mcph1 (Proteintech)
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Proteintech
mcph1
Mcph1, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcph1/MCPH%2FBRIT1+Antibody/pm41931484-79-32-34
Average 91 stars, based on 10 article reviews
Mcph1, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcph1/MCPH%2FBRIT1+Antibody/pm41931484-79-32-34
Average 91 stars, based on 10 article reviews
mcph1 - by Bioz Stars,
2026-10
91/100 stars
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Related Articles
Incubation:Article Title: Differential sensitivity of MCPH1- and BRCA2-deficient cancer cells to PARP-1 inhibition. Article Snippet: Protein electrophoresis was carried out using 3−8% Tris-Acetate or 4−12% bis tris NU-PAGE gels (Invitrogen) prior to transfer to nitrocellulose membrane using transfer stacks and an iBlot2 machine (both Invitrogen). .. Membranes were blocked with 5% dried skimmed milk/TBS-T (50mM Tris pH 7.6, 150mM NaCl and 0.2% v/v Tween-20) for a minimum of 1-hour prior to overnight incubation at 4°C with primary antibodies; Article Title: Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1 Article Snippet: Protein electrophoresis was performed by using either 3–8% Tris acetate or 4–12% bis Tris gels (Thermo Fisher Scientific) before transferring to nitrocellulose by using an iBlot2 machine. .. Membranes were blocked in either 5% dried skimmed milk/TBST or 2.5% bovine serum albumin/TBST (depending on the primary antibody used) for 1 h before overnight incubation in blocking buffer containing the following primary antibodies: Article Title: Phosphorylation of MCPH1 isoforms during mitosis followed by isoform‐specific degradation by APC/C‐CDH1 Article Snippet: Protein electrophoresis was performed by using either 3–8%Tris acetate or 4–12% bis Tris gels (Thermo Fisher Scientific) before transferring to nitrocellulose by using an iBlot2 machine. .. Membranes were blocked in either 5% dried skimmed milk/TBST or 2.5% bovine serum albumin/TBST (depending on the primary antibody used) for 1 h before overnight incubation in blocking buffer containing the following primary antibodies: Article Title: Differential sensitivity of MCPH1- and BRCA2-deficient cancer cells to PARP-1 inhibition Article Snippet: Protein electrophoresis was carried out using 3−8% Tris-Acetate or 4−12% bis tris NU-PAGE gels (Invitrogen) prior to transfer to nitrocellulose membrane using transfer stacks and an iBlot2 machine (both Invitrogen). .. Membranes were blocked with 5% dried skimmed milk/TBS-T (50mM Tris pH 7.6, 150mM NaCl and 0.2% v/v Tween-20) for a minimum of 1-hour prior to overnight incubation at 4°C with primary antibodies; Transfection:Article Title: Phosphorylation of MCPH1 isoforms during mitosis followed by isoform‐specific degradation by APC/C‐CDH1 Article Snippet: After incubation with the relevant horseradish peroxidase–conjugated secondary antibody, Western blots were visualized with ECL Prime (GEHealthcare,Chicago, IL,USA) and images capturedby using a Syngene (Cambridge, United Kingdom) G:Box gel documentation system. .. HEK293 cells were transfected with GFP–MCPH1 for 24 h using calcium phosphate before treatment with nocodazole for 20 h. Mitotic cells were isolated by using the shake-off procedure, lysed in NETN buffer (as previously described), and GFP– Isolation:Article Title: Phosphorylation of MCPH1 isoforms during mitosis followed by isoform‐specific degradation by APC/C‐CDH1 Article Snippet: After incubation with the relevant horseradish peroxidase–conjugated secondary antibody, Western blots were visualized with ECL Prime (GEHealthcare,Chicago, IL,USA) and images capturedby using a Syngene (Cambridge, United Kingdom) G:Box gel documentation system. .. HEK293 cells were transfected with GFP–MCPH1 for 24 h using calcium phosphate before treatment with nocodazole for 20 h. Mitotic cells were isolated by using the shake-off procedure, lysed in NETN buffer (as previously described), and GFP– Blocking Assay:Article Title: Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1 Article Snippet: Protein electrophoresis was performed by using either 3–8% Tris acetate or 4–12% bis Tris gels (Thermo Fisher Scientific) before transferring to nitrocellulose by using an iBlot2 machine. .. Membranes were blocked in either 5% dried skimmed milk/TBST or 2.5% bovine serum albumin/TBST (depending on the primary antibody used) for 1 h before overnight incubation in blocking buffer containing the following primary antibodies: Article Title: Phosphorylation of MCPH1 isoforms during mitosis followed by isoform‐specific degradation by APC/C‐CDH1 Article Snippet: Protein electrophoresis was performed by using either 3–8%Tris acetate or 4–12% bis Tris gels (Thermo Fisher Scientific) before transferring to nitrocellulose by using an iBlot2 machine. .. Membranes were blocked in either 5% dried skimmed milk/TBST or 2.5% bovine serum albumin/TBST (depending on the primary antibody used) for 1 h before overnight incubation in blocking buffer containing the following primary antibodies: |