mbp  (Bio-Rad)

Journal: bioRxiv

Article Title: Red fluorescent labeling of myelin by membrane-targeted tdTomato in transgenic mouse lines

doi: 10.64898/2026.04.17.718425

Figure Lengend Snippet: Upper panels: Fluorescence micrographs visualizing that transgenically expressed tdTomato is localized in the processes and somata of oligodendrocytes which are marked by CNP immunolabeling. A fraction of CNP-positive oligodendrocytes does not express tdTomato (purple arrows), corresponding to the expression pattern as described in . Lower panels: Colocalization of MBP and tdTomato in myelin. The fluorescence signal is observed on both sides of the axon, while the enwrapped axon shows no fluorescence (white arrow in the inset). The cyan arrows highlight primary processes of oligodendrocytes connecting soma and myelin sheaths, which are labeled by tdTomato, but not stained for MBP. CB: cerebellum; CTX: cortex; HIP: hippocampus. The letters in the circles indicate the CNPMTO mouse line. Scale bar: 10 µm. Scale bar inset: 4 µm.

Article Snippet: Free-floating slices were permeabilized in 0.4% Triton X-100/PBS for 30 min and blocked with 4% fetal calf serum (FCS) in 0.2% Triton X-100/PBS for 1 h. Sections were immunohistochemically stained either for MBP (rat anti-MBP, AbD Serotec #MCA409S) or CNP (guinea pig anti-CNP, Synaptic Systems #355004).

Techniques: Fluorescence, Immunolabeling, Expressing, Labeling, Staining

Journal: bioRxiv

Article Title: Synaptic Alterations Are Preceding the Axonal Loss in Optic Atrophy of Wolfram Syndrome Mouse Model

doi: 10.64898/2026.03.22.713521

Figure Lengend Snippet: (A) Optic nerve sections were immunostained with an anti-MBP antibody (green). Representative confocal images from 7-month-old WT (top) and Wfs1 KO (bottom) mice are shown along with the corresponding high-magnification zoom of the boxed area. Scale bar, 50 µm. Quantification of MBP fluorescence mean intensity in optic nerve sections from (B) 4-month (C) 7-month old animals. Data are presented as normalized values ± SEM relative to the WT mean. Statistical analysis was performed using a two-tailed unpaired Student’s I-test with Welch’s correction. n≥ 5. ns - not significant.

Article Snippet: Primary antibodies against Brn3a (MAB1585, Millipore, 1:250), RBPMS (GTX118619, GeneTex, 1:200), NF200 (N4142, Sigma-Aldrich, 1:2000), GFAP (PA1-10004, Invitrogen, 1:2000), MBP aa82–87 (MCA409S, Bio-Rad, 1:50), β-III Tubulin (ab18207, Abcam, 1:750), PSD95 (51-6900, Invitrogen, 1:150), and Synaptophysin (101 004, Synaptic Systems, 1:2000) were applied overnight at 4°C.

Techniques: Fluorescence, Two Tailed Test