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mspi  (New England Biolabs)
97
New England Biolabs mspi
Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mspi/pmc13171454-89-8-10?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
mspi - by Bioz Stars, 2026-07
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mspi restriction enzymes  (New England Biolabs)
97
New England Biolabs mspi restriction enzymes
Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
Mspi Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mspi/10__3390_slash_plants15101505-218-6-9?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
mspi restriction enzymes - by Bioz Stars, 2026-07
97/100 stars
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mspi restriction endonucleases  (New England Biolabs)
97
New England Biolabs mspi restriction endonucleases
Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
Mspi Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mspi/pm42074589-79-14-17?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
mspi restriction endonucleases - by Bioz Stars, 2026-07
97/100 stars
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mspi enzymes  (New England Biolabs)
97
New England Biolabs mspi enzymes
Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
Mspi Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mspi/bio_rxiv__64898__2026__04__28__721500-32-13-15?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
mspi enzymes - by Bioz Stars, 2026-07
97/100 stars
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pbr322 dna mspi digest  (New England Biolabs)
95
New England Biolabs pbr322 dna mspi digest
Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
Pbr322 Dna Mspi Digest, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mspi/pm41997936-489-12-15?v=New+England+Biolabs
Average 95 stars, based on 1 article reviews
pbr322 dna mspi digest - by Bioz Stars, 2026-07
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restriction enzymes mspi  (New England Biolabs)
97
New England Biolabs restriction enzymes mspi
Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
Restriction Enzymes Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mspi/pmc13121113-125-6-11?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
restriction enzymes mspi - by Bioz Stars, 2026-07
97/100 stars
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quick load pbr322 dna mspi molecular marker  (New England Biolabs)
96
New England Biolabs quick load pbr322 dna mspi molecular marker
Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
Quick Load Pbr322 Dna Mspi Molecular Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mspi/pmc13069499-105-62-68?v=New+England+Biolabs
Average 96 stars, based on 1 article reviews
quick load pbr322 dna mspi molecular marker - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier

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Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, input DNA fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease MspI then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.

Journal: Nucleic Acids Research

Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing

doi: 10.1093/nar/gkag385

Figure Lengend Snippet: Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, input DNA fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease MspI then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.

Article Snippet: Sheared or unsheared genomic DNA was digested with MspI (R0106M, NEB), end-repaired, 3′-dA-tailed, and ligated to adapters using the NEBNext Ultra II modules (E7546L and E7595L, NEB).

Techniques: Sequencing, Preserving, Methylation, Amplification, Ligation, Clinical Proteomics, Formalin-fixed Paraffin-Embedded