inverted microscope nikon ti s whole cell macroscopic current recordings  (Nikon)

Journal: Purinergic Signalling

Article Title: Abnormal purinergic signaling contributes to development of renal cysts in autosomal dominant polycystic kidney disease

doi: 10.1007/s11302-025-10127-1

Figure Lengend Snippet: Testing inhibitory properties of SIL14 with patch-clamp. A Chemical structure of SIL14. B Representative current trace of pannexin-1 activity with 300 µM SIL14 application. Whole-cell recordings were obtained on CHO cells transiently transfected with mPanx1 under [90; −90] mV ramps. C Current–voltage characteristic of pannexin-1 conductance before and after SIL14 application. D Dose dependency of SIL14 effect on pannexin-1 measured at + 80 mV reveals IC 50 = 12.96 µM according to nonlinear regression analysis

Article Snippet: For each patch-clamp experiment, a glass chip with cells was transferred into the chamber with a constant flow of bath solution, placed under the inverted microscope Nikon Ti-S. Whole-cell macroscopic current recordings of pannexin-1 were made under voltage-clamp conditions with ramp protocol (90- > −90 mV).

Techniques: Patch Clamp, Activity Assay, Transfection