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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies
doi: 10.1016/j.xpro.2026.104455
Figure Lengend Snippet: Stepwise overview of the transduction procedure Depending on viral titer, microplate format and number of wells to be transduced AAV can be pre-diluted or added straight to freshly made medium containing doxorubicin for subsequent overnight incubation.
Article Snippet:
Techniques: Transduction, Incubation
Journal: STAR Protocols
Article Title: Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies
doi: 10.1016/j.xpro.2026.104455
Figure Lengend Snippet: Overview of AAV titration experiment and expected results AAV particles were diluted to final concentration in cell medium containing 300 nM doxorubicin and applied (using a full medium replacement) to hiMG at indicated concentrations (vg/mL). Fluorescence imaging of mScarlet expression was performed in live cultures 5 days post transduction. Optimal concentration was found to be around 6–8 × 10 8 vg/mL.
Article Snippet:
Techniques: Titration, Concentration Assay, Fluorescence, Imaging, Expressing, Transduction