Journal: PLoS ONE

Article Title: The ACE2/Ang-(1-7)/Mas Axis Regulates the Development of Pancreatic Endocrine Cells in Mouse Embryos

doi: 10.1371/journal.pone.0128216

Figure Lengend Snippet: Primary antibodies used for immunofluorescence staining

Article Snippet: The fractionized-protein gel was transferred electrically to a PVDF membrane, which was first incubated with blocking solution (5% skim milk in PBS) for 1 h at room temperature to block nonspecific binding, and then probed with primary antibody (anti-ACE2 [NBP1-76614, 1:1000, Novus biologicals], anti-Mas1 receptor [NBP1-60091, 1:1000, Novus Biologicals], anti-p22 phox [sc-20781, 1:1000, Santa Cruz Biotechnology], and mouse monoclonal anti-β-actin IgG [sc-47778, 1:1500, Santa Cruz Biotechnology]; all primary antibodies are rabbit polyclonal unless otherwise stated).

Techniques: Immunofluorescence

Journal: Biomedicines

Article Title: Localization and Expression of Renin–Angiotensin System Receptors in Lung from Transplant Patients: A Case-Control Study

doi: 10.3390/biomedicines13092312

Figure Lengend Snippet: Total protein expression in lung homogenates of AT1, Mas, MrgD receptors was detected by immunoblotting using specific polyclonal antibodies. The molecular relative mass (kDa) is indicated to the right of the blot. Bar graphs represent the relative optical density of AT1: Angiotensin II Type 1 Receptor ( A ), Mas: Mas Receptor ( B ) and MrgD: Mas-Related G Protein-Coupled Receptor D ( C ) with total protein. Data presented are mean ± SD ( n = 9 control group and n = 10 FPI group); were analyzed using t -Test (* p < 0.05; *** p < 0.001).

Article Snippet: Antigen retrieval for anti-Mas1 Polyclonal antibody (ref. NBP1-60091, Novus Biologicals ® , Centennial, CO, USA) and anti-MRGPRF Polyclonal antibody (ref. PA5-110966, Thermo Fisher Scientific ® , Waltham, MA, USA) was performed in a water bath for 40 min at 95 °C in 20 mM Tris/EDTA buffer (pH 9.0).

Techniques: Expressing, Western Blot, Control

Journal: Biomedicines

Article Title: Localization and Expression of Renin–Angiotensin System Receptors in Lung from Transplant Patients: A Case-Control Study

doi: 10.3390/biomedicines13092312

Figure Lengend Snippet: Photomicrograph for localization of Mas and MrgD receptors. The positive immunohistochemical reaction is identified by brown color, observed by microscopy at 20× magnification and 40× magnification. In ( A ), control group and ( B ) FPI group, presence of the Mas receptor (Mas Polyclonal Antibody (Novus Biologicals ® , Centennial, CO, USA.—1:200) in the bronchiole. In ( C ), control group and ( D ) FPI group, presence of the MrgD receptor (MRGPRF Polyclonal Antibody (ThermoFisher Scientific ® , Waltham, MA, USA.—1:200), in the lung parenchyma.

Article Snippet: Antigen retrieval for anti-Mas1 Polyclonal antibody (ref. NBP1-60091, Novus Biologicals ® , Centennial, CO, USA) and anti-MRGPRF Polyclonal antibody (ref. PA5-110966, Thermo Fisher Scientific ® , Waltham, MA, USA) was performed in a water bath for 40 min at 95 °C in 20 mM Tris/EDTA buffer (pH 9.0).

Techniques: Immunohistochemical staining, Microscopy, Control