Journal: Molecular Therapy Oncology
Article Title: Potency-enhancing mutations in E3-19K and i-leader increase the cytolytic activity of the PH20/ SPAM1 -armed oncolytic adenovirus Ad5Δ24RGD
doi: 10.1016/j.omton.2026.201137
Figure Lengend Snippet: The 19K SS and iL Q125Ter modifications mutually increase the potency of OAds regardless of the type of fiber modification (A) Sizes of MTT-stained plaques in cancer cell monolayers under an agarose overlay. Two to three independent experiments were conducted. The total number ( n ) of analyzed plaques is indicated for each group. Data are shown as means (SD) and analyzed using Brown-Forsythe and Welch ANOVA tests with Games-Howell post hoc multiple comparisons. (B) IC 50 values derived from viability curves after infection of cells (2.5 × 10 4 per well) in suspension with serial 3-fold dilutions of the indicated OAds. Data were collected on day 6 post-infection for all cell cultures except LN18 and LN229 cells infected with Ad5Δ24RGD-derived OAds (on day 7). Two to three independent experiments with technical triplicates were performed. Data are shown as means (SD). Only ≥3-fold differences from the parental virus Ad5Δ24RGD are indicated. (C) Sizes of fluorescent plaques and MTT-stained plaques in cancer cell monolayers under an agarose overlay. Three to four independent experiments were performed. The total number ( n ) of analyzed plaques is indicated for each group. Data are shown as means (SD) and analyzed using Brown-Forsythe and Welch ANOVA tests and Games-Howell post hoc multiple comparisons. ∗∗∗∗ p ≤ 0.0001; ∗∗∗ p ≤ 0.001; ∗∗ p ≤ 0.01; ∗ p ≤ 0.05; NS, not significant. See also .
Article Snippet: Human embryonic kidney HEK293, lung adenocarcinoma A549 and H441, prostate adenocarcinoma LNCap and PC3, hepatocellular carcinoma HepG2 and Hep3B, glioblastoma LN18, LN229, DBTRG, T98G, and U87 (American Type Culture Collection, ATCC), murine glioma CT-2A (#SCC194, Sigma) and GL261 cells (#ACC802, DSMZ Cell Culture Collection), and human short-term glioma (III–IV grade) primary cell cultures (GliSav, GliShat, GliBah, GliDu, AG-AASH, GBM100622, and GBM160323) were grown in Dulbecco’s modified Eagle medium (DMEM) with L-alanyl-glutamine, 4.5 g/L glucose, and Na pyruvate (#C415, Paneco), supplemented with 10% fetal bovine serum (FBS; HyClone/Cytiva, neoFroxx, or Capricorn Scientific) and penicillin-streptomycin solution at a final concentration of 50 U/mL and 50 μg/mL (#А063, Paneco).
Techniques: Modification, Staining, Derivative Assay, Infection, Suspension, Virus
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