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mca2308  (Bio-Rad)


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    Structured Review

    Bio-Rad mca2308
    Susceptibility to ApxIIIA-induced cell death closely depends on the rate of LFA-1 ectopic expression in human erythroleukemic K-562 cells provided the contributing CD18 subunit is that of the pig. Values (squares, circles, triangles and lozenges) are means ± SD from three representative experiments in which mean LFA-1 expression rate (horizontal SD) and mean PI incorporation (vertical SD) were measured by flow cytometry. LFA-1 cell-surface expression rate typical of each experiment was defined as the mean proportion of Alexa 488-positive cells calculated from two independent labelling procedures, one using MCA1972 (anti-CD18) and the other <t>MCA2308</t> or 555382 <t>(anti-CD11a)</t> as primary antibody.
    Mca2308, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mca2308/Mouse+anti+Pig+CD11a/pmc02701182-25-5-11
    Average 93 stars, based on 15 article reviews
    mca2308 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity"

    Article Title: Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity

    Journal: Veterinary Research

    doi: 10.1051/vetres/2009016

    Susceptibility to ApxIIIA-induced cell death closely depends on the rate of LFA-1 ectopic expression in human erythroleukemic K-562 cells provided the contributing CD18 subunit is that of the pig. Values (squares, circles, triangles and lozenges) are means ± SD from three representative experiments in which mean LFA-1 expression rate (horizontal SD) and mean PI incorporation (vertical SD) were measured by flow cytometry. LFA-1 cell-surface expression rate typical of each experiment was defined as the mean proportion of Alexa 488-positive cells calculated from two independent labelling procedures, one using MCA1972 (anti-CD18) and the other MCA2308 or 555382 (anti-CD11a) as primary antibody.
    Figure Legend Snippet: Susceptibility to ApxIIIA-induced cell death closely depends on the rate of LFA-1 ectopic expression in human erythroleukemic K-562 cells provided the contributing CD18 subunit is that of the pig. Values (squares, circles, triangles and lozenges) are means ± SD from three representative experiments in which mean LFA-1 expression rate (horizontal SD) and mean PI incorporation (vertical SD) were measured by flow cytometry. LFA-1 cell-surface expression rate typical of each experiment was defined as the mean proportion of Alexa 488-positive cells calculated from two independent labelling procedures, one using MCA1972 (anti-CD18) and the other MCA2308 or 555382 (anti-CD11a) as primary antibody.

    Techniques Used: Expressing, Flow Cytometry

    Related Articles

    Expressing:

    Article Title: Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity
    Article Snippet: The K-562 cell line was obtained from American Type Culture Collection (#CCL-243) and was maintained in DMEM medium at 37 °C in a humidified atmosphere of 5% CO 2 .The K-562 cell line was obtained from American Type Culture Collection (#CCL-243) and was maintained in DMEM medium at 37 °C in a humidified atmosphere of 5% CO 2 .. MAbs MCA1972 (anti-pig CD18) and MCA2308 (anti-pig CD11a) were purchased from Abd Serotec (Düsseldorf, Germany), mAb BAQ30A (anti-bovine CD18) from VMRD (Pullman, USA), mAb 555382 (anti-human/bovine CD11a) from BD Biosciences (Erembodegem, Belgium) and AlexaFluor ® 488-conjugated goat anti-mouse IgG from Invitrogen (Carlsbad, CA, USA).

    Flow Cytometry:

    Article Title: Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity
    Article Snippet: The K-562 cell line was obtained from American Type Culture Collection (#CCL-243) and was maintained in DMEM medium at 37 °C in a humidified atmosphere of 5% CO 2 .The K-562 cell line was obtained from American Type Culture Collection (#CCL-243) and was maintained in DMEM medium at 37 °C in a humidified atmosphere of 5% CO 2 .. MAbs MCA1972 (anti-pig CD18) and MCA2308 (anti-pig CD11a) were purchased from Abd Serotec (Düsseldorf, Germany), mAb BAQ30A (anti-bovine CD18) from VMRD (Pullman, USA), mAb 555382 (anti-human/bovine CD11a) from BD Biosciences (Erembodegem, Belgium) and AlexaFluor ® 488-conjugated goat anti-mouse IgG from Invitrogen (Carlsbad, CA, USA).



    Similar Products

    mca2308  (Bio-Rad)
    93
    Bio-Rad mca2308
    Susceptibility to ApxIIIA-induced cell death closely depends on the rate of LFA-1 ectopic expression in human erythroleukemic K-562 cells provided the contributing CD18 subunit is that of the pig. Values (squares, circles, triangles and lozenges) are means ± SD from three representative experiments in which mean LFA-1 expression rate (horizontal SD) and mean PI incorporation (vertical SD) were measured by flow cytometry. LFA-1 cell-surface expression rate typical of each experiment was defined as the mean proportion of Alexa 488-positive cells calculated from two independent labelling procedures, one using MCA1972 (anti-CD18) and the other <t>MCA2308</t> or 555382 <t>(anti-CD11a)</t> as primary antibody.
    Mca2308, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mca2308/Mouse+anti+Pig+CD11a/pmc02701182-25-5-11
    Average 93 stars, based on 1 article reviews
    mca2308 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Susceptibility to ApxIIIA-induced cell death closely depends on the rate of LFA-1 ectopic expression in human erythroleukemic K-562 cells provided the contributing CD18 subunit is that of the pig. Values (squares, circles, triangles and lozenges) are means ± SD from three representative experiments in which mean LFA-1 expression rate (horizontal SD) and mean PI incorporation (vertical SD) were measured by flow cytometry. LFA-1 cell-surface expression rate typical of each experiment was defined as the mean proportion of Alexa 488-positive cells calculated from two independent labelling procedures, one using MCA1972 (anti-CD18) and the other MCA2308 or 555382 (anti-CD11a) as primary antibody.

    Journal: Veterinary Research

    Article Title: Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity

    doi: 10.1051/vetres/2009016

    Figure Lengend Snippet: Susceptibility to ApxIIIA-induced cell death closely depends on the rate of LFA-1 ectopic expression in human erythroleukemic K-562 cells provided the contributing CD18 subunit is that of the pig. Values (squares, circles, triangles and lozenges) are means ± SD from three representative experiments in which mean LFA-1 expression rate (horizontal SD) and mean PI incorporation (vertical SD) were measured by flow cytometry. LFA-1 cell-surface expression rate typical of each experiment was defined as the mean proportion of Alexa 488-positive cells calculated from two independent labelling procedures, one using MCA1972 (anti-CD18) and the other MCA2308 or 555382 (anti-CD11a) as primary antibody.

    Article Snippet: MAbs MCA1972 (anti-pig CD18) and MCA2308 (anti-pig CD11a) were purchased from Abd Serotec (Düsseldorf, Germany), mAb BAQ30A (anti-bovine CD18) from VMRD (Pullman, USA), mAb 555382 (anti-human/bovine CD11a) from BD Biosciences (Erembodegem, Belgium) and AlexaFluor ® 488-conjugated goat anti-mouse IgG from Invitrogen (Carlsbad, CA, USA).

    Techniques: Expressing, Flow Cytometry