math1 (Proteintech)
Structured Review

Math1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/math1/ATOH1+Antibody/pmc11794537-69-43-45
Average 94 stars, based on 63 article reviews
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1) Product Images from "Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis"
Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis
Journal: Frontiers in Pharmacology
doi: 10.3389/fphar.2024.1534772
Figure Legend Snippet: PAE inhibits the Notch1/Math1 pathway in UC mice. (A) Western blot images of Notch1 and Math1 in the colon. (B, C) Analysis of gray-scale of Notch1 and Math1 protein expression. (D) Immunohistochemistry images of Notch1 in colon (×100). (E) Immunohistochemistry images of Math1 in the colon (×100). (n = 3–6, * P < 0.05 and ** P < 0.01 versus control group, # P < 0.05 and ## P < 0.01 versus model group).
Techniques Used: Western Blot, Expressing, Immunohistochemistry, Control
Figure Legend Snippet: PAE attenuates the disruption of tight junction proteins in HCoEpic cells by regulating activated Notch1-mediated Th17/Treg imbalance in vitro . (A) Effect of VPA on the viability of Jurkat T cells analyzed with CCK8 assay. (B) Effect of PAE on the viability of Jurkat cells analyzed with CCK-8 assay. (C) Western blot images of Notch1, Math1, RORγt, and Foxp3 in Jurkat T cells. (D–G) Analysis of gray-scale of Notch1, Math1, RORγt, and Foxp3 protein expression. (H) After notch1-activated Jurkat T cells were co-cultured with HCoEpic cells, Western blot images of occluding, claudin1, and ZO1 in HCoEpic cells. (I–K) Analysis of gray-scale of occluding, claudin1, and ZO1 protein expression. (n = 5–8, * P < 0.05 and ** P < 0.01 versus control group, # P < 0.05 and ## P < 0.01 versus VPA group).
Techniques Used: Disruption, In Vitro, CCK-8 Assay, Western Blot, Expressing, Cell Culture, Control
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Blocking Assay:Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis Article Snippet: The equal amount of protein was separated with sodium dodecyl sulfate -polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore, LG18-1501M-1H). .. After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1-AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis. Article Snippet: The equal amount of protein was separated with sodium dodecyl sulfate -polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore, LG181501M-1H). .. After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1- AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Incubation:Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis Article Snippet: The equal amount of protein was separated with sodium dodecyl sulfate -polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore, LG18-1501M-1H). .. After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1-AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis. Article Snippet: The equal amount of protein was separated with sodium dodecyl sulfate -polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore, LG181501M-1H). .. After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1- AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), |