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Bio-Rad blocking solution
Blocking Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mca1853/Mouse+anti+Human+CD163/pm41937075-87-39-36
Average 94 stars, based on 237 article reviews

blocking solution - by Bioz Stars, 2026-09

94/100 stars

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Article Title: Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus
Article Snippet: CD163 , 1/200 , Mouse MAb , Bio-Rad, MCA1853.

Formalin-fixed Paraffin-Embedded:

Article Title: Spatial analysis identifies DC niches as predictors of pembrolizumab therapy in head and neck squamous cell cancer
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Pan Cytokeratin Biolegend Cat# 914204; RRID: AB_2616960 Anti-P63 Abcam Cat# ab735; RRID: AB_305870 Anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Anti-EGFR R&D Cat# AF231; RRID: AB_355220 Anti-EPCAM R&D Cat# AF960; RRID: AB_355745 Anti-Her2 Bio-Rad Cat# MCA1788; RRID: AB_322791 Anti-MUC1 Biolegend Cat# 355602; RRID: AB_2561642 Anti-CD45 Cell Signaling Technology Cat# 47937; RRID: AB_2922773 Anti-CD3 Abcam Cat# ab11089; RRID: AB_2889189 Anti-CD4 Abcam Cat# ab280849; RRID: AB_3107080 Anti-CD8 Cell Signaling Technology Cat# 85336; RRID: AB_2800052 Anti-FoxP3 Thermofisher Cat# 14-4777-80; RRID: AB_467555 Anti-CD20 Thermofisher Cat# 14-0202-82; RRID: AB_10734340 Anti-CD11b Cell Signaling Technology Cat# 23743; RRID: AB_3683724 Anti-CD68 Biolegend Cat# 916104; RRID: AB_2616797 Anti-CD14 Cell Signaling Technology Cat# 43878; RRID: AB_3683725 Anti-CD11c Cell Signaling Technology Cat# 45581SF; RRID: AB_2799286 Anti-LAMP3 Atlas Antibodies Cat# HPA051467; RRID: AB_2681475 Anti-CD66b Biolegend Cat# 305102; RRID: AB_314494 Anti-PD-L1 Cell Signaling Technology Cat# 13684; RRID: AB_2687655 Anti-PD-1 Cell Signaling Technology Cat# 63815SF; RRID: AB_3675993 Anti-CTLA4 Abcam Cat# ab237712; RRID: AB_2905652 Anti-TCF1 R&D Cat# AF5596; RRID: AB_2199293 Anti-CD163 Bio-Rad Cat# MCA1853; RRID: AB_2074540 Anti-Rabbit IgG Thermofisher Cat# 31210; RRID: AB_228334 Anti-Mouse IgG Biolegend Cat# 405301; RRID: AB_315005 Anti-Rat IgG Abcam Cat# ab97053; RRID: AB_10698225 Biological samples HNSCC FFPE sections Participants of this study N/A Chemicals, peptides, and recombinant proteins AFDye 488 TFP Ester Fluoroprobes Cat# 1026-5 AFDye 555 NHS Ester Fluoroprobes Cat# 1166-5 AFDye 647 NHS Ester Click Chemistry Tools Cat# 1121-5 dTCO-PNP Dr. Hannes Mikula https://www.ias.tuwien.ac.at/hannesmikula BHQ®-3 Amine LGC Biosearch Technologies Cat# BHQ-3001-5 Amino-dPEG4-carboxylic acids Quanta BioDesign Cat# 10244 Amino-dPEG6-carboxylic acids Quanta BioDesign Cat# 10067 N-⍺-Boc-N-ε-Fmoc-Lysine Chem-Impex Cat# 00037 Dimethyl Sulfoxide Millipore Sigma Cat# 472301-500ML Software and algorithms GraphPad Prism (v.10.1.1) GraphPad software RRID: SCR_002798 FIJI ImageJ (v.2.1.0) FIJI https://fiji.sc; RRID: SCR_002285 (Continued on next page) Cell Reports Medicine 6, 102100, May 20, 2025 e1 Article ll OPEN ACCESS ..

Article Title: Spatial analysis identifies DC niches as predictors of pembrolizumab therapy in head and neck squamous cell cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Pan Cytokeratin Biolegend Cat# 914204; RRID: AB_2616960 Anti-P63 Abcam Cat# ab735; RRID: AB_305870 Anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Anti-EGFR R&D Cat# AF231; RRID: AB_355220 Anti-EPCAM R&D Cat# AF960; RRID: AB_355745 Anti-Her2 Bio-Rad Cat# MCA1788; RRID: AB_322791 Anti-MUC1 Biolegend Cat# 355602; RRID: AB_2561642 Anti-CD45 Cell Signaling Technology Cat# 47937; RRID: AB_2922773 Anti-CD3 Abcam Cat# ab11089; RRID: AB_2889189 Anti-CD4 Abcam Cat# ab280849; RRID: AB_3107080 Anti-CD8 Cell Signaling Technology Cat# 85336; RRID: AB_2800052 Anti-FoxP3 Thermofisher Cat# 14-4777-80; RRID: AB_467555 Anti-CD20 Thermofisher Cat# 14-0202-82; RRID: AB_10734340 Anti-CD11b Cell Signaling Technology Cat# 23743; RRID: AB_3683724 Anti-CD68 Biolegend Cat# 916104; RRID: AB_2616797 Anti-CD14 Cell Signaling Technology Cat# 43878; RRID: AB_3683725 Anti-CD11c Cell Signaling Technology Cat# 45581SF; RRID: AB_2799286 Anti-LAMP3 Atlas Antibodies Cat# HPA051467; RRID: AB_2681475 Anti-CD66b Biolegend Cat# 305102; RRID: AB_314494 Anti-PD-L1 Cell Signaling Technology Cat# 13684; RRID: AB_2687655 Anti-PD-1 Cell Signaling Technology Cat# 63815SF; RRID: AB_3675993 Anti-CTLA4 Abcam Cat# ab237712; RRID: AB_2905652 Anti-TCF1 R&D Cat# AF5596; RRID: AB_2199293 Anti-CD163 Bio-Rad Cat# MCA1853; RRID: AB_2074540 Anti-Rabbit IgG Thermofisher Cat# 31210; RRID: AB_228334 Anti-Mouse IgG Biolegend Cat# 405301; RRID: AB_315005 Anti-Rat IgG Abcam Cat# ab97053; RRID: AB_10698225 Biological samples HNSCC FFPE sections Participants of this study N/A Chemicals, peptides, and recombinant proteins AFDye 488 TFP Ester Fluoroprobes Cat# 1026-5 AFDye 555 NHS Ester Fluoroprobes Cat# 1166-5 AFDye 647 NHS Ester Click Chemistry Tools Cat# 1121-5 dTCO-PNP Dr. Hannes Mikula https://www.ias.tuwien.ac.at/hannesmikula BHQ®-3 Amine LGC Biosearch Technologies Cat# BHQ-3001-5 Amino-dPEG4-carboxylic acids Quanta BioDesign Cat# 10244 Amino-dPEG6-carboxylic acids Quanta BioDesign Cat# 10067 N-⍺-Boc-N-ε-Fmoc-Lysine Chem-Impex Cat# 00037 Dimethyl Sulfoxide Millipore Sigma Cat# 472301-500ML Software and algorithms GraphPad Prism (v.10.1.1) GraphPad software RRID: SCR_002798 FIJI ImageJ (v.2.1.0) FIJI https://fiji.sc; RRID: SCR_002285 (Continued on next page) Cell Reports Medicine 6, 102100, May 20, 2025 e1 Article ll OPEN ACCESS ..

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Article Title: Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6.
Article Snippet: The scavenger receptor CD163 is widely used as a cell signature for alternatively active “M2” macrophages in mammals.. In this study, we generated two monoclonal antibodies, ABM-1A9 and ABM-2D6, against the extracellular region of bovine CD163.. Conventional Western blotting using the antibodies yielded immunoreactive bands of bovine CD163 at 130 and 150 kDa in non-reduced and reduced spleen lysates, respectively.

Article Title: CD14 + monocytes and CD163 + macrophages correlate with the severity of liver fibrosis in patients with chronic hepatitis C
Article Snippet: The plasma HCV RNA load was measured by using qualitative reverse transcription PCR (RT-PCR) assay (Cobas Taqman HCV Test; Roche Diagnostics) and the lower limit of quantification was 15 IU/ml. .. Paraffin-embedded liver sections (5 μm) were incubated with anti-CD163 (specific for M2 macrophages) (1:100 dilution; cat. no. MCA1853; AbD Serotec) and EnVision System HRP-conjugated secondary antibody (cat. no. K4001; Dako; Agilent Technologies, Inc.). ..

Immunopeptidomics:

Article Title: Spatially resolved single-cell atlas unveils a distinct cellular signature of fatal lung COVID-19 in a Malawian population
Article Snippet: .. rabbit anti-SARS-CoV-2 nucleocapsid protein (5ug/ml) Novus Biologicals Cat# NB100-56576 rabbit anti-human CD31 (2ug/ml) Novus Biologicals Cat# NB100-2284 rabbit anti-human SP-C (4.17ug/ml) Novus Biologicals Cat# NBP1-60117 mouse anti-human CD68 (5ug/ml) Agilent Cat# M0876 rabbit anti-human KRT8 (2.5ug/ml) Abcam Cat# ab59400 anti-human Smooth muscle actin (SMA) (clone 1A4) Bio-Rad Cat# MCA5781GA anti-humanCD68 (clone KP1) Thermo Cat# MA5-13324 anti-human CD235ab (clone HIR2) BioLegend Cat# 306615 anti-human Pan-cytokeratin (PanCK) (clone AE-1/AE-3) Biolegend Cat# 914204 anti-human CD38 (clone EPR4106) Standard Biotools Cat# 3141018D anti-human MHC I (clone EMR8-5) Abcam Cat# ab70328 anti-human Vimentin (clone RV202) Standard Biotools Cat# 3143029D anti-human CD14 (clone D7A2T) Cell Signalling Cat# 56082BF anti-human ICAM1 (clone EP1442Y) Abcam Cat# ab271852 anti-human CD16 (clone SP175) Abcam Cat# ab243925100ug anti-human iNOS (clone SP126) abcam Cat# ab239990 anti-human CD66b (G10F5) Novus Cat# g10f5_nb10077808 anti-human CD11b (EP1345Y) Abcam Cat# ab5247 anti-human CD44 (IM7) BioLegend Cat# 103001 anti-human CD107a (H4A3) Standard Biotools Cat# 3151021D anti-human CD45 (CD45-2B11) eBioscience Cat# 14-9457-82 anti-human CD31 (JC/70A) Novus Cat# jc-70a_nb600562 anti-human CD11c (Polyclonal) Standard Biotools Cat# 3154025D anti-human Foxp3 (clone 236A/E7) Abcam Cat# ab20034 anti-human CD4 (clone EPR6855) Standard Biotools Cat# 3156033D anti-human SARS-Cov-2 (Polyclonal) Novus Cat# NB100-56576 anti-human Von Willebrand Factor (vWF) (Polyclonal) Dako Cat# A0082 anti-human Vista (clone D1L2G) Standard Biotools Cat# 3160025D anti-human CD20 (clone H1) Standard Biotools Cat# 3161029D anti-human CD8 (clone CD8/144B) eBioscience Cat# 14-0085-82 anti-human Iba1 (Polyclonal) WAKO Cat# 019-19741 anti-human Arginase 1 (clone D4E3M) Standard Biotools Cat# 3164027D anti-human Fibrinogen (EPR18145-84) Abcam Cat# ab227063 anti-human CD74 (clone LN2) Standard Biotools Cat# 3166025D anti-human Granzyme B (clone EPR20129-217) Standard Biotools Cat# 3167021D anti-human Collagen Type I (Polyclonal) Standard Biotools Cat# 3169023D anti-human CD3 (clone D7A6E) Cell Signalling Cat# 85061BF anti-human pERK1/2 [T202/Y204] (clone D13.14.4E) Standard Biotools Cat# 3171021D anti-human Cleaved Caspase 3 (clone 5A1E) Standard Biotools Cat# 3172023A anti-human CD45RO (clone UCHL1) Standard Biotools Cat# 3173016D anti-human MHC II (clone TAL1B5) Abcam Cat# ab176408 anti-human CD206 (clone E2L9N) Cell Signalling Cat# 91992 anti-human CD163 (clone EDHu-1) Bio-Rad Cat# MCA1853 .. Package name Reference (where applicable) URL R v4.3.0 https://www.cran.r-project.org Python v3.9.6 https://www.python.org RStudio software (v 2023.03.0+386; 2023) The R project for Statistical Computing https://www.rstudio.com/ and https://cran.r-project.org/ Ilastik v1.4.0 Berg et al., 2019 https://www.ilastik.org CellProfiler v4.2.4 https://cellprofiler.org FactoMineR v2.8 (R package) Sebastien Le, 2008 https://cran.r- project.org/web/packages/Fact oMineR/index.html ggplot2 v3.4.2 (R package) https://ggplot2.tidyverse.org factoextra v1.0.7 (R package) https://cran.r- project.org/web/packages/facto extra/index.html corrplot v0.92 (R package) https://cran.r- project.org/web/packages/corr plot/vignettes/corrplot- intro.html missMDA v1.18 (R package) Josse and Husson, 2016 https://cran.r- project.org/web/packages/miss MDA/index.html tidymodels v1.0.0 (R package) https://www.tidymodels.org cytomapper v1.9.2 (R package) Nils Eling, 2020 https://github.com/Bodenmiller Group/cytomapper miloR v1.4.0 (R package) (Dann et al., 2022) https://marionilab.github.io/mil oR Giotto v3.3.0 (R package) Dries et al., 2021 https://giottosuite.readthedocs. io/en/latest/ ImcSegmentationPipeline (Python package) Vito RT Zanotelli, 2022 https://github.com/Bodenmiller Group/ImcSegmentationPipelin e IMC-Denoise (Python package) Lu et al., 2023 https://github.com/PENGLU- WashU/IMC_Denoise DeepCell (Python package) Greenwald et al., 2022 https://github.com/vanvalenlab /deepcell-tf Scanpy (Single-Cell Analysis in Python) v1.9.1 (Python package) (Wolf et al., 2018 https://github.com/scverse/sca npy umap-learn v0.5.3 (Python package) (McInnes, 2018 https://github.com/lmcinnes/u map Astir (ASsignmenT of sIngle-cell pRoteomics) v0.1.4 (Python package) Geuenich et al., 2021 https://github.com/camlab- bioml/astir Squidpy (Spatial Quantification of Molecular Data in Python) v1.2.2 (Python package) Palla et al., 2022 https://squidpy.readthedocs.io/ en/stable/ Athena (Analysis of Tumor HEterogeNeity from spAtial omics measurements) v 0.1.3 (Python package) (Martinelli and Rapsomaniki, 2022 https://ai4scr.github.io/ATHENA /index.html SpOOx (Spatial Omics Oxford Pipeline) (Python package) (Praveen Weeratunga, 2022 https://github.com/Taylor-CCB- Group/SpOOx Supplemental Figure 1 | Histological characteristics of lung pathology in patients with SARS CoV-2 infection A B C D E F * * * * * * * * * * * * ** * * * * Supplemental data, Fig. 1 Supplemental Figure 2 | Top cluster markers characterising immune and stromal cell populations in the lung in the Malawi cohort Supplemental Figure 3 | Characterisation of cell populations in the nasal and peripheral blood tissue compartments in the Malawi cohort.

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