anti jnk  (Proteintech)

Journal: Oncology Reports

Article Title: Silencing of SIVA-1 promotes cisplatin resistance in gastric cancer via the Bcl-2/BAX-mediated mitochondria-dependent apoptosis pathway

doi: 10.3892/or.2026.9100

Figure Lengend Snippet: Analysis and validation of SIVA-1 interaction with Bcl-2, BAX, XIAP, MAPK8 and BIRC5, and related signaling pathways. (A) GSEA of KEGG pathways in the GSE186205 dataset. (B) GSEA of GO terms in the GSE186205 dataset. (C) BP, CC and MF terms associated with SIVA-1, Bcl-2, BAX, XIAP, MAPK8 and BIRC5, as determined by GO enrichment analysis in the GSE186205 dataset. (D) Gene expression correlation heatmap of SIVA-1, Bcl-2, BAX, XIAP, MAPK8 and BIRC5 in The Cancer Genome Atlas-stomach adenocarcinoma dataset. ‘X’ indicates P≥0.05. (E) Interactions between SIVA-1, and Bcl-2, BAX, XIAP, MAPK8 and BIRC5 proteins in the STRING 12.0 database. (F) Visual legend of the protein-protein interaction network. (G) mRNA expression levels of Bcl-2, BAX, XIAP, MAPK8 and BIRC5 in each group of cells, as detected by reverse transcription-quantitative PCR after SIVA-1 silencing. (H) Protein expression levels of Bcl-2, BAX, XIAP, MAPK8 and BIRC5, as detected by western blotting after SIVA-1 silencing in each group of cells. (I) Semi-quantification of the protein expression levels of Bcl-2, BAX, XIAP, MAPK8 and BIRC5 in different groups of cells (using GAPDH as an internal reference). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, as determined by ANOVA tests. BP, biological process; CC, cellular component; BIRC5, baculoviral inhibitor of apoptosis repeat-containing 5; GO, Gene Ontology; GSEA, Gene Set-Enrichment Analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; MF, molecular function; ns, not significant; XIAP, X-linked inhibitor of apoptosis protein.

Article Snippet: The following antibodies were procured from Cell Signaling Technology, Inc.: SIVA-1 (cat. no. 12532S), Bcl-2 (cat. no. 4223S), BAX (cat. no. 2772S), MAPK8 (cat. no. 3708S), BIRC5 (cat. no. 8756S), XIAP (cat. no. 2042S) and GAPDH (cat. no. 5174).

Techniques: Biomarker Discovery, Protein-Protein interactions, Gene Expression, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot

Journal: Frontiers in Immunology

Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

doi: 10.3389/fimmu.2026.1756467

Figure Lengend Snippet: Validation of the targeting relationship between let-7a-5p and MAPK8. (A) Schematic representation of the predicted complementary binding site between let-7a-5p and the 3′-UTR of MAPK8. (B) Relative mRNA expression level of MAPK8 in macrophages overexpressing let-7a-5p detected by RT-qPCR. (C) WB analysis of MAPK8 protein expression in macrophages overexpressing let-7a-5p. (D) Inhibitory effect of let-7a-5p on MAPK8 expression was assessed via a dual luciferase reporter assay. ( **P < 0.01, ***P < 0.001).

Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

Techniques: Biomarker Discovery, Binding Assay, Expressing, Quantitative RT-PCR, Luciferase, Reporter Assay

Journal: Frontiers in Immunology

Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

doi: 10.3389/fimmu.2026.1756467

Figure Lengend Snippet: Effect of PRP on expression of let-7a-5p and MAPK8. (A) Relative mRNA expression levels of let-7a-5p in knee joint sections of each group detected by RT-qPCR. (B) iNOS and CD206 co-immunolabeld with MAPK8 and counter-stained with DAPI in synovial tissues (Scale bar: 50 μm). (C, D) Quantification of iNOS and CD206 expression co-localized with MAPK8. ( **P < 0.01).

Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

Techniques: Expressing, Quantitative RT-PCR, Staining

Journal: Frontiers in Immunology

Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

doi: 10.3389/fimmu.2026.1756467

Figure Lengend Snippet: The let-7a-5p/MAPK8 axis regulates macrophage polarization and inflammatory cytokine release in vitro . (A) IF staining showing expression of iNOS and CD206 in macrophages (Scale bar: 50 μm). (B) Quantification of iNOS-positive cell rate in transfected macrophages. (C) Quantification of CD206-positive cell rate in transfected macrophages. (D-G) Relative mRNA expression levels of pro-inflammatory cytokine (IL-1β and TNF-α) and anti-inflammatory cytokine (IL-4 and IL-10) in transfected macrophages detected by RT-qPCR. ( *P < 0.05, **P < 0.01, ns no significance).

Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

Techniques: In Vitro, Staining, Expressing, Transfection, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

doi: 10.3390/ijms27010100

Figure Lengend Snippet: Comparison of MAP2K7, MAPK9, and MAPK8 gene expression and histological grade of CRC. * multiplication sign.

Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

Techniques: Comparison, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

doi: 10.3390/ijms27010100

Figure Lengend Snippet: Comparison between MAPK9 and MAPK8 expression and the nodal metastasis of CRC.

Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

Techniques: Comparison, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

doi: 10.3390/ijms27010100

Figure Lengend Snippet: Comparison of MAPK8 , MAP2K7 , and MAP2K4 gene expression between colon tumor tissue and normal colon tissue.

Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

Techniques: Comparison, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

doi: 10.3390/ijms27010100

Figure Lengend Snippet: Comparison of MAPK8 , MAP2K4 , and MAP2K7 gene expression with various clinicopathological features.

Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

Techniques: Comparison, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

doi: 10.3390/ijms27010100

Figure Lengend Snippet: Kaplan–Meier curves representing the survival rate of patients with colorectal cancer in relation to MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 expression.

Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

doi: 10.3390/ijms27010100

Figure Lengend Snippet: Differences in the expression of MAPK8, MAPK9, MAP2K4, and MAP2K7 genes in colorectal cancer tissue according to CMS. p -values were calculated using one-way ANOVA.

Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

Techniques: Expressing