lrrc25  (Bioss)

Journal: Microorganisms

Article Title: LRRC25 Inhibits IFN-γ Secretion by Microglia to Negatively Regulate Anti-Tuberculosis Immunity in Mice

doi: 10.3390/microorganisms11102500

Figure Lengend Snippet: Increase in the transcription and translation of LRRC25 in H37Rv-infected BV2 cells. ( a ) The laser scanning confocal images GFP-H37Rv infects BV2 cells with MOIs of 1, 5, and 10 (n = 3). ( b ) Relative expression of LRRC25 mRNA after H37RV infection of BV2 cells for 4 h (n = 4), Student’s t test. ( c ) Western blot images of LRRC25 after H37RV infection of BV2 cells for 4 h (n = 3). ( d ) Cellular immunofluorescence image of LRRC25 H37RV-infected BV2 cells for 4 h. Scale bar, 100 μm. ( e ) H37Rv CFU count of mice brain tissue at 2 weeks, 4 weeks, and 6 weeks (n = 3), Kruskal–Wallis test. ( f ) HE staining images of the brain regions of mice infected with tuberculosis at 0, 2, 4, and 6 weeks (red arrows show the inflammatory cell infiltration areas. There were three mice in each group. Scale bar, 50 μm). ( g ) Immunofluorescence image of cerebellum of mice infected with H37Rv at 6 weeks; the nucleus is blue, IBA-1 is green, LRRC25 protein is red, and the scale is 50 μm (n = 3). The figure shows a representative result. *** p < 0.001.

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with LRRC25 antibody (LRRC25 antibody: Santa Cruz; 1:200).

Techniques: Infection, Expressing, Western Blot, Immunofluorescence, Staining

Journal: Microorganisms

Article Title: LRRC25 Inhibits IFN-γ Secretion by Microglia to Negatively Regulate Anti-Tuberculosis Immunity in Mice

doi: 10.3390/microorganisms11102500

Figure Lengend Snippet: Interference of the translation of LRRC25 using siRNA of LRRC25: L1, L2, and L3. ( a ) Relative expression of LRRC25 mRNA after transfection of BV2 cells for 48 h and 72 h (n = 4), One-way ANOVA. ( b ) Western blot images of LRRC25 after transfection of BV2 cells for 48 h and 72 h (n = 3). ( c ) Relative expression of LRRC25 mRNA in BV2 cells after being transfected for 48 h and 72 h and infected by GFP-H37Rv for 4 h at an MOI of 5 (n = 4), Student’s t test. ( d ) Western blot images of LRRC25 in BV2 cells after being transfected for 48 h and 72 h and infected by H37Rv for 4 h at an MOI of 5 (n = 3). ( e ) Histogram and percentage statistics of uninfected BV2 cells in flow cytometry (n = 3), One-way ANOVA, Ex post facto comparison -LSD. ( f ) Confocal images of infected BV2 cells. Among them, the nucleus was stained and marked with Hoechst (blue), and GFP-H37Rv was marked with green fluorescent protein (green). Scale bar, 50 μm. BV2 cells were transfected with L1 for 72 h and infected with GFP-H37Rv for 4 h at an MOI of 5. GA is the abbreviation for GAPDH, and NC is the abbreviation for Negative Control. *** p < 0.001.

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with LRRC25 antibody (LRRC25 antibody: Santa Cruz; 1:200).

Techniques: Expressing, Transfection, Western Blot, Infection, Flow Cytometry, Comparison, Staining, Negative Control

Journal: Microorganisms

Article Title: LRRC25 Inhibits IFN-γ Secretion by Microglia to Negatively Regulate Anti-Tuberculosis Immunity in Mice

doi: 10.3390/microorganisms11102500

Figure Lengend Snippet: LRRC25 prevents BV2 cells from releasing ISG15 and IFN-γ. ( a ) Relative expression of IL-1β mRNA of BV2 and secretion level of IL-1β in the cell supernatant of each group. ( b ) Levels of IFN-α, IFN-γ, and ISG15 in the cell culture supernatant before and after H37Rv infection of BV2 cells. ( c ) Relative expression of ISG15 mRNA and the expression of ISG15 in the cell supernatant in each group. ( d ) Expression of IFN-γ in the cell supernatant in each group. ( e ) Expression of IFN-α in the cell supernatant in each group. Bar graphs show the mean ± SD from four independent experiments (n = 4). Student’s t test for figure ( b ) and Two-way ANOVA test for figure ( a , c , d ). BV2 cells were transfected with L1 for 72 h and infected with GFP-H37Rv for 4 h at an MOI of 5. ** p < 0.01, and *** p < 0.001.

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with LRRC25 antibody (LRRC25 antibody: Santa Cruz; 1:200).

Techniques: Expressing, Cell Culture, Infection, Transfection

Journal: Microorganisms

Article Title: LRRC25 Inhibits IFN-γ Secretion by Microglia to Negatively Regulate Anti-Tuberculosis Immunity in Mice

doi: 10.3390/microorganisms11102500

Figure Lengend Snippet: Illustration of the negative regulation of anti-tuberculosis immunity in microglia by the LRRC25-ISG15-IFN-γ axis.

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with LRRC25 antibody (LRRC25 antibody: Santa Cruz; 1:200).

Techniques: