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lps  (MedChemExpress)


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    Structured Review

    MedChemExpress lps
    Lps, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lps/Lipopolysaccharides%2C+from+E%2E+coli+O55%3AB5/pmc13382133-24-0-2
    Average 99 stars, based on 680 article reviews
    lps - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Knockdown of DLGAP5 inhibits microglia activation in spinal cord injury rats through limiting NF-κB activation.
    Article Snippet: Background: Microglia-mediated neuroinflammation plays a significant role in the pathogenesis of spinal cord injury (SCI).. This study aimed to investigate the role of DLG associated protein 5 (DLGAP5) in SCI.. Methods: DLGAP5 expression was assessed in an SCI rat model using immunofluorescence, qRT-PCR, and Western blotting.

    other:

    Article Title: Mesoporous catechin hydrogel for enhanced astaxanthin-based inflammatory bowel disease therapy.
    Article Snippet: DCFH-DA (Cat. #: HY-D0940), LPS (Cat. #: HY-D1056) and DiR (Cat. #: HY-D1048) were purchased from MedChemExpress (Shanghai, China).


    Mouse Assay:

    Article Title: Tomentosin selectively targets microglial pyroptosis to overcome fluoxetine-resistant depression: a network-based therapeutic discovery
    Article Snippet: .. Mice were randomly assigned to four groups ( n = 8/group): saline, LPS (1 mg/kg, dissolved in normal saline; E. Coli O111:B4, L2630, Sigma-Aldrich, MO, USA), LPS with tomentosin, and LPS with BAY11-7082 (5 mg/kg, dissolved in normal saline; HY-13453, MCE, NJ, USA), respectively. ..

    Saline:

    Article Title: Tomentosin selectively targets microglial pyroptosis to overcome fluoxetine-resistant depression: a network-based therapeutic discovery
    Article Snippet: .. Mice were randomly assigned to four groups ( n = 8/group): saline, LPS (1 mg/kg, dissolved in normal saline; E. Coli O111:B4, L2630, Sigma-Aldrich, MO, USA), LPS with tomentosin, and LPS with BAY11-7082 (5 mg/kg, dissolved in normal saline; HY-13453, MCE, NJ, USA), respectively. ..



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    Image Search Results


    Effects of si- FAM30A on LPS-induced PDLSCs function and inflammatory levels. ( A) Exploration of optimal LPS concentration for inducing PDLSCs. ( B) Establishment of FAM30A knockdown cell lines. ( C) Effects of si- FAM30A transfection on PDLSCs proliferation. ( D) Apoptotic changes in PDLSCs following FAM30A inhibition. ( E) Expression of inflammatory-associated factors (TNF-α, IL-1β, IL-10) in PDLSCs following si- FAM30A transfection. ( F-H) Levels of oxidative stress markers malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) in PDLSCs after FAM30A inhibition. * P < .05 , ** P < .01 , *** P < .001 , **** P < .0001 .

    Journal: International Dental Journal

    Article Title: FAM30A Induces Inflammation and Oxidative Damage in PDLSCs by Targeting miR-424-5p

    doi: 10.1016/j.identj.2026.109605

    Figure Lengend Snippet: Effects of si- FAM30A on LPS-induced PDLSCs function and inflammatory levels. ( A) Exploration of optimal LPS concentration for inducing PDLSCs. ( B) Establishment of FAM30A knockdown cell lines. ( C) Effects of si- FAM30A transfection on PDLSCs proliferation. ( D) Apoptotic changes in PDLSCs following FAM30A inhibition. ( E) Expression of inflammatory-associated factors (TNF-α, IL-1β, IL-10) in PDLSCs following si- FAM30A transfection. ( F-H) Levels of oxidative stress markers malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) in PDLSCs after FAM30A inhibition. * P < .05 , ** P < .01 , *** P < .001 , **** P < .0001 .

    Article Snippet: PDLSCs were induced for 12 hours using 100 ng/mL lipopolysaccharide from E. coli O55:B5 (LPS, HY-D1056, MCE, U.S.) to establish an in vitro cellular model.

    Techniques: Concentration Assay, Knockdown, Transfection, Inhibition, Expressing

    ZDHHC17 deficiency stimulates pro-inflammatory cytokines production in macrophages leading to enhanced expression of fibro-genic genes in HSCs (A) THP-1 cells were induced to M0 macrophages by PMA and further polarized to M1 macrophages by treatment with LPS/IFN-γ. ZDHHC17 was over-expressed or knocked down in M1 macrophages by plasmid transfection or siRNA. Cells were collected 48 h post-transfection for detection. LX2 cells were seeded onto the lower chamber, and M1 macrophages were plated on the upper chamber of a 0.4 μm transwell membrane, which prevents direct cell contact, limiting cell interaction to secretory chemicals. (B and C) ZDHHC17 was significantly overexpressed in M1 macrophages by plasmid transfection (B) and decreased by small interfering RNAs (siRNAs) (C). Data are presented as the mean ± SD ( n = 6 independent experiments). mRNA expression of the pro-inflammatory cytokines (TNF-α, IL-1β, and IL-12β) were detected in ZDHHC17-overexpressed (D) or ZDHHC17-silenced (E) macrophages. Data are presented as the mean ± SD ( n = 6 independent experiments). ELISA detected serum TNF-α, IL-1β, and IL-12β in ZDHHC17-overexpressed (F) or ZDHHC17-silenced (G) macrophages. Data are presented as the mean ± SD ( n = 6 independent experiments). mRNA expression of α-SMA, COL1A1, TGF-β, and TIMP-1 was detected by qPCR in LX-2 cells following co-cultured with ZDHHC17-overexpressed (H) or silenced (I) macrophages. Data were presented as mean ± SD ( n = 6 independent experiments). ∗ p < 0.05 , ∗∗ p < 0.01 , ∗∗∗ p < 0.001 , ∗∗∗∗ p < 0.0001 by Student’s t test (B-I).

    Journal: iScience

    Article Title: ZDHHC17-mediated IKKβ palmitoylation suppresses NF-κB signaling to mitigate the progression of liver fibrosis

    doi: 10.1016/j.isci.2026.116360

    Figure Lengend Snippet: ZDHHC17 deficiency stimulates pro-inflammatory cytokines production in macrophages leading to enhanced expression of fibro-genic genes in HSCs (A) THP-1 cells were induced to M0 macrophages by PMA and further polarized to M1 macrophages by treatment with LPS/IFN-γ. ZDHHC17 was over-expressed or knocked down in M1 macrophages by plasmid transfection or siRNA. Cells were collected 48 h post-transfection for detection. LX2 cells were seeded onto the lower chamber, and M1 macrophages were plated on the upper chamber of a 0.4 μm transwell membrane, which prevents direct cell contact, limiting cell interaction to secretory chemicals. (B and C) ZDHHC17 was significantly overexpressed in M1 macrophages by plasmid transfection (B) and decreased by small interfering RNAs (siRNAs) (C). Data are presented as the mean ± SD ( n = 6 independent experiments). mRNA expression of the pro-inflammatory cytokines (TNF-α, IL-1β, and IL-12β) were detected in ZDHHC17-overexpressed (D) or ZDHHC17-silenced (E) macrophages. Data are presented as the mean ± SD ( n = 6 independent experiments). ELISA detected serum TNF-α, IL-1β, and IL-12β in ZDHHC17-overexpressed (F) or ZDHHC17-silenced (G) macrophages. Data are presented as the mean ± SD ( n = 6 independent experiments). mRNA expression of α-SMA, COL1A1, TGF-β, and TIMP-1 was detected by qPCR in LX-2 cells following co-cultured with ZDHHC17-overexpressed (H) or silenced (I) macrophages. Data were presented as mean ± SD ( n = 6 independent experiments). ∗ p < 0.05 , ∗∗ p < 0.01 , ∗∗∗ p < 0.001 , ∗∗∗∗ p < 0.0001 by Student’s t test (B-I).

    Article Snippet: LPS (Lipopolysaccharide) , MCE (Monmouth Junction, NJ, USA) , Cat# HY-D1056.

    Techniques: Expressing, Plasmid Preparation, Transfection, Membrane, Enzyme-linked Immunosorbent Assay, Cell Culture