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Genechem lv-per2
Lv Per2, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lv-per2/per2+shrna+lentiviral+particles/pm39097147-151-70-81
Average 90 stars, based on 1 article reviews
lv-per2 - by Bioz Stars, 2026-09
90/100 stars

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Transduction:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.

Isolation:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.

Magnetic Beads:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.

Cell Culture:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.

In Vitro:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.

Transfection:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.

Infection:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.

Centrifugation:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.

Incubation:

Article Title: Circadian clock component PER2 negatively regulates CD4 + T cell IFN-γ production in ulcerative colitis.
Article Snippet: Lentiviral transduction of CD4+ T cells CD4+ T cells were isolated from peripheral blood samples using antihuman CD4 magnetic beads (BD Biosciences) and cultured in complete RPMI-1640 medium and stimulated in vitro with anti-CD28 mAb (2 μg/ ml, eBioscience) and anti-CD3 mAb (5 μg/ml, eBioscience) and for 48 h. The preactivated CD4+ T cells were reseeded at a density of 1 × 105/well in 24-well plates and then transfected with LV-PER2 (multiplicity of infection = 180, transcript ID: NM_022817, CDS: 64–1578) (Genechem; Shanghai, China), followed by centrifugation at 800 × g for 2 h at room temperature, then incubated for another 5 h at 37 ◦C.



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Genechem lv-per2
Lv Per2, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lv-per2/per2+shrna+lentiviral+particles/pm39097147-151-70-81
Average 90 stars, based on 1 article reviews
lv-per2 - by Bioz Stars, 2026-09
90/100 stars
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