nti mlc2 (Proteintech)
Structured Review
Nti Mlc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mlc2/Myosin+Light+Chain+2%2FMLC-2V+Antibody/pm41933938-251-40-42
Average 94 stars, based on 205 article reviews
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Synthesized:Article Title: RHOA lactylation at oncogenic hotspots promotes oncogenic activity and protein stabilization. Article Snippet: Human KCTD13 and LDHA gene were amplified from MDA-MB231 cDNA library and subcloned into pLVX-Puro vector. pCMV-3×FLAG-USP9X(human)-Neo (P56773) and pCMV-USP33(human)-3×Myc-Neo(P71806) were obtained from MiaoLingBio, China. .. The DNA fragments of Mb-Pyl Klacr-RS/Pyl-tRNA pair were synthesized by Beijing Tsingke Biotech Co., Ltd. Antibodies against RHOA and Article Title: RHOA lactylation at oncogenic hotspots promotes oncogenic activity and protein stabilization Article Snippet: Human KCTD13 and LDHA gene were amplified from MDA-MB-231 cDNA library and subcloned into pLVX-Puro vector. pCMV-3 × FLAG-USP9X(human)-Neo ( P56773 ) and pCMV-USP33(human)−3 × Myc-Neo( P71806 ) were obtained from MiaoLingBio, China. .. The DNA fragments of Mb-Pyl Klacr-RS/Pyl-tRNA pair were synthesized by Beijing Tsingke Biotech Co., Ltd. Antibodies against RHOA and Membrane:Article Title: CapG promoted nasopharyngeal carcinoma cell motility involving Rho motility pathway independent of ROCK Article Snippet: Denatured protein was separated via 10% SDS polyacrylamide gel electrophoresis and transferred to PVDF membrane (0.22 μm, Merck Millipore, MA, USA). .. Membrane was blocked with TBST solution (20 mM Tris, 137 mM NaCl, 0.1% Triton X-100, pH 7.6 ± 0.1) containing 5% nonfat milk for 1 h at room temperature, followed by incubation with specific primary antibodies at 4 °C for 16 h. The primary antibodies included alpha tubulin (1:2000; ab52866, Abcam, Cambridge, UK), CapG (1:1000; ab155688, Abcam, Cambridge, UK), Article Title: CapG promoted nasopharyngeal carcinoma cell motility involving Rho motility pathway independent of ROCK. Article Snippet: Denatured protein was separated via 10% SDS polyacrylamide gel electrophoresis and transferred to PVDF membrane (0.22 μm, Merck Millipore, MA, USA). .. Membrane was blocked with TBST solution (20 mM Tris, 137 mM NaCl, 0.1% Triton X-100, pH 7.6 ± 0.1) containing 5% nonfat milk for 1 h at room temperature, followed by incubation with specific primary antibodies at 4 °C for 16 h. The primary antibodies included alpha tubulin (1:2000; ab52866, Abcam, Cambridge, UK), CapG (1:1000; ab155688, Abcam, Cambridge, UK), Incubation:Article Title: CapG promoted nasopharyngeal carcinoma cell motility involving Rho motility pathway independent of ROCK Article Snippet: Denatured protein was separated via 10% SDS polyacrylamide gel electrophoresis and transferred to PVDF membrane (0.22 μm, Merck Millipore, MA, USA). .. Membrane was blocked with TBST solution (20 mM Tris, 137 mM NaCl, 0.1% Triton X-100, pH 7.6 ± 0.1) containing 5% nonfat milk for 1 h at room temperature, followed by incubation with specific primary antibodies at 4 °C for 16 h. The primary antibodies included alpha tubulin (1:2000; ab52866, Abcam, Cambridge, UK), CapG (1:1000; ab155688, Abcam, Cambridge, UK), Article Title: CapG promoted nasopharyngeal carcinoma cell motility involving Rho motility pathway independent of ROCK. Article Snippet: Denatured protein was separated via 10% SDS polyacrylamide gel electrophoresis and transferred to PVDF membrane (0.22 μm, Merck Millipore, MA, USA). .. Membrane was blocked with TBST solution (20 mM Tris, 137 mM NaCl, 0.1% Triton X-100, pH 7.6 ± 0.1) containing 5% nonfat milk for 1 h at room temperature, followed by incubation with specific primary antibodies at 4 °C for 16 h. The primary antibodies included alpha tubulin (1:2000; ab52866, Abcam, Cambridge, UK), CapG (1:1000; ab155688, Abcam, Cambridge, UK), Article Title: The Hippo pathway links adipocyte plasticity to adipose tissue fibrosis Article Snippet: .. Membranes were blotted with primary antibodies against HSP90 (Santa Cruz, sc-13119, 1:10,000), α-Tubulin (Sigma, T6199, 1:10,000), Lamin B1 (Proteintech, 66095-1-Ig, 1:10,000), LATS1 (Cell Signaling, 3477, 1:1000), LATS2 (Bethyl Laboratories, A300-479A, 1:1000), YAP (Cell Signaling, 4912 or 14074, 1:1000), TAZ (Cell Signaling, 4883 or 83669, 1:1000), p-YAP (Ser 112) (Cell Signaling, 4911), p-TAZ (Ser 89) (Cell Signaling, 59971, 1:1000), HSL (Cell Signaling, 4107, 1:1000), Perilipin 1 (Vala Sciences, 4854, 1:10,000), SMAD2/3 (Cell Signaling, 8685, 1:1000), p-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (Cell Signaling, 8828, 1:1000), αSMA (Cell Signaling, 19245, 1:5000), FLAG (Abmart, M20008, 1:5000), Caspase 3 (Cell Signaling, 9662,1:1000), MST1 (Cell Signaling, 3682, 1:1000), MST2 (Cell Signaling, 3952, 1:1000), p-SAPK/JNK (Thr183/Tyr185) (Cell Signaling, 4668, 1:1000), SAPK/JNK (Cell Signaling, 9252, 1:1000), p-AKT (Ser473) (Cell Signaling, 4060, 1:1000), AKT (Cell Signaling, 9272, 1:1000), p-p38 MAPK (Thr180/Tyr182) (Cell Signaling, 4511, 1:1000), p38 MAPK (Cell Signaling, 8690, 1:1000), |
