mdc1 (Proteintech)
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93
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Proteintech
mdc1
Mdc1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdc1/MDC1+Antibody/pmc12906238-48-1-9
Average 93 stars, based on 16 article reviews
Mdc1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdc1/MDC1+Antibody/pmc12906238-48-1-9
Average 93 stars, based on 16 article reviews
mdc1 - by Bioz Stars,
2026-10
93/100 stars
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Immunostaining:Article Title: Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage. Article Snippet: .. After fixed for 15 min in 4% formaldehyde, cells were washed in PBS, permeabilized for 5 min in PBS containing 0.5% Triton-X100, washed twice in PBS, blocked for 1 h in 3% BSA/0.2% Tween-20 in PBS, followed by immunostaining with anti-γ-H2AX (NBP100-121, HUABio) or Article Title: Topoisomerase I Inhibition in ETV4‐overexpressed Non‐Small Cell Lung Cancer Promotes Replication and Transcription Mediated R‐Loop Accumulation and DNA Damage Article Snippet: .. After fixed for 15 min in 4% formaldehyde, cells were washed in PBS, permeabilized for 5 min in PBS containing 0.5% Triton‐X100, washed twice in PBS, blocked for 1 h in 3% BSA/0.2% Tween‐20 in PBS, followed by immunostaining with anti‐γ‐H2AX (NBP100‐121, HUABio) or Immunohistochemistry:Article Title: HTATSF1-Nucleated PARylation and Phosphorylation Cascade Dictates Homologous Recombination and Modulates Chemotherapeutic Response of Breast Carcinoma Article Snippet: State Key Laboratory of Experimental Hematology, The Province and Ministry Cosponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University Cancer Institute and Hospital, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin 300070, China; State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Key Laboratory of Medical Data Analysis and Statistical Research of Tianjin, Nankai University, 300353, Tianjin, China; Core Facilities Center, Capital Medical University, 100069, Beijing, China; Produced:Article Title: HTATSF1-Nucleated PARylation and Phosphorylation Cascade Dictates Homologous Recombination and Modulates Chemotherapeutic Response of Breast Carcinoma Article Snippet: State Key Laboratory of Experimental Hematology, The Province and Ministry Cosponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University Cancer Institute and Hospital, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin 300070, China; State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Key Laboratory of Medical Data Analysis and Statistical Research of Tianjin, Nankai University, 300353, Tianjin, China; Core Facilities Center, Capital Medical University, 100069, Beijing, China; Nucleic Acid Electrophoresis:Article Title: NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors Article Snippet: Cells were harvested in phosphate buffer saline (PBS) (4°C), and cell pellets were lysed with NETN buffer containing complete protease inhibitor cocktail (Roche, Germany) and phosphatase inhibitor cocktail (Cwbiotech, Beijing, China). .. Protein lysates were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blotting assays were performed using the antibodies specific to NRDE2 (1:1,000; 24968-1-AP, Proteintech, China), ATM (1:1,000; 2873T, Cell Signaling Technology, USA), ATR (1:1,000; 2790S, Cell Signaling Technology, USA), CHK1 (1:1,000; 2360S, Cell Signaling Technology, USA), CHK2 (1:1,000; 3440S, Cell Signaling Technology, USA), CK2A1 (1:2,000; 10992-1-AP, Proteintech, China), CK2A2 (1:2,000; 10606-1-AP, Proteintech, China), CK2B (1:2,000; NBP1-06514, Nouvs Biologicals, USA), CK2 phosphorylation (1:1,000; 8738, Cell Signaling Technology, USA), Flag (1:2,000; F3165, Sigma, USA), HA (1:2,000; M180-3, MBL, Japan), Histone H3 (1:1,000; 17168-1-AP, Proteintech, China), Article Title: NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors. Article Snippet: Immunoblotting assays Cells were harvested in phosphate buffer saline (PBS) (4 C), and cell pellets were lysed with NETN buffer containing complete protease inhibitor cocktail (Roche, Germany) and phosphatase inhibitor cocktail (Cwbiotech, Beijing, China). .. Protein lysates were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blotting assays were performed using the antibodies specific to NRDE2 (1:1,000; 24968-1-AP, Proteintech, China), ATM (1:1,000; 2873T, Cell Signaling Technology, USA), ATR (1:1,000; 2790S, Cell Signaling Technology, USA), CHK1 (1:1,000; 2360S, Cell Signaling Technology, USA), CHK2 (1:1,000; 3440S, Cell Signaling Technology, USA), CK2A1 (1:2,000; 10992-1-AP, Proteintech, China), CK2A2 (1:2,000; 10606-1-AP, Proteintech, China), CK2B (1:2,000; NBP1-06514, Nouvs Biologicals, USA), CK2 phosphorylation (1:1,000; 8738, Cell Signaling Technology, USA), Flag (1:2,000; F3165, Sigma, USA), HA (1:2,000; M180-3, MBL, Japan), Histone H3 (1:1,000; 17168-1-AP, Proteintech, China), Western Blot:Article Title: NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors Article Snippet: Cells were harvested in phosphate buffer saline (PBS) (4°C), and cell pellets were lysed with NETN buffer containing complete protease inhibitor cocktail (Roche, Germany) and phosphatase inhibitor cocktail (Cwbiotech, Beijing, China). .. Protein lysates were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blotting assays were performed using the antibodies specific to NRDE2 (1:1,000; 24968-1-AP, Proteintech, China), ATM (1:1,000; 2873T, Cell Signaling Technology, USA), ATR (1:1,000; 2790S, Cell Signaling Technology, USA), CHK1 (1:1,000; 2360S, Cell Signaling Technology, USA), CHK2 (1:1,000; 3440S, Cell Signaling Technology, USA), CK2A1 (1:2,000; 10992-1-AP, Proteintech, China), CK2A2 (1:2,000; 10606-1-AP, Proteintech, China), CK2B (1:2,000; NBP1-06514, Nouvs Biologicals, USA), CK2 phosphorylation (1:1,000; 8738, Cell Signaling Technology, USA), Flag (1:2,000; F3165, Sigma, USA), HA (1:2,000; M180-3, MBL, Japan), Histone H3 (1:1,000; 17168-1-AP, Proteintech, China), Article Title: NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors. Article Snippet: Immunoblotting assays Cells were harvested in phosphate buffer saline (PBS) (4 C), and cell pellets were lysed with NETN buffer containing complete protease inhibitor cocktail (Roche, Germany) and phosphatase inhibitor cocktail (Cwbiotech, Beijing, China). .. Protein lysates were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blotting assays were performed using the antibodies specific to NRDE2 (1:1,000; 24968-1-AP, Proteintech, China), ATM (1:1,000; 2873T, Cell Signaling Technology, USA), ATR (1:1,000; 2790S, Cell Signaling Technology, USA), CHK1 (1:1,000; 2360S, Cell Signaling Technology, USA), CHK2 (1:1,000; 3440S, Cell Signaling Technology, USA), CK2A1 (1:2,000; 10992-1-AP, Proteintech, China), CK2A2 (1:2,000; 10606-1-AP, Proteintech, China), CK2B (1:2,000; NBP1-06514, Nouvs Biologicals, USA), CK2 phosphorylation (1:1,000; 8738, Cell Signaling Technology, USA), Flag (1:2,000; F3165, Sigma, USA), HA (1:2,000; M180-3, MBL, Japan), Histone H3 (1:1,000; 17168-1-AP, Proteintech, China), Phospho-proteomics:Article Title: NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors Article Snippet: Cells were harvested in phosphate buffer saline (PBS) (4°C), and cell pellets were lysed with NETN buffer containing complete protease inhibitor cocktail (Roche, Germany) and phosphatase inhibitor cocktail (Cwbiotech, Beijing, China). .. Protein lysates were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blotting assays were performed using the antibodies specific to NRDE2 (1:1,000; 24968-1-AP, Proteintech, China), ATM (1:1,000; 2873T, Cell Signaling Technology, USA), ATR (1:1,000; 2790S, Cell Signaling Technology, USA), CHK1 (1:1,000; 2360S, Cell Signaling Technology, USA), CHK2 (1:1,000; 3440S, Cell Signaling Technology, USA), CK2A1 (1:2,000; 10992-1-AP, Proteintech, China), CK2A2 (1:2,000; 10606-1-AP, Proteintech, China), CK2B (1:2,000; NBP1-06514, Nouvs Biologicals, USA), CK2 phosphorylation (1:1,000; 8738, Cell Signaling Technology, USA), Flag (1:2,000; F3165, Sigma, USA), HA (1:2,000; M180-3, MBL, Japan), Histone H3 (1:1,000; 17168-1-AP, Proteintech, China), Article Title: NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors. Article Snippet: Immunoblotting assays Cells were harvested in phosphate buffer saline (PBS) (4 C), and cell pellets were lysed with NETN buffer containing complete protease inhibitor cocktail (Roche, Germany) and phosphatase inhibitor cocktail (Cwbiotech, Beijing, China). .. Protein lysates were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blotting assays were performed using the antibodies specific to NRDE2 (1:1,000; 24968-1-AP, Proteintech, China), ATM (1:1,000; 2873T, Cell Signaling Technology, USA), ATR (1:1,000; 2790S, Cell Signaling Technology, USA), CHK1 (1:1,000; 2360S, Cell Signaling Technology, USA), CHK2 (1:1,000; 3440S, Cell Signaling Technology, USA), CK2A1 (1:2,000; 10992-1-AP, Proteintech, China), CK2A2 (1:2,000; 10606-1-AP, Proteintech, China), CK2B (1:2,000; NBP1-06514, Nouvs Biologicals, USA), CK2 phosphorylation (1:1,000; 8738, Cell Signaling Technology, USA), Flag (1:2,000; F3165, Sigma, USA), HA (1:2,000; M180-3, MBL, Japan), Histone H3 (1:1,000; 17168-1-AP, Proteintech, China), Incubation:Article Title: SFN Enhanced the Radiosensitivity of Cervical Cancer Cells via Activating LATS2 and Blocking Rad51/MDC1 Recruitment to DNA Damage Site Article Snippet: .. Cells were grown on coverslips and were fixed with 4% paraformaldehyde for 20 min, blocked with mixture of 5% bovine serum albumin and 0.2% Triton X-100 for 30 min, and incubated with antibodies against LATS2 (1:100, Proteintech, Wuhan, China), γ-H2AX (1:400, Cell Signaling Technology), Rad51 (1:300, Abcam), and |