mspi  (Promega)

 
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    Name:
    MspI
    Description:
    Restriction enzyme that cuts DNA at 37°C and leaves a 5 overhang
    Catalog Number:
    r6401
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Cloning DNA Markers Restriction Enzymes
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    Structured Review

    Promega mspi
    UPGMA cluster analysis based on Jaccard similarity coefficients of all samples with a combination of all three enzymes (AciI, <t>MspI,</t> and <t>HaeIII).</t>
    Restriction enzyme that cuts DNA at 37°C and leaves a 5 overhang
    https://www.bioz.com/result/mspi/product/Promega
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    mspi - by Bioz Stars, 2020-07
    94/100 stars

    Images

    1) Product Images from "Comparison of Bacteroides-Prevotella 16S rRNA Genetic Markers for Fecal Samples from Different Animal Species"

    Article Title: Comparison of Bacteroides-Prevotella 16S rRNA Genetic Markers for Fecal Samples from Different Animal Species

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.71.10.5999-6007.2005

    UPGMA cluster analysis based on Jaccard similarity coefficients of all samples with a combination of all three enzymes (AciI, MspI, and HaeIII).
    Figure Legend Snippet: UPGMA cluster analysis based on Jaccard similarity coefficients of all samples with a combination of all three enzymes (AciI, MspI, and HaeIII).

    Techniques Used:

    Examples of T-RFLP peaks resulting from cow and human fecal samples cut with AciI, MspI, and HaeIII.
    Figure Legend Snippet: Examples of T-RFLP peaks resulting from cow and human fecal samples cut with AciI, MspI, and HaeIII.

    Techniques Used:

    Related Articles

    Amplification:

    Article Title: Comparison of Bacteroides-Prevotella 16S rRNA Genetic Markers for Fecal Samples from Different Animal Species
    Article Snippet: .. Three different restriction digests were performed on each sample by adding 7 μl of amplified product to 5 U of the following restriction enzymes in separate reactions: AciI (New England BioLabs, Beverly MA), HaeIII (Promega, Madison, WI), and MspI (Promega). ..

    Article Title: Association between Serotonin-Related Polymorphisms in 5HT2A, TPH1, TPH2 Genes and Bipolar Disorder in Korean Population
    Article Snippet: .. The amplified DNA was digested with the restriction enzyme MspI (Promega, USA), which cuts at the 1438G sites. .. The product was electrophoresed on 3% agarose gels and stained with ethidium bromide.

    Terminal Restriction Fragment Length Polymorphism:

    Article Title: Ruminal Prevotella spp. May Play an Important Role in the Conversion of Plant Lignans into Human Health Beneficial Antioxidants
    Article Snippet: .. PCR-T-RFLP analysis of the total bacterial population PCR T-RFLP was conducted as described by Huws et al. , in triplicate with subsequent pooling and use of the restriction enzymes Hae III or Msp I (Promega, Madison, USA). .. Restriction digested were run on a ABI3130xl DNA sequencer (Applied Biosystems, CA, USA) and T-RFs checked and exported using Genemapper software (Applied Biosystems, CA, USA).

    Purification:

    Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
    Article Snippet: .. The restriction reactions were performed for 16 h at 37 °C using a digestion mixture containing 6 µl of the purified PCR products, 1 X reaction buffer (Promega), 0.1 µg µl−1 BSA and 20 U Cfo I or Msp I (Promega) in a final volume of 20 µl. .. Each digested sample (3 µl) was mixed with 0.3 µl GeneScan 600-LIZ® (Applied Biosystems) size standard and 10 µl Hi-Di® Formamide (Applied Biosystems) in a 96-well plate and denatured at 90 °C for 3 min.

    Dot Blot:

    Article Title: Methylation profile of a satellite DNA constituting the intercalary G+C-rich heterochromatin of the cut trough shell Spisula subtruncata (Bivalvia, Mactridae)
    Article Snippet: .. Southern and dot blot hybridisation Genomic DNAs were digested with Hae III (Fermentas), Hpa II (Fermentas), Msp I (Promega), Pvu II (New England Biolabs) and Taq I (Roche). .. Restriction products were resolved by gel electrophoresis, transferred to positively charged nylon membranes (Roche), and hybridized overnight with the DIG-labelled SSUsat probe.

    Polymerase Chain Reaction:

    Article Title: Ruminal Prevotella spp. May Play an Important Role in the Conversion of Plant Lignans into Human Health Beneficial Antioxidants
    Article Snippet: .. PCR-T-RFLP analysis of the total bacterial population PCR T-RFLP was conducted as described by Huws et al. , in triplicate with subsequent pooling and use of the restriction enzymes Hae III or Msp I (Promega, Madison, USA). .. Restriction digested were run on a ABI3130xl DNA sequencer (Applied Biosystems, CA, USA) and T-RFs checked and exported using Genemapper software (Applied Biosystems, CA, USA).

    Article Title: Absence of Apo B R3500Q Mutation among Kelantanese Malays with Hyperlipidaemia
    Article Snippet: .. The PCR products were then digested with Msp I (Promega, U.S.A.) at 37ºC for 3 hours. .. The reaction mixture was prepared by mixing 10 μl PCR product, 10 U restriction enzyme and 2 μl restriction enzyme buffer in a 20 ll volume.

    Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
    Article Snippet: .. The restriction reactions were performed for 16 h at 37 °C using a digestion mixture containing 6 µl of the purified PCR products, 1 X reaction buffer (Promega), 0.1 µg µl−1 BSA and 20 U Cfo I or Msp I (Promega) in a final volume of 20 µl. .. Each digested sample (3 µl) was mixed with 0.3 µl GeneScan 600-LIZ® (Applied Biosystems) size standard and 10 µl Hi-Di® Formamide (Applied Biosystems) in a 96-well plate and denatured at 90 °C for 3 min.

    Hybridization:

    Article Title: Methylation profile of a satellite DNA constituting the intercalary G+C-rich heterochromatin of the cut trough shell Spisula subtruncata (Bivalvia, Mactridae)
    Article Snippet: .. Southern and dot blot hybridisation Genomic DNAs were digested with Hae III (Fermentas), Hpa II (Fermentas), Msp I (Promega), Pvu II (New England Biolabs) and Taq I (Roche). .. Restriction products were resolved by gel electrophoresis, transferred to positively charged nylon membranes (Roche), and hybridized overnight with the DIG-labelled SSUsat probe.

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  • 94
    Promega msp i
    MDS ordination plot of P. clavata bacterial communities. 2-D scatter plots of T-RFLP profiles from P. clavata colonies sampled at 3 sites in winter and summer from 2007 to 2010 are based on the Dice similarity matrix for the T-RFLP data retrieved from the amplified bacterial 16S rDNA digested with <t>Cfo</t> I (A) and <t>Msp</t> I (B). Each symbol represents the bacterial community of an individual sample from Riou (square), Medes (triangle) or Scandola (circle), and the colors correspond to different sampling seasons. Sample clusters are based on coordinates determined with the k-means method. Dotted ellipses contain 50% (grey dots) or 95% (dark dots) of the points that contribute to the cluster. The centers of the confidence ellipses are identified with a cross.
    Msp I, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msp i/product/Promega
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    msp i - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    88
    Promega ecori mspi
    Methylation profiles of pig tissue DNA with the primer combinations H-M+TAA/E+AGT. (A) The profile from F-MSAP; (B) Data from (A) quantitated using GeneScan Analysis software; H and M refer to digestion with <t>EcoRI/HpaII</t> and <t>EcoRI/MspI.</t> Lanes 1–7 represent seven tissues, namely muscle, heart, liver, spleen, lung, kidney and stomach; M: GeneScan™ -500ROX™ size standard (represent 200 bp and 220 bp).
    Ecori Mspi, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori mspi/product/Promega
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecori mspi - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    MDS ordination plot of P. clavata bacterial communities. 2-D scatter plots of T-RFLP profiles from P. clavata colonies sampled at 3 sites in winter and summer from 2007 to 2010 are based on the Dice similarity matrix for the T-RFLP data retrieved from the amplified bacterial 16S rDNA digested with Cfo I (A) and Msp I (B). Each symbol represents the bacterial community of an individual sample from Riou (square), Medes (triangle) or Scandola (circle), and the colors correspond to different sampling seasons. Sample clusters are based on coordinates determined with the k-means method. Dotted ellipses contain 50% (grey dots) or 95% (dark dots) of the points that contribute to the cluster. The centers of the confidence ellipses are identified with a cross.

    Journal: PLoS ONE

    Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea

    doi: 10.1371/journal.pone.0057385

    Figure Lengend Snippet: MDS ordination plot of P. clavata bacterial communities. 2-D scatter plots of T-RFLP profiles from P. clavata colonies sampled at 3 sites in winter and summer from 2007 to 2010 are based on the Dice similarity matrix for the T-RFLP data retrieved from the amplified bacterial 16S rDNA digested with Cfo I (A) and Msp I (B). Each symbol represents the bacterial community of an individual sample from Riou (square), Medes (triangle) or Scandola (circle), and the colors correspond to different sampling seasons. Sample clusters are based on coordinates determined with the k-means method. Dotted ellipses contain 50% (grey dots) or 95% (dark dots) of the points that contribute to the cluster. The centers of the confidence ellipses are identified with a cross.

    Article Snippet: The restriction reactions were performed for 16 h at 37 °C using a digestion mixture containing 6 µl of the purified PCR products, 1 X reaction buffer (Promega), 0.1 µg µl−1 BSA and 20 U Cfo I or Msp I (Promega) in a final volume of 20 µl.

    Techniques: Amplification, Sampling

    Relative abundances of Msp I TRFs in P. clavata samples analyzed by T-RFLP. As described in Figure 3 , with the restriction enzyme Msp I.

    Journal: PLoS ONE

    Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea

    doi: 10.1371/journal.pone.0057385

    Figure Lengend Snippet: Relative abundances of Msp I TRFs in P. clavata samples analyzed by T-RFLP. As described in Figure 3 , with the restriction enzyme Msp I.

    Article Snippet: The restriction reactions were performed for 16 h at 37 °C using a digestion mixture containing 6 µl of the purified PCR products, 1 X reaction buffer (Promega), 0.1 µg µl−1 BSA and 20 U Cfo I or Msp I (Promega) in a final volume of 20 µl.

    Techniques:

    Agarose gel with different groups of restriction. Line 1 and 11, MW ladder (100 bp); line 2, ARDRA group 1 ( L. plantarum ); line 3, ARDRA group 2 ( P. acidilactici ); line 4, ARDRA group 3 ( W. paramesenteroides ); line 5, ARDRA group 4 ( L. salivarius ); line 6, ARDRA group 5 ( L. ruminis ); line 7, ARDRA group 6 ( L. curvatus ); line 8, ARDRA group 7 ( L. farciminis ); line 9, ARDRA group 8 ( L. mucosae ); line 10, ARDRA group 9 ( E. hirae ). Restriction fragments obtained with each enzyme: (a) Hae III, (b) Msp I, (c) Hinf I.

    Journal: Veterinary Medicine International

    Article Title: Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use

    doi: 10.4061/2010/274987

    Figure Lengend Snippet: Agarose gel with different groups of restriction. Line 1 and 11, MW ladder (100 bp); line 2, ARDRA group 1 ( L. plantarum ); line 3, ARDRA group 2 ( P. acidilactici ); line 4, ARDRA group 3 ( W. paramesenteroides ); line 5, ARDRA group 4 ( L. salivarius ); line 6, ARDRA group 5 ( L. ruminis ); line 7, ARDRA group 6 ( L. curvatus ); line 8, ARDRA group 7 ( L. farciminis ); line 9, ARDRA group 8 ( L. mucosae ); line 10, ARDRA group 9 ( E. hirae ). Restriction fragments obtained with each enzyme: (a) Hae III, (b) Msp I, (c) Hinf I.

    Article Snippet: Three restriction enzymes were used: Hae III, Msp I and Hinf I (Promega).

    Techniques: Agarose Gel Electrophoresis

    UPGMA cluster analysis based on Jaccard similarity coefficients of all samples with a combination of all three enzymes (AciI, MspI, and HaeIII).

    Journal: Applied and Environmental Microbiology

    Article Title: Comparison of Bacteroides-Prevotella 16S rRNA Genetic Markers for Fecal Samples from Different Animal Species

    doi: 10.1128/AEM.71.10.5999-6007.2005

    Figure Lengend Snippet: UPGMA cluster analysis based on Jaccard similarity coefficients of all samples with a combination of all three enzymes (AciI, MspI, and HaeIII).

    Article Snippet: Three different restriction digests were performed on each sample by adding 7 μl of amplified product to 5 U of the following restriction enzymes in separate reactions: AciI (New England BioLabs, Beverly MA), HaeIII (Promega, Madison, WI), and MspI (Promega).

    Techniques:

    Examples of T-RFLP peaks resulting from cow and human fecal samples cut with AciI, MspI, and HaeIII.

    Journal: Applied and Environmental Microbiology

    Article Title: Comparison of Bacteroides-Prevotella 16S rRNA Genetic Markers for Fecal Samples from Different Animal Species

    doi: 10.1128/AEM.71.10.5999-6007.2005

    Figure Lengend Snippet: Examples of T-RFLP peaks resulting from cow and human fecal samples cut with AciI, MspI, and HaeIII.

    Article Snippet: Three different restriction digests were performed on each sample by adding 7 μl of amplified product to 5 U of the following restriction enzymes in separate reactions: AciI (New England BioLabs, Beverly MA), HaeIII (Promega, Madison, WI), and MspI (Promega).

    Techniques:

    Methylation profiles of pig tissue DNA with the primer combinations H-M+TAA/E+AGT. (A) The profile from F-MSAP; (B) Data from (A) quantitated using GeneScan Analysis software; H and M refer to digestion with EcoRI/HpaII and EcoRI/MspI. Lanes 1–7 represent seven tissues, namely muscle, heart, liver, spleen, lung, kidney and stomach; M: GeneScan™ -500ROX™ size standard (represent 200 bp and 220 bp).

    Journal: PLoS ONE

    Article Title: Analysis of DNA Methylation in Various Swine Tissues

    doi: 10.1371/journal.pone.0016229

    Figure Lengend Snippet: Methylation profiles of pig tissue DNA with the primer combinations H-M+TAA/E+AGT. (A) The profile from F-MSAP; (B) Data from (A) quantitated using GeneScan Analysis software; H and M refer to digestion with EcoRI/HpaII and EcoRI/MspI. Lanes 1–7 represent seven tissues, namely muscle, heart, liver, spleen, lung, kidney and stomach; M: GeneScan™ -500ROX™ size standard (represent 200 bp and 220 bp).

    Article Snippet: A total of 30 ug genomic DNA from each tissue was digested separately with EcoRI- HpaII and EcoRI-MspI, the digestion product were electrophoresed on a 0.8% agarose gel in TBE and transferred onto Hybond-N+ membranes (Promega).

    Techniques: Methylation, Software

    Cytosine methylation patterns with the primer combination H-M+TAA/E+AGT. (A) The profile from F-MSAP; (B) The data from (A) quantitated using GeneScan Analysis software; (C) The profile from MSAP using silver stain; H and M refer to digestion with EcoRI/HpaII and EcoRI/MspI, II and III refer to unmethylated, hemi-methylated and fully methylated sites, respectively.

    Journal: PLoS ONE

    Article Title: Analysis of DNA Methylation in Various Swine Tissues

    doi: 10.1371/journal.pone.0016229

    Figure Lengend Snippet: Cytosine methylation patterns with the primer combination H-M+TAA/E+AGT. (A) The profile from F-MSAP; (B) The data from (A) quantitated using GeneScan Analysis software; (C) The profile from MSAP using silver stain; H and M refer to digestion with EcoRI/HpaII and EcoRI/MspI, II and III refer to unmethylated, hemi-methylated and fully methylated sites, respectively.

    Article Snippet: A total of 30 ug genomic DNA from each tissue was digested separately with EcoRI- HpaII and EcoRI-MspI, the digestion product were electrophoresed on a 0.8% agarose gel in TBE and transferred onto Hybond-N+ membranes (Promega).

    Techniques: Methylation, Software, Silver Staining

    The results of Southern blots. (A) Hybridization pattern using probe T04 for the seven tissues; (B) Hybridization pattern using probe T72 for the seven tissues; (C) Hybridization pattern using probe T53 for the seven tissues; H and M indicate the enzyme combination of EcoRI/HpaII and EcoRI/MspI; Lanes 1–7 represent muscle, heart, liver, spleen, lung, kidney and stomach, respectively.

    Journal: PLoS ONE

    Article Title: Analysis of DNA Methylation in Various Swine Tissues

    doi: 10.1371/journal.pone.0016229

    Figure Lengend Snippet: The results of Southern blots. (A) Hybridization pattern using probe T04 for the seven tissues; (B) Hybridization pattern using probe T72 for the seven tissues; (C) Hybridization pattern using probe T53 for the seven tissues; H and M indicate the enzyme combination of EcoRI/HpaII and EcoRI/MspI; Lanes 1–7 represent muscle, heart, liver, spleen, lung, kidney and stomach, respectively.

    Article Snippet: A total of 30 ug genomic DNA from each tissue was digested separately with EcoRI- HpaII and EcoRI-MspI, the digestion product were electrophoresed on a 0.8% agarose gel in TBE and transferred onto Hybond-N+ membranes (Promega).

    Techniques: Hybridization