lscm (Nikon)
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Nikon
lscm
Lscm, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lscm/Objectives/pmc12781939-112-6-7
Average 99 stars, based on 59766 article reviews
Lscm, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lscm/Objectives/pmc12781939-112-6-7
Average 99 stars, based on 59766 article reviews
lscm - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Cell swelling and upright mounting-based imaging for high-resolution visualization of intracellular trafficking across the BBB using conventional confocal microscopy"
Article Title: Cell swelling and upright mounting-based imaging for high-resolution visualization of intracellular trafficking across the BBB using conventional confocal microscopy
Journal: Drug Delivery
doi: 10.1080/10717544.2025.2608235
Figure Legend Snippet: Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) Confocal microscopy images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).
Techniques Used: Membrane, Confocal Microscopy, Staining, Control, Software
Figure Legend Snippet: Confocal microscopy analysis of early endosome trafficking patterns. (A) Cells treated with A647-Tf for 5 min, 15 min, or 1 h, stained with DAPI (blue), Rab5 (green, upper panels), and EEA1 (green, lower panels). (B) Cells treated with A647-anti-TfR Ab under the same conditions and staining. The red signal corresponds to internalized A647-Tf or A647-anti-TfR Ab. Scale bar: 10 μm.
Techniques Used: Confocal Microscopy, Staining
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