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  • 99
    New England Biolabs restriction endonuclease mspi
    Nonmetric multidimensional scaling (nMDS) ordination of bacterial community T-RFLP data for 14 samples collected from two DDGS-fed reactors (reactors A and B) over phases I to IV with <t>HaeIII</t> (A) and <t>MspI</t> (B) restriction enzyme digestion. Statistically
    Restriction Endonuclease Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease mspi/product/New England Biolabs
    Average 99 stars, based on 36 article reviews
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    93
    Thermo Fisher fastdigest mspi
    Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains <t>Fermentas</t> O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® <t>MspI</t> restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.
    Fastdigest Mspi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest mspi/product/Thermo Fisher
    Average 93 stars, based on 66 article reviews
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    fastdigest mspi - by Bioz Stars, 2020-05
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    94
    Promega mspi
    UPGMA cluster analysis based on Jaccard similarity coefficients of all samples with a combination of all three enzymes (AciI, <t>MspI,</t> and <t>HaeIII).</t>
    Mspi, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/Promega
    Average 94 stars, based on 188 article reviews
    Price from $9.99 to $1999.99
    mspi - by Bioz Stars, 2020-05
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    99
    New England Biolabs mspi
    Reduced cytosine methylation of centromeric repeats in Bor-4. ( A ) Genomic <t>DNA</t> samples from the indicated genotypes were digested with the isoschizomers HpaII or <t>MspI,</t> and DNA blot hybridization with a 180-bp centromere repeat probe (CEN) was performed. ( B ) The filter shown in A was rehybridized with a 5S rRNA probe (5S rRNA). ( C ) A DNA blot hybridization pattern with the CEN probe after HpaII digestion demonstrates that centromeric repeat arrays hypomethylated in Bor-4 were fully remethylated in F1 hybrids resulting from reciprocal crosses between strains Bor-4 and Ler. The lane labeled Ler + Bor-4 contains a 1:1 mixture of Ler and Bor-4 HpaII-digested genomic DNA and shows the hybridization pattern expected if no remethylation occurred in the F1 hybrids.
    Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/New England Biolabs
    Average 99 stars, based on 2235 article reviews
    Price from $9.99 to $1999.99
    mspi - by Bioz Stars, 2020-05
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    92
    BIORON GmbH mspi
    Reduced cytosine methylation of centromeric repeats in Bor-4. ( A ) Genomic <t>DNA</t> samples from the indicated genotypes were digested with the isoschizomers HpaII or <t>MspI,</t> and DNA blot hybridization with a 180-bp centromere repeat probe (CEN) was performed. ( B ) The filter shown in A was rehybridized with a 5S rRNA probe (5S rRNA). ( C ) A DNA blot hybridization pattern with the CEN probe after HpaII digestion demonstrates that centromeric repeat arrays hypomethylated in Bor-4 were fully remethylated in F1 hybrids resulting from reciprocal crosses between strains Bor-4 and Ler. The lane labeled Ler + Bor-4 contains a 1:1 mixture of Ler and Bor-4 HpaII-digested genomic DNA and shows the hybridization pattern expected if no remethylation occurred in the F1 hybrids.
    Mspi, supplied by BIORON GmbH, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/BIORON GmbH
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    mspi - by Bioz Stars, 2020-05
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    92
    Boehringer Mannheim mspi
    Reduced cytosine methylation of centromeric repeats in Bor-4. ( A ) Genomic <t>DNA</t> samples from the indicated genotypes were digested with the isoschizomers HpaII or <t>MspI,</t> and DNA blot hybridization with a 180-bp centromere repeat probe (CEN) was performed. ( B ) The filter shown in A was rehybridized with a 5S rRNA probe (5S rRNA). ( C ) A DNA blot hybridization pattern with the CEN probe after HpaII digestion demonstrates that centromeric repeat arrays hypomethylated in Bor-4 were fully remethylated in F1 hybrids resulting from reciprocal crosses between strains Bor-4 and Ler. The lane labeled Ler + Bor-4 contains a 1:1 mixture of Ler and Bor-4 HpaII-digested genomic DNA and shows the hybridization pattern expected if no remethylation occurred in the F1 hybrids.
    Mspi, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/Boehringer Mannheim
    Average 92 stars, based on 18 article reviews
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    92
    GE Healthcare mspi
    Reduced cytosine methylation of centromeric repeats in Bor-4. ( A ) Genomic <t>DNA</t> samples from the indicated genotypes were digested with the isoschizomers HpaII or <t>MspI,</t> and DNA blot hybridization with a 180-bp centromere repeat probe (CEN) was performed. ( B ) The filter shown in A was rehybridized with a 5S rRNA probe (5S rRNA). ( C ) A DNA blot hybridization pattern with the CEN probe after HpaII digestion demonstrates that centromeric repeat arrays hypomethylated in Bor-4 were fully remethylated in F1 hybrids resulting from reciprocal crosses between strains Bor-4 and Ler. The lane labeled Ler + Bor-4 contains a 1:1 mixture of Ler and Bor-4 HpaII-digested genomic DNA and shows the hybridization pattern expected if no remethylation occurred in the F1 hybrids.
    Mspi, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/GE Healthcare
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    mspi  (Roche)
    92
    Roche mspi
    Reduced cytosine methylation of centromeric repeats in Bor-4. ( A ) Genomic <t>DNA</t> samples from the indicated genotypes were digested with the isoschizomers HpaII or <t>MspI,</t> and DNA blot hybridization with a 180-bp centromere repeat probe (CEN) was performed. ( B ) The filter shown in A was rehybridized with a 5S rRNA probe (5S rRNA). ( C ) A DNA blot hybridization pattern with the CEN probe after HpaII digestion demonstrates that centromeric repeat arrays hypomethylated in Bor-4 were fully remethylated in F1 hybrids resulting from reciprocal crosses between strains Bor-4 and Ler. The lane labeled Ler + Bor-4 contains a 1:1 mixture of Ler and Bor-4 HpaII-digested genomic DNA and shows the hybridization pattern expected if no remethylation occurred in the F1 hybrids.
    Mspi, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/Roche
    Average 92 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    mspi - by Bioz Stars, 2020-05
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    99
    New England Biolabs ecori mspi
    Reduced cytosine methylation of centromeric repeats in Bor-4. ( A ) Genomic <t>DNA</t> samples from the indicated genotypes were digested with the isoschizomers HpaII or <t>MspI,</t> and DNA blot hybridization with a 180-bp centromere repeat probe (CEN) was performed. ( B ) The filter shown in A was rehybridized with a 5S rRNA probe (5S rRNA). ( C ) A DNA blot hybridization pattern with the CEN probe after HpaII digestion demonstrates that centromeric repeat arrays hypomethylated in Bor-4 were fully remethylated in F1 hybrids resulting from reciprocal crosses between strains Bor-4 and Ler. The lane labeled Ler + Bor-4 contains a 1:1 mixture of Ler and Bor-4 HpaII-digested genomic DNA and shows the hybridization pattern expected if no remethylation occurred in the F1 hybrids.
    Ecori Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori mspi/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
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    Image Search Results


    Nonmetric multidimensional scaling (nMDS) ordination of bacterial community T-RFLP data for 14 samples collected from two DDGS-fed reactors (reactors A and B) over phases I to IV with HaeIII (A) and MspI (B) restriction enzyme digestion. Statistically

    Journal: Applied and Environmental Microbiology

    Article Title: Changing Feeding Regimes To Demonstrate Flexible Biogas Production: Effects on Process Performance, Microbial Community Structure, and Methanogenesis Pathways

    doi: 10.1128/AEM.02320-15

    Figure Lengend Snippet: Nonmetric multidimensional scaling (nMDS) ordination of bacterial community T-RFLP data for 14 samples collected from two DDGS-fed reactors (reactors A and B) over phases I to IV with HaeIII (A) and MspI (B) restriction enzyme digestion. Statistically

    Article Snippet: Briefly, bacterial 16S rRNA genes were subjected to restriction enzyme digestion with either the restriction endonuclease HaeIII or the restriction endonuclease MspI (New England BioLabs, Schwalbach, Germany), whereas mcrA genes were digested with BstNI (New England BioLabs, Schwalbach, Germany).

    Techniques:

    Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.

    Journal: BMC Medical Genetics

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

    doi: 10.1186/1471-2350-13-34

    Figure Lengend Snippet: Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.

    Article Snippet: To identify MTHFR 677 C > T, eNOS +894 G > T and eNOS −786 T > C variants, 1.0 U Fermentas Fast Digest® HinfI restriction enzyme, 1.0 U Fermentas Fast Digest® MspI restriction enzyme and 1.0 U of NEB® BanII restriction enzyme, respectively, were used in separate tubes to avoid errors.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Multiplex Assay

    UPGMA cluster analysis based on Jaccard similarity coefficients of all samples with a combination of all three enzymes (AciI, MspI, and HaeIII).

    Journal: Applied and Environmental Microbiology

    Article Title: Comparison of Bacteroides-Prevotella 16S rRNA Genetic Markers for Fecal Samples from Different Animal Species

    doi: 10.1128/AEM.71.10.5999-6007.2005

    Figure Lengend Snippet: UPGMA cluster analysis based on Jaccard similarity coefficients of all samples with a combination of all three enzymes (AciI, MspI, and HaeIII).

    Article Snippet: Three different restriction digests were performed on each sample by adding 7 μl of amplified product to 5 U of the following restriction enzymes in separate reactions: AciI (New England BioLabs, Beverly MA), HaeIII (Promega, Madison, WI), and MspI (Promega).

    Techniques:

    Examples of T-RFLP peaks resulting from cow and human fecal samples cut with AciI, MspI, and HaeIII.

    Journal: Applied and Environmental Microbiology

    Article Title: Comparison of Bacteroides-Prevotella 16S rRNA Genetic Markers for Fecal Samples from Different Animal Species

    doi: 10.1128/AEM.71.10.5999-6007.2005

    Figure Lengend Snippet: Examples of T-RFLP peaks resulting from cow and human fecal samples cut with AciI, MspI, and HaeIII.

    Article Snippet: Three different restriction digests were performed on each sample by adding 7 μl of amplified product to 5 U of the following restriction enzymes in separate reactions: AciI (New England BioLabs, Beverly MA), HaeIII (Promega, Madison, WI), and MspI (Promega).

    Techniques:

    MDS ordination plot of P. clavata bacterial communities. 2-D scatter plots of T-RFLP profiles from P. clavata colonies sampled at 3 sites in winter and summer from 2007 to 2010 are based on the Dice similarity matrix for the T-RFLP data retrieved from the amplified bacterial 16S rDNA digested with Cfo I (A) and Msp I (B). Each symbol represents the bacterial community of an individual sample from Riou (square), Medes (triangle) or Scandola (circle), and the colors correspond to different sampling seasons. Sample clusters are based on coordinates determined with the k-means method. Dotted ellipses contain 50% (grey dots) or 95% (dark dots) of the points that contribute to the cluster. The centers of the confidence ellipses are identified with a cross.

    Journal: PLoS ONE

    Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea

    doi: 10.1371/journal.pone.0057385

    Figure Lengend Snippet: MDS ordination plot of P. clavata bacterial communities. 2-D scatter plots of T-RFLP profiles from P. clavata colonies sampled at 3 sites in winter and summer from 2007 to 2010 are based on the Dice similarity matrix for the T-RFLP data retrieved from the amplified bacterial 16S rDNA digested with Cfo I (A) and Msp I (B). Each symbol represents the bacterial community of an individual sample from Riou (square), Medes (triangle) or Scandola (circle), and the colors correspond to different sampling seasons. Sample clusters are based on coordinates determined with the k-means method. Dotted ellipses contain 50% (grey dots) or 95% (dark dots) of the points that contribute to the cluster. The centers of the confidence ellipses are identified with a cross.

    Article Snippet: The restriction reactions were performed for 16 h at 37 °C using a digestion mixture containing 6 µl of the purified PCR products, 1 X reaction buffer (Promega), 0.1 µg µl−1 BSA and 20 U Cfo I or Msp I (Promega) in a final volume of 20 µl.

    Techniques: Amplification, Sampling

    Relative abundances of Msp I TRFs in P. clavata samples analyzed by T-RFLP. As described in Figure 3 , with the restriction enzyme Msp I.

    Journal: PLoS ONE

    Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea

    doi: 10.1371/journal.pone.0057385

    Figure Lengend Snippet: Relative abundances of Msp I TRFs in P. clavata samples analyzed by T-RFLP. As described in Figure 3 , with the restriction enzyme Msp I.

    Article Snippet: The restriction reactions were performed for 16 h at 37 °C using a digestion mixture containing 6 µl of the purified PCR products, 1 X reaction buffer (Promega), 0.1 µg µl−1 BSA and 20 U Cfo I or Msp I (Promega) in a final volume of 20 µl.

    Techniques:

    Reduced cytosine methylation of centromeric repeats in Bor-4. ( A ) Genomic DNA samples from the indicated genotypes were digested with the isoschizomers HpaII or MspI, and DNA blot hybridization with a 180-bp centromere repeat probe (CEN) was performed. ( B ) The filter shown in A was rehybridized with a 5S rRNA probe (5S rRNA). ( C ) A DNA blot hybridization pattern with the CEN probe after HpaII digestion demonstrates that centromeric repeat arrays hypomethylated in Bor-4 were fully remethylated in F1 hybrids resulting from reciprocal crosses between strains Bor-4 and Ler. The lane labeled Ler + Bor-4 contains a 1:1 mixture of Ler and Bor-4 HpaII-digested genomic DNA and shows the hybridization pattern expected if no remethylation occurred in the F1 hybrids.

    Journal: Genes & Development

    Article Title: VIM1, a methylcytosine-binding protein required for centromeric heterochromatinization

    doi: 10.1101/gad.1512007

    Figure Lengend Snippet: Reduced cytosine methylation of centromeric repeats in Bor-4. ( A ) Genomic DNA samples from the indicated genotypes were digested with the isoschizomers HpaII or MspI, and DNA blot hybridization with a 180-bp centromere repeat probe (CEN) was performed. ( B ) The filter shown in A was rehybridized with a 5S rRNA probe (5S rRNA). ( C ) A DNA blot hybridization pattern with the CEN probe after HpaII digestion demonstrates that centromeric repeat arrays hypomethylated in Bor-4 were fully remethylated in F1 hybrids resulting from reciprocal crosses between strains Bor-4 and Ler. The lane labeled Ler + Bor-4 contains a 1:1 mixture of Ler and Bor-4 HpaII-digested genomic DNA and shows the hybridization pattern expected if no remethylation occurred in the F1 hybrids.

    Article Snippet: Genomic DNA was digested with HpaII, MspI, or HhaI according to the manufacturer’s (New England Biolabs) instructions.

    Techniques: Methylation, Hybridization, Labeling

    Stable demethylated and variable methylation profiles for the control (A) , ODN-treated (B–D) and GM-CHS (E–G) plants. The figure presents the percentage of the cytosine methylation in the CCGG sites in the flax genome. The genomic DNA was digested by restriction enzymes HpaII and MspI. The amount of non-digested DNA was determined by real-time PCR. The percentage of particular modification was presented for studied plants and control: CCGG, lack of methylation (dark gray); CCmGG, methylation of internal cytosine (light gray); CmCmGG, methylation of both cytosines (middle gray). The site positions were presented according to the CHS2 sequence (the presence of all CCGG sites). Stably demethylated sites: 5′-UTR (−232), non-coding (+217) and coding (+1,606). Variable sites: coding region (+996, +1,219, +1,273). Data constitute the mean value ± SD from at least three independent experiments. The significance of the differences between each mean and control was determined by Student's t -test. Asterisk indicates p

    Journal: Frontiers in Plant Science

    Article Title: Oligodeoxynucleotides Can Transiently Up- and Downregulate CHS Gene Expression in Flax by Changing DNA Methylation in a Sequence-Specific Manner

    doi: 10.3389/fpls.2017.00755

    Figure Lengend Snippet: Stable demethylated and variable methylation profiles for the control (A) , ODN-treated (B–D) and GM-CHS (E–G) plants. The figure presents the percentage of the cytosine methylation in the CCGG sites in the flax genome. The genomic DNA was digested by restriction enzymes HpaII and MspI. The amount of non-digested DNA was determined by real-time PCR. The percentage of particular modification was presented for studied plants and control: CCGG, lack of methylation (dark gray); CCmGG, methylation of internal cytosine (light gray); CmCmGG, methylation of both cytosines (middle gray). The site positions were presented according to the CHS2 sequence (the presence of all CCGG sites). Stably demethylated sites: 5′-UTR (−232), non-coding (+217) and coding (+1,606). Variable sites: coding region (+996, +1,219, +1,273). Data constitute the mean value ± SD from at least three independent experiments. The significance of the differences between each mean and control was determined by Student's t -test. Asterisk indicates p

    Article Snippet: The DNA was incubated with restriction enzymes MspI and HpaII for at least 3 h (restriction enzymes MspI and HpaII (New England Biolabs) differ in sensitivity to cytosine methylation).

    Techniques: Methylation, Real-time Polymerase Chain Reaction, Modification, Sequencing, Stable Transfection

    Control lambda DNA analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated lambda DNAs digested with HpaII and MspI. ND: lambda DNA not digested. b GDMI values of unmethylated and methylated lambda DNAs. The values represent the main of three independent triplicate experiments with standard error mean

    Journal: Age

    Article Title: Global DNA methylation in old subjects is correlated with frailty

    doi: 10.1007/s11357-011-9216-6

    Figure Lengend Snippet: Control lambda DNA analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated lambda DNAs digested with HpaII and MspI. ND: lambda DNA not digested. b GDMI values of unmethylated and methylated lambda DNAs. The values represent the main of three independent triplicate experiments with standard error mean

    Article Snippet: One hundred nanograms of methylated and unmethylated lambda DNA were separately incubated with 5 U of HpaII and MspI restriction endonucleases (New England Biolabs) at 37°C overnight and successively at 65°C for 20 min to inactivate the endonucleases.

    Techniques: Lambda DNA Preparation, Staining, Agarose Gel Electrophoresis, Methylation

    Control human DNA analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated human DNAs and a mixture of equal amount of unmethylated and methylated human DNAs digested with HpaII and MspI. ND human DNA not digested. b GDMI values of unmethylated, methylated and the mixture of unmethylated and methylated human DNAs. The values represent the main of three independent triplicate experiments with standard error mean

    Journal: Age

    Article Title: Global DNA methylation in old subjects is correlated with frailty

    doi: 10.1007/s11357-011-9216-6

    Figure Lengend Snippet: Control human DNA analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated human DNAs and a mixture of equal amount of unmethylated and methylated human DNAs digested with HpaII and MspI. ND human DNA not digested. b GDMI values of unmethylated, methylated and the mixture of unmethylated and methylated human DNAs. The values represent the main of three independent triplicate experiments with standard error mean

    Article Snippet: One hundred nanograms of methylated and unmethylated lambda DNA were separately incubated with 5 U of HpaII and MspI restriction endonucleases (New England Biolabs) at 37°C overnight and successively at 65°C for 20 min to inactivate the endonucleases.

    Techniques: Staining, Agarose Gel Electrophoresis, Methylation