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Proteintech mdm2
Mdm2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdm2/MDM2+Antibody/pm42781876-81-5-21
Average 96 stars, based on 191 article reviews
mdm2 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Blocking Assay:

Article Title: Extracellular Vesicle‐Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood‐Tumour Barrier Formation
Article Snippet: The separated proteins were then transferred onto a PVDF membrane (Millipore, Massachusetts, USA) via the Bio‐Rad Trans‐Blot Turbo transfer apparatus (USA). .. Following blocking with 5% non‐fat dry milk in TBST, the membrane was incubated overnight at 4°C with primary antibodies against VEGFR2 (1:1000, CST, USA), EGFR (1:1000, CST, USA), NCL (1:1000, Proteintech, CHN), TSG101 (1:500, Abclonal, CHN), CD63 (1:500, Proteintech, CHN), MDM2 (1:1000, Proteintech, CHN), Na + /K + ‐ATPases (1:1000, Proteintech, CHN) and ACTIN (1:2000, Abclonal, CHN). ..

Article Title: Extracellular Vesicle-Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood-Tumour Barrier Formation.
Article Snippet: The separated proteins were then transferred onto a PVDF membrane (Millipore, Massachusetts, USA) via the Bio-Rad Trans-Blot Turbo transfer apparatus (USA). .. Following blocking with 5% non-fat dry milk in TBST, the membrane was incubated overnight at 4◦C with primary antibodies against VEGFR2 (1:1000, CST, USA), EGFR (1:1000, CST, USA), NCL (1:1000, Proteintech, CHN), TSG101 (1:500, Abclonal, CHN), CD63 (1:500, Proteintech, CHN), MDM2 (1:1000, Proteintech, CHN), Na+ /K+ -ATPases (1:1000, Proteintech, CHN) and ACTIN (1:2000, Abclonal, CHN). ..

Membrane:

Article Title: Extracellular Vesicle‐Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood‐Tumour Barrier Formation
Article Snippet: The separated proteins were then transferred onto a PVDF membrane (Millipore, Massachusetts, USA) via the Bio‐Rad Trans‐Blot Turbo transfer apparatus (USA). .. Following blocking with 5% non‐fat dry milk in TBST, the membrane was incubated overnight at 4°C with primary antibodies against VEGFR2 (1:1000, CST, USA), EGFR (1:1000, CST, USA), NCL (1:1000, Proteintech, CHN), TSG101 (1:500, Abclonal, CHN), CD63 (1:500, Proteintech, CHN), MDM2 (1:1000, Proteintech, CHN), Na + /K + ‐ATPases (1:1000, Proteintech, CHN) and ACTIN (1:2000, Abclonal, CHN). ..

Article Title: Extracellular Vesicle-Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood-Tumour Barrier Formation.
Article Snippet: The separated proteins were then transferred onto a PVDF membrane (Millipore, Massachusetts, USA) via the Bio-Rad Trans-Blot Turbo transfer apparatus (USA). .. Following blocking with 5% non-fat dry milk in TBST, the membrane was incubated overnight at 4◦C with primary antibodies against VEGFR2 (1:1000, CST, USA), EGFR (1:1000, CST, USA), NCL (1:1000, Proteintech, CHN), TSG101 (1:500, Abclonal, CHN), CD63 (1:500, Proteintech, CHN), MDM2 (1:1000, Proteintech, CHN), Na+ /K+ -ATPases (1:1000, Proteintech, CHN) and ACTIN (1:2000, Abclonal, CHN). ..

Incubation:

Article Title: Extracellular Vesicle‐Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood‐Tumour Barrier Formation
Article Snippet: The separated proteins were then transferred onto a PVDF membrane (Millipore, Massachusetts, USA) via the Bio‐Rad Trans‐Blot Turbo transfer apparatus (USA). .. Following blocking with 5% non‐fat dry milk in TBST, the membrane was incubated overnight at 4°C with primary antibodies against VEGFR2 (1:1000, CST, USA), EGFR (1:1000, CST, USA), NCL (1:1000, Proteintech, CHN), TSG101 (1:500, Abclonal, CHN), CD63 (1:500, Proteintech, CHN), MDM2 (1:1000, Proteintech, CHN), Na + /K + ‐ATPases (1:1000, Proteintech, CHN) and ACTIN (1:2000, Abclonal, CHN). ..

Article Title: USP15 promotes proliferation, migration, and apoptosis resistance of pulmonary arterial smooth muscle cells by targeting MDM2.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: Extracellular Vesicle-Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood-Tumour Barrier Formation.
Article Snippet: The separated proteins were then transferred onto a PVDF membrane (Millipore, Massachusetts, USA) via the Bio-Rad Trans-Blot Turbo transfer apparatus (USA). .. Following blocking with 5% non-fat dry milk in TBST, the membrane was incubated overnight at 4◦C with primary antibodies against VEGFR2 (1:1000, CST, USA), EGFR (1:1000, CST, USA), NCL (1:1000, Proteintech, CHN), TSG101 (1:500, Abclonal, CHN), CD63 (1:500, Proteintech, CHN), MDM2 (1:1000, Proteintech, CHN), Na+ /K+ -ATPases (1:1000, Proteintech, CHN) and ACTIN (1:2000, Abclonal, CHN). ..

Cell-Signaling:

Article Title: IGK Suppresses Keloid Fibroblast Activation Through Modulation of MDM2 Expression and p53 Signaling.
Article Snippet: Immune complexes were identified using Immobilon Western Chemiluminescence HRP Substrate, and images were captured with a Bio‐Rad ChemiDoc XRS+ system. .. Antibodies against p53 (Cat#2527) and MDM2 were obtained from Cell Signaling Technology, and the GAPDH Polyclonal antibody (Cat#10494‐1‐AP) was sourced from Proteintech. ..

Article Title: Ubiquitin ligase Nedd4 regulates the abundance and toxicity of mutant huntingtin
Article Snippet: .. The following antibodies were used for Western blotting: Nedd4 (07-049, EMD Millipore), Nedd4 (21698-1-AP, Proteintech), GFP (G1544, Sigma), GFP (632381, TaKaRa), GFP (GTX-113617, Genetex), T7-Tag (#6885, Cell Signaling Technology), huntingtin (MAB5490, MAB5492, MAB2166, MAB5374, MAB1574, MilliporeSigma), huntingtin (BML-PW0595A, Enzo), Huntingtin (ab109115, Abcam), HA- Tag (#3724, Cell Signaling Technology), HA-Tag (MMS-101P, Covance), HA-tag (66006-2-lg, Proteintech), α-tubulin (T5168, Sigma), p62 (18420-1-AP, Proteintech), p62 (P0067, Sigma), p62 (ab56416, Abcam), Ub (P4D1) (sc-8017, Santa Cruz), GAPDH (MAB374, MilliporeSigma), GAPDH (60004-1-lg, Proteintech), β-actin (ab6276, Abcam), Beclin-1 (#3738, Cell Signaling Technology), phospho-S6 (Ser235/236, #2211, Cell Signaling Technology), S6 (#2217, Cell Signaling Technology), Mdm2 (sc-965, Santa Cruz), Mdm2 (27883-1-AP, Proteintech), p53 (21891-1-AP, Proteintech), Vinculin (MAB3574, Millipore), and HRP-conjugated goat anti-rabbit and goat anti-mouse IgG (H+L) secondary antibodies (111-035-144 and 115-035-146, Jackson ImmunoResearch Laboratories). .. The following antibodies were used for IP: GFP (AFP5002, Qbiogene), GFP (G1544, Sigma), and huntingtin (MAB5490, MilliporeSigma).

Western Blot:

Article Title: Ubiquitin ligase Nedd4 regulates the abundance and toxicity of mutant huntingtin
Article Snippet: .. The following antibodies were used for Western blotting: Nedd4 (07-049, EMD Millipore), Nedd4 (21698-1-AP, Proteintech), GFP (G1544, Sigma), GFP (632381, TaKaRa), GFP (GTX-113617, Genetex), T7-Tag (#6885, Cell Signaling Technology), huntingtin (MAB5490, MAB5492, MAB2166, MAB5374, MAB1574, MilliporeSigma), huntingtin (BML-PW0595A, Enzo), Huntingtin (ab109115, Abcam), HA- Tag (#3724, Cell Signaling Technology), HA-Tag (MMS-101P, Covance), HA-tag (66006-2-lg, Proteintech), α-tubulin (T5168, Sigma), p62 (18420-1-AP, Proteintech), p62 (P0067, Sigma), p62 (ab56416, Abcam), Ub (P4D1) (sc-8017, Santa Cruz), GAPDH (MAB374, MilliporeSigma), GAPDH (60004-1-lg, Proteintech), β-actin (ab6276, Abcam), Beclin-1 (#3738, Cell Signaling Technology), phospho-S6 (Ser235/236, #2211, Cell Signaling Technology), S6 (#2217, Cell Signaling Technology), Mdm2 (sc-965, Santa Cruz), Mdm2 (27883-1-AP, Proteintech), p53 (21891-1-AP, Proteintech), Vinculin (MAB3574, Millipore), and HRP-conjugated goat anti-rabbit and goat anti-mouse IgG (H+L) secondary antibodies (111-035-144 and 115-035-146, Jackson ImmunoResearch Laboratories). .. The following antibodies were used for IP: GFP (AFP5002, Qbiogene), GFP (G1544, Sigma), and huntingtin (MAB5490, MilliporeSigma).

Article Title: Ubiquitin ligase Nedd4 regulates the abundance and toxicity of mutant huntingtin.
Article Snippet: .. The following antibodies were used for Western blotting: Nedd4 (07-049, EMD Millipore), Nedd4 (21698-1-AP, Proteintech), GFP (G1544, Sigma), GFP (632381, TaKaRa), GFP (GTX-113617, Genetex), T7-Tag (#6885, Cell Signaling Technology), huntingtin (MAB5490, MAB5492, MAB2166, MAB5374, MAB1574, MilliporeSigma), huntingtin (BML-PW0595A, Enzo), Huntingtin (ab109115, Abcam), HA- Tag (#3724, Cell Signaling Technology), HA-Tag (MMS-101P, Covance), HA-tag (66006-2-lg, Proteintech), α-tubulin (T5168, Sigma), p62 (18420-1-AP, Proteintech), p62 (P0067, Sigma), p62 (ab56416, Abcam), Ub (P4D1) (sc-8017, Santa Cruz), GAPDH (MAB374, MilliporeSigma), GAPDH (60004-1-lg, Proteintech), β-actin (ab6276, Abcam), Beclin-1 (#3738, Cell Signaling Technology), phospho-S6 (Ser235/236, #2211, Cell Signaling Technology), S6 (#2217, Cell Signaling Technology), Mdm2 (sc-965, Santa Cruz), Mdm2 (27883-1-AP, Proteintech), p53 (21891-1-AP, Proteintech), Vinculin (MAB3574, Millipore), and HRP-conjugated goat anti-rabbit and goat anti-mouse IgG (H+L) secondary antibodies (111-035-144 and 115-035-146, Jackson ImmunoResearch Laboratories). .. The following antibodies were used for IP: GFP (AFP5002, Qbiogene), GFP (G1544, Sigma), and huntingtin (MAB5490, MilliporeSigma).

Hemagglutination Assay:

Article Title: Ubiquitin ligase Nedd4 regulates the abundance and toxicity of mutant huntingtin
Article Snippet: .. The following antibodies were used for Western blotting: Nedd4 (07-049, EMD Millipore), Nedd4 (21698-1-AP, Proteintech), GFP (G1544, Sigma), GFP (632381, TaKaRa), GFP (GTX-113617, Genetex), T7-Tag (#6885, Cell Signaling Technology), huntingtin (MAB5490, MAB5492, MAB2166, MAB5374, MAB1574, MilliporeSigma), huntingtin (BML-PW0595A, Enzo), Huntingtin (ab109115, Abcam), HA- Tag (#3724, Cell Signaling Technology), HA-Tag (MMS-101P, Covance), HA-tag (66006-2-lg, Proteintech), α-tubulin (T5168, Sigma), p62 (18420-1-AP, Proteintech), p62 (P0067, Sigma), p62 (ab56416, Abcam), Ub (P4D1) (sc-8017, Santa Cruz), GAPDH (MAB374, MilliporeSigma), GAPDH (60004-1-lg, Proteintech), β-actin (ab6276, Abcam), Beclin-1 (#3738, Cell Signaling Technology), phospho-S6 (Ser235/236, #2211, Cell Signaling Technology), S6 (#2217, Cell Signaling Technology), Mdm2 (sc-965, Santa Cruz), Mdm2 (27883-1-AP, Proteintech), p53 (21891-1-AP, Proteintech), Vinculin (MAB3574, Millipore), and HRP-conjugated goat anti-rabbit and goat anti-mouse IgG (H+L) secondary antibodies (111-035-144 and 115-035-146, Jackson ImmunoResearch Laboratories). .. The following antibodies were used for IP: GFP (AFP5002, Qbiogene), GFP (G1544, Sigma), and huntingtin (MAB5490, MilliporeSigma).

Colorimetric Assay:

Article Title: Magnetic traction and molecular lock-key: Nano-intelligent nanobot overcomes glioblastoma ferroptosis resistance to activate ferroptosis-immune
Article Snippet: RNA interference (RNAi) technology, which silences disease-causing genes, offers a novel therapeutic approach for glioblastoma (GBM).. However, the clinical translation of small interfering RNA (siRNA) faces challenges such as low delivery efficiency, rapid renal clearance, and endosomal/lysosomal entrapment.. This study developed a dual-targeted nanoplatform FZMT, enable precise siRNA delivery while synergistically inducing ferroptosis and antitumor immunity.



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