared as a 20 nM working solution in completemedium and incubated for 1 h at 37 °C in 5%CO2 protected from light, after which cells were imaged using the GFP channel on the EVOS M7000 or analyzed using the corresponding preset parameters on the Bigfoot. .. For lysosomal dual staining, LysoTracker Red DND-99 (50 nM, ThermoFisher Scientific) and LysoSensorDND-189 (1 μM,MCE)were codissolved in serum-free medium to prepare working solutions and coincubated for 30min at 37 °C in 5% CO2; cells were subsequently imaged using the Texas Red and GFP channels on the EVOS M7000, or analyzed using the corresponding preset parameters on the Bigfoot. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Nanocellulose Alleviates Intrahepatic Cholestasis of Pregnancy via Gut Microbiota-Mediated Bile Acid Homeostasis.
    Article Snippet: .. The human colon epithelial cell 2 of 25 line Caco-2 was cultured in DMEM supplemented with 20% fetal bovine serum (FBS) at 37◦C in a humidified atmosphere containing 5% CO2 , and treated with CDCA (100 μm ) or the selective FXR agonist GW4064 (1 μm ; Cat. No. HY-50108, MCE) for 24 h. Total RNA and protein were extracted for qPCR and Western blot analyses of FXR signaling-related genes and proteins. ..

    Western Blot:

    Article Title: Nanocellulose Alleviates Intrahepatic Cholestasis of Pregnancy via Gut Microbiota-Mediated Bile Acid Homeostasis.
    Article Snippet: .. The human colon epithelial cell 2 of 25 line Caco-2 was cultured in DMEM supplemented with 20% fetal bovine serum (FBS) at 37◦C in a humidified atmosphere containing 5% CO2 , and treated with CDCA (100 μm ) or the selective FXR agonist GW4064 (1 μm ; Cat. No. HY-50108, MCE) for 24 h. Total RNA and protein were extracted for qPCR and Western blot analyses of FXR signaling-related genes and proteins. ..

    96 Well Plates:

    Article Title: Ferroptosis inhibition and mitochondrial rescue: a novel mechanism of emodin in rheumatoid arthritis.
    Article Snippet: The nuclei were stained with Hoechst, and fluorescence images were quickly obtained with a DMi8 microscope (Leica, Germany). .. RAW264.7 cells were placed in 96-well plates and incubated at 37°C in an atmosphere of 5% CO2 for 24 h. When the cells achieved approximately 70% confluence, they were exposed to LPS (100ng/mL) along with varying concentrations of EMO (0, 6.25, 12.5, 25, 50, 100μM) for a duration of 24 h. Following this treatment, CCK-8 reagent (HY-K0301, MedChemExpress, USA) was introduced according to the manufacturer's guidance and allowed to incubate. ..

    CCK-8 Assay:

    Article Title: Ferroptosis inhibition and mitochondrial rescue: a novel mechanism of emodin in rheumatoid arthritis.
    Article Snippet: The nuclei were stained with Hoechst, and fluorescence images were quickly obtained with a DMi8 microscope (Leica, Germany). .. RAW264.7 cells were placed in 96-well plates and incubated at 37°C in an atmosphere of 5% CO2 for 24 h. When the cells achieved approximately 70% confluence, they were exposed to LPS (100ng/mL) along with varying concentrations of EMO (0, 6.25, 12.5, 25, 50, 100μM) for a duration of 24 h. Following this treatment, CCK-8 reagent (HY-K0301, MedChemExpress, USA) was introduced according to the manufacturer's guidance and allowed to incubate. ..



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    A ATP levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). B ATP levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). C Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, <t>MTCO2</t> and COX5A levels in Lenti-BACH1 and Lenti-Control cells by RT-qPCR ( n = 3 × 3). D Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, MTCO2 and COX5A levels in HepG2 BACH1-KO and HepG2 cells by RT-qPCR ( n = 3 × 3). E Western blotting analysis and quantification of NDUFS1, NDUFC2, SDHB, MTCO1 and MTCO2 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3). F Western blotting analysis and quantification of NDUFS1, NDUFC2, MTCO1 and MTCO2 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3). G, H The contents of pyruvate G and lactate H in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). I, J The contents of pyruvate I and lactate J in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). K RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA, GPI, PGAM1, PGK1, PKM, ALDOA and PDK1 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). L RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). M RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). N Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL, GAPDH and HK1 levels in Lenti-BACH1 and Lenti-Control cells by Western blot ( n = 3). O Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1 and GAPDH levels in HepG2 BACH1-KO and HepG2 cells by Western blot ( n = 3). P Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL and GAPDH levels in HCCLM3 BACH1-KO and HCCLM3 cells by Western blot ( n = 3). Data are presented with mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001).
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    A ATP levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). B ATP levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). C Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, <t>MTCO2</t> and COX5A levels in Lenti-BACH1 and Lenti-Control cells by RT-qPCR ( n = 3 × 3). D Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, MTCO2 and COX5A levels in HepG2 BACH1-KO and HepG2 cells by RT-qPCR ( n = 3 × 3). E Western blotting analysis and quantification of NDUFS1, NDUFC2, SDHB, MTCO1 and MTCO2 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3). F Western blotting analysis and quantification of NDUFS1, NDUFC2, MTCO1 and MTCO2 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3). G, H The contents of pyruvate G and lactate H in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). I, J The contents of pyruvate I and lactate J in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). K RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA, GPI, PGAM1, PGK1, PKM, ALDOA and PDK1 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). L RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). M RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). N Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL, GAPDH and HK1 levels in Lenti-BACH1 and Lenti-Control cells by Western blot ( n = 3). O Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1 and GAPDH levels in HepG2 BACH1-KO and HepG2 cells by Western blot ( n = 3). P Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL and GAPDH levels in HCCLM3 BACH1-KO and HCCLM3 cells by Western blot ( n = 3). Data are presented with mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001).
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    97
    MedChemExpress culture conditions
    A ATP levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). B ATP levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). C Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, <t>MTCO2</t> and COX5A levels in Lenti-BACH1 and Lenti-Control cells by RT-qPCR ( n = 3 × 3). D Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, MTCO2 and COX5A levels in HepG2 BACH1-KO and HepG2 cells by RT-qPCR ( n = 3 × 3). E Western blotting analysis and quantification of NDUFS1, NDUFC2, SDHB, MTCO1 and MTCO2 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3). F Western blotting analysis and quantification of NDUFS1, NDUFC2, MTCO1 and MTCO2 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3). G, H The contents of pyruvate G and lactate H in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). I, J The contents of pyruvate I and lactate J in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). K RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA, GPI, PGAM1, PGK1, PKM, ALDOA and PDK1 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). L RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). M RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). N Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL, GAPDH and HK1 levels in Lenti-BACH1 and Lenti-Control cells by Western blot ( n = 3). O Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1 and GAPDH levels in HepG2 BACH1-KO and HepG2 cells by Western blot ( n = 3). P Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL and GAPDH levels in HCCLM3 BACH1-KO and HCCLM3 cells by Western blot ( n = 3). Data are presented with mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001).
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    (a) Western blot for COX2, COX1 and COX4 in BCS-treated SEM (day 8), NALM6 (day 6) and MOLT16 (day 8) cells. (b) Blue-native (BN-PAGE) followed by western blot detection of indicated subunits in native complex I-III in BCS treated SEM cells (day 8) or NALM6 cells (day 6). Representative data of two biological replicates shown. Additional replicate shown in Source Data. (c-d) Levels of NAD, NADH and the NAD/NADH ratio were determined by LC-MS targeted metabolite profiling in (c) SLC31A1-KO SEM cells or (d) BCS treated SEM (day 8), NALM6 (day 6) and MOLT16 (day 8) cells. (e) Levels of succinate, fumarate, and succinate/fumarate ratio in BCS treated SEM and NALM6 cells as determined by targeted LC-MS metabolite profiling. (f-g) Seahorse assay to measure OCR in permeabilized BCS-treated (f) SEM (day 8) or (g) NALM6 (day 6) cells following treatment with complex I or II substrates. (h-i) MitoStress Seahorse assay performed on SLC31A1-KO SEM cells with (h) eGFP or SLC31A1 rescue, or (i) 100 μM CuCl 2 pre-treatment for 24 hours. For Seahorse assays, plotted data are mean ± SD with 4–6 technical replicates (wells). Other plotted data are mean±SD, p-values from Šídák's multiple comparisons test. Each dot represents a biological replicate. BCS and CuCl 2 were used at 50 μM. OE = overexpression, BCS = bathocuproinedisulfonic acid, oligo = oligomycin, AA = antimycin A, rot = rotenone, FCCP = carbonyl cyanide-p-trifluoromethoxyphenylhydrazone, perm = XF Plasma Membrane Permeabilizer, pyr/mal = pyruvate and malate, succ = succinate.

    Journal: Nature cancer

    Article Title: Copper depletion inhibits electron transport chain activity and nucleotide synthesis to boost leukemia therapy

    doi: 10.1038/s43018-026-01177-4

    Figure Lengend Snippet: (a) Western blot for COX2, COX1 and COX4 in BCS-treated SEM (day 8), NALM6 (day 6) and MOLT16 (day 8) cells. (b) Blue-native (BN-PAGE) followed by western blot detection of indicated subunits in native complex I-III in BCS treated SEM cells (day 8) or NALM6 cells (day 6). Representative data of two biological replicates shown. Additional replicate shown in Source Data. (c-d) Levels of NAD, NADH and the NAD/NADH ratio were determined by LC-MS targeted metabolite profiling in (c) SLC31A1-KO SEM cells or (d) BCS treated SEM (day 8), NALM6 (day 6) and MOLT16 (day 8) cells. (e) Levels of succinate, fumarate, and succinate/fumarate ratio in BCS treated SEM and NALM6 cells as determined by targeted LC-MS metabolite profiling. (f-g) Seahorse assay to measure OCR in permeabilized BCS-treated (f) SEM (day 8) or (g) NALM6 (day 6) cells following treatment with complex I or II substrates. (h-i) MitoStress Seahorse assay performed on SLC31A1-KO SEM cells with (h) eGFP or SLC31A1 rescue, or (i) 100 μM CuCl 2 pre-treatment for 24 hours. For Seahorse assays, plotted data are mean ± SD with 4–6 technical replicates (wells). Other plotted data are mean±SD, p-values from Šídák's multiple comparisons test. Each dot represents a biological replicate. BCS and CuCl 2 were used at 50 μM. OE = overexpression, BCS = bathocuproinedisulfonic acid, oligo = oligomycin, AA = antimycin A, rot = rotenone, FCCP = carbonyl cyanide-p-trifluoromethoxyphenylhydrazone, perm = XF Plasma Membrane Permeabilizer, pyr/mal = pyruvate and malate, succ = succinate.

    Article Snippet: Immunoblotting was performed using the following primary antibodies: For reducing and denaturing western blot: vinculin (CST 13901; 1:2500) Beta actin (CST 8457; 1:2000) GAPDH (CST 2118L; 1:2000) MT-CO1/COX1 (abcam ab14705; 1:2000) COX4I1/COX4 (CST 4850; 1:1000) COX2 (Proteintech 55070–1-AP; 1:5000) DHODH (Santa Cruz E-8 sc-166348; 1:500) SDHA (CST 5839S; 1:1000) SLC31A1 (CST 13086S; 1:500) Beta tubulin (CST 2128P; 1:1000) Human Total OxPhos Cocktail (abcam ab110411; 1:1000) VDAC1/porin (abcam ab154856; 1:1000) Total ERK1/2 (CST 4696S; 1:1000) Phospho-ERK1/2 (CST 4370L; 1:1000) Primary antibodies were diluted in Signal Enhancer HIKARI Solution 1 (Nacalai USA NU00102).

    Techniques: Activity Assay, Western Blot, Liquid Chromatography with Mass Spectroscopy, Profiling, Over Expression, Clinical Proteomics, Membrane

    A ATP levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). B ATP levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). C Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, MTCO2 and COX5A levels in Lenti-BACH1 and Lenti-Control cells by RT-qPCR ( n = 3 × 3). D Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, MTCO2 and COX5A levels in HepG2 BACH1-KO and HepG2 cells by RT-qPCR ( n = 3 × 3). E Western blotting analysis and quantification of NDUFS1, NDUFC2, SDHB, MTCO1 and MTCO2 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3). F Western blotting analysis and quantification of NDUFS1, NDUFC2, MTCO1 and MTCO2 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3). G, H The contents of pyruvate G and lactate H in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). I, J The contents of pyruvate I and lactate J in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). K RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA, GPI, PGAM1, PGK1, PKM, ALDOA and PDK1 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). L RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). M RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). N Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL, GAPDH and HK1 levels in Lenti-BACH1 and Lenti-Control cells by Western blot ( n = 3). O Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1 and GAPDH levels in HepG2 BACH1-KO and HepG2 cells by Western blot ( n = 3). P Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL and GAPDH levels in HCCLM3 BACH1-KO and HCCLM3 cells by Western blot ( n = 3). Data are presented with mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Journal: Cell Death & Disease

    Article Title: BACH1 stabilization by antioxidants facilitates hepatocellular carcinoma metastasis

    doi: 10.1038/s41419-026-08865-0

    Figure Lengend Snippet: A ATP levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). B ATP levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). C Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, MTCO2 and COX5A levels in Lenti-BACH1 and Lenti-Control cells by RT-qPCR ( n = 3 × 3). D Detection of NDUFS1, NDUFC2, NDUFV2, SDHA, SDHB, SDHC, MTCYB, UQCRFS1, MTCO1, MTCO2 and COX5A levels in HepG2 BACH1-KO and HepG2 cells by RT-qPCR ( n = 3 × 3). E Western blotting analysis and quantification of NDUFS1, NDUFC2, SDHB, MTCO1 and MTCO2 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3). F Western blotting analysis and quantification of NDUFS1, NDUFC2, MTCO1 and MTCO2 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3). G, H The contents of pyruvate G and lactate H in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). I, J The contents of pyruvate I and lactate J in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). K RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA, GPI, PGAM1, PGK1, PKM, ALDOA and PDK1 levels in Lenti-BACH1 and Lenti-Control cells ( n = 3 × 3). L RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HepG2 BACH1-KO and HepG2 cells ( n = 3 × 3). M RT-qPCR analysis of HK1, HK2, GLUT1, GLUT4, PFKL, GAPDH, LDHA and PDK1 levels in HCCLM3 BACH1-KO and HCCLM3 cells ( n = 3 × 3). N Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL, GAPDH and HK1 levels in Lenti-BACH1 and Lenti-Control cells by Western blot ( n = 3). O Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1 and GAPDH levels in HepG2 BACH1-KO and HepG2 cells by Western blot ( n = 3). P Detection and quantification of HK2, GLUT1, GLUT3, GLUT4, LDHA, PDK1, PFKL and GAPDH levels in HCCLM3 BACH1-KO and HCCLM3 cells by Western blot ( n = 3). Data are presented with mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Article Snippet: Primary antibodies used in the immunoblotting process including BACH1 (sc-271211, Santa Cruz Biotechnology), NRF2 (ab62352), GSR (ab124995), GCLC (ab207777), NQO1 (ab80588), GCLM (ab126704), HO-1 (ab52947), GPX1 (ab108427), XBP1 (ab109221), IRE1 (ab37073), eIF2A (ab169528), PERK (ab229912), SNAL1 (ab216347), SNAIL2 (ab302780), NDUFC2 (ab192265) (all these antibodies purchased from Abcam), HK1 (D221854), PFKL (D222865), SDHB (D262175) (these antibodies purchased from Sangon Biotechnology, China), SOD1 (AF3418, R&D Systems, USA), G6PD (A1537), TXN2 (A12591), p-PERK (AP1501), GLUT4 (A7637), LDHA(A1146), GLUT1 (A11208), MTCO1 (A17889), MTCO2 (A11913) (all eight antibodies purchased from ABclonal, Wuhan, China), FGF21 (A5710, bimake, USA), HSP60 (A5629, bimake, USA), GRP75 (A5420, bimake, USA), CDH1(20874-1-AP, Proteintech Group), CDH2 (22018-1-AP, Proteintech Group), Vimentin (60330-1-Ig, Proteintech Group), GAPDH (#2118, Cell Signaling Technology), HK2 (#2867, Cell Signaling Technology), β-actin (TA-09, from ZSGB-BIO, Beijing, China) and GLUT3 (bs-22374R, BIOSS, Beijing, China).

    Techniques: Control, Quantitative RT-PCR, Western Blot