Review



2 mercaptoethanol  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Bio-Rad 2 mercaptoethanol
    2 Mercaptoethanol, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 4796 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mercaptoethanol/2-Mercaptoethanol/pmc13101689-160-14-15
    Average 97 stars, based on 4796 article reviews
    2 mercaptoethanol - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    SDS Page:

    Article Title: CRISPR inhibition of activity-dependent Arc expression in the adult mouse brain has limited effects on plasticity in visual cortex and nucleus accumbens
    Article Snippet: Cells were washed 3X with PBS and incubated with goat anti-rabbit Cy 2 (1:500, Jackson Immuno, 305-227-003) for 3 hr at room temperature (RT) prior to rising with PBS, labeling nuclei with Hoechst (1:1000 Thermo Fisher Scientific, H1399) and mounting in Vectashield (VectorLabs H1000-10). .. 2x Laemmli Sample Buffer (Bio-Rad, Cat# 1610737) and 2-mercaptoethanol were added to each sample and boiled for 10 min prior to separation by SDS-PAGE on a 12% gel and transfer to nitrocellulose. ..

    Article Title: Thermoreversible cell-derived extracellular matrix only hydrogel (CEOgel): Development, characterization, and applications
    Article Snippet: .. For SDS-PAGE analysis, the cENF solution was mixed with Laemmli sample buffer (Bio-Rad, Hercules, CA, USA) containing 10% 2-mercaptoethanol and heated at 98 °C for 5 min. After cooling, 30 μg of protein per lane was loaded onto a 4–15% gradient gel (Bio-Rad). .. A standard protein marker (ExcelBandTM 3-Color Extra Range Protein Marker, SMOBIO Technology, Hsinchu City, Taiwan) was loaded in a separate lane.

    Membrane:

    Article Title: Stromal Gasdermin D-mediated Pyroptosis Drives Maladaptive CD4⁺ T-cell Remodeling in Tet2-Deficient Hematopoiesis
    Article Snippet: .. Lysates were reduced with 2-mercaptoethanol and resolved by SDS–PAGE using 4-12% gradient gels (Bio-Rad), followed by wet transfer to a membrane. .. Membranes were probed with an anti-N-terminal GSDMD antibody (ab219800; Abcam) and HSC70 (Santa Cruz, #sc-7298) as a loading control.

    other:

    Article Title: Systemic Propagation of STING Signalling via Generation of Large Extracellular Vesicles.
    Article Snippet: Cells were cultured in DMEM (Life Technologies, 1-965-118) supplemented with 10% FBS (Life Technologies, 16140- 71) and 1% penicillin-streptomycin (Life Technologies, 15-140- 22) and were regularly tested for mycoplasma (Applied Biologial Materials, G328).

    Article Title: Effects of novel protein extraction methods on structure-functional properties and protein quality of fava bean protein isolates: A comparative study.
    Article Snippet: Laemmli sample buffer, 2-mercaptoethanol, Precision Plus protein standard (10-250 kDa), Coomassie Blue R-250, and 4-15% Mini-PROTEAN® TGXTM precast protein gels were purchased from Bio-Rad Laboratories Ltd (Mississauga, ON, Canada).

    Blocking Assay:

    Article Title: Inhibition of endolysosomal two-pore channel 2 (TPC2) induces osteoblast differentiation and matrix mineralization while targeting autophagy.
    Article Snippet: Total protein content of lysates was measured with Bicinchoninic acid assay (BCA) (Pierce, Rockford, IL, USA) according to the standard protocol. .. Samples were reduced with 2-mercaptoethanol on heat block for 5 min at 95 °C and later, the proteins were separated using SDSPAGE under reducing conditions using 10% and 15% polyacrylamide gels and blotted onto nitrocellulose or PVDF (Amersham Bioscience, Little Chalfont, UK) membranes using a trans blot electrophoresis apparatus (Bio-Rad, USA) for 2 h at 70 V. After transferring, the membranes were incubated in the blocking buffer (5% nonfat dry milk in T-TBS) and then incubated with primary antibody overnight at 4 °C. .. After three times washing with T-TBS, membranes were incubated with the peroxidase-conjugated secondary antibody (Jackson Laboratories, Ann Arbor, MI, USA) for 60 min on the shaker at room temperature.

    Electrophoresis:

    Article Title: Inhibition of endolysosomal two-pore channel 2 (TPC2) induces osteoblast differentiation and matrix mineralization while targeting autophagy.
    Article Snippet: Total protein content of lysates was measured with Bicinchoninic acid assay (BCA) (Pierce, Rockford, IL, USA) according to the standard protocol. .. Samples were reduced with 2-mercaptoethanol on heat block for 5 min at 95 °C and later, the proteins were separated using SDSPAGE under reducing conditions using 10% and 15% polyacrylamide gels and blotted onto nitrocellulose or PVDF (Amersham Bioscience, Little Chalfont, UK) membranes using a trans blot electrophoresis apparatus (Bio-Rad, USA) for 2 h at 70 V. After transferring, the membranes were incubated in the blocking buffer (5% nonfat dry milk in T-TBS) and then incubated with primary antibody overnight at 4 °C. .. After three times washing with T-TBS, membranes were incubated with the peroxidase-conjugated secondary antibody (Jackson Laboratories, Ann Arbor, MI, USA) for 60 min on the shaker at room temperature.

    Transferring:

    Article Title: Inhibition of endolysosomal two-pore channel 2 (TPC2) induces osteoblast differentiation and matrix mineralization while targeting autophagy.
    Article Snippet: Total protein content of lysates was measured with Bicinchoninic acid assay (BCA) (Pierce, Rockford, IL, USA) according to the standard protocol. .. Samples were reduced with 2-mercaptoethanol on heat block for 5 min at 95 °C and later, the proteins were separated using SDSPAGE under reducing conditions using 10% and 15% polyacrylamide gels and blotted onto nitrocellulose or PVDF (Amersham Bioscience, Little Chalfont, UK) membranes using a trans blot electrophoresis apparatus (Bio-Rad, USA) for 2 h at 70 V. After transferring, the membranes were incubated in the blocking buffer (5% nonfat dry milk in T-TBS) and then incubated with primary antibody overnight at 4 °C. .. After three times washing with T-TBS, membranes were incubated with the peroxidase-conjugated secondary antibody (Jackson Laboratories, Ann Arbor, MI, USA) for 60 min on the shaker at room temperature.

    Incubation:

    Article Title: Inhibition of endolysosomal two-pore channel 2 (TPC2) induces osteoblast differentiation and matrix mineralization while targeting autophagy.
    Article Snippet: Total protein content of lysates was measured with Bicinchoninic acid assay (BCA) (Pierce, Rockford, IL, USA) according to the standard protocol. .. Samples were reduced with 2-mercaptoethanol on heat block for 5 min at 95 °C and later, the proteins were separated using SDSPAGE under reducing conditions using 10% and 15% polyacrylamide gels and blotted onto nitrocellulose or PVDF (Amersham Bioscience, Little Chalfont, UK) membranes using a trans blot electrophoresis apparatus (Bio-Rad, USA) for 2 h at 70 V. After transferring, the membranes were incubated in the blocking buffer (5% nonfat dry milk in T-TBS) and then incubated with primary antibody overnight at 4 °C. .. After three times washing with T-TBS, membranes were incubated with the peroxidase-conjugated secondary antibody (Jackson Laboratories, Ann Arbor, MI, USA) for 60 min on the shaker at room temperature.



    Similar Products

    2 mercaptoethanol  (Thermo Fisher)
    99
    Thermo Fisher 2 mercaptoethanol
    2 Mercaptoethanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mercaptoethanol/2-Mercaptoethanol%2C+98%2B%25/pmc13170521-241-31-34
    Average 99 stars, based on 1 article reviews
    2 mercaptoethanol - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    mercaptoethanol  (Thermo Fisher)
    99
    Thermo Fisher mercaptoethanol
    Mercaptoethanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mercaptoethanol/2-Mercaptoethanol%2C+98%2B%25/pm42366241-102-51-48
    Average 99 stars, based on 1 article reviews
    mercaptoethanol - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    β mercaptoethanol  (Amresco)
    86
    Amresco β mercaptoethanol
    β Mercaptoethanol, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mercaptoethanol/mercaptoethanol+%CE%B2/pmc13264271-39-0-2
    Average 86 stars, based on 1 article reviews
    β mercaptoethanol - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    2 mercaptoethanol  (Fisher Scientific)
    86
    Fisher Scientific 2 mercaptoethanol
    2 Mercaptoethanol, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mercaptoethanol/mercaptoethanol+%CE%B2/pmc13217883-41-0-4
    Average 86 stars, based on 1 article reviews
    2 mercaptoethanol - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    2 mercaptoethanol  (Bio-Rad)
    97
    Bio-Rad 2 mercaptoethanol
    2 Mercaptoethanol, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mercaptoethanol/2-Mercaptoethanol/pmc13101689-160-14-15
    Average 97 stars, based on 1 article reviews
    2 mercaptoethanol - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results