human glioblastoma ln229  (ATCC)

Journal: Biochemistry and Biophysics Reports

Article Title: Development of novel anti-CDH1/E-cadherin monoclonal antibodies for versatile applications

doi: 10.1016/j.bbrep.2025.102401

Figure Lengend Snippet: Schematic representation of anti-CDH1 mAbs production. (A) LN229/CDH1 was injected intraperitoneally into BALB/cAJcl mice. (B) After five immunizations per week, spleen cells were fused with P3U1. (C) The supernatants from hybridomas were screened by flow cytometry using CHO/CDH1 and CHO–K1 cells. (D) Anti-CDH1 mAb-producing hybridoma clones (Ca 1 Mabs) were established through limiting dilution. LN229/CDH1, CDH1-overexpressed LN229; CHO, Chinese hamster ovary; CHO/CDH1, CDH1-overexpressed CHO–K1.

Article Snippet: Chinese hamster ovary (CHO)–K1, mouse myeloma P3X63Ag8U.1 (P3U1), human glioblastoma LN229, and human breast cancer MDA-MB-231 cell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Injection, Flow Cytometry, Clone Assay

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: CRISPR screening reveals differential drivers of sensitivity and resistance to TMZ and KL-50. ( A ) Schematic representing the mechanism of action of TMZ and KL-50. TMZ deposits methyl adducts while KL-50 deposits a fluoroethyl adduct that evolves into a DNA ICL. Created in BioRender. Heer, C. (2025) https://BioRender.com/uhifl36. ( B ) MAGeCK-RRA results from KL-50 screen. ( C ) Comparison of screening results between KL-50 and TMZ reveals differential involvement of MMR genes and BRIP1. ( D ) Western blot demonstrating CRISPR–Cas9-mediated knockout of BRIP1 in LN229 and RPE-1 cells. ( E and F ) Bar graph representing IC 50 values in WT and BRIP1/FANCJ KO LN229 cells treated with (E) KL-50 or (F) TMZ for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3–4, ratio paired t -test (one-tailed for KL-50, two-tailed for TMZ) ** P < 0.01, *** P < 0.001, n.s., not significant.

Article Snippet: LN229 (CRL-2611) and SW620 (CCL-227) cells were obtained from ATCC.

Techniques: CRISPR, Comparison, Western Blot, Knock-Out, One-tailed Test, Two Tailed Test

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Knockout of genes involved in TC-NER confers sensitivity to monofunctional alkylating agents. ( A and B ) MAGeCK-RRA results from illudin S and aflatoxin B1 screens. ( C ) Western blot demonstrating CRISPR–Cas9-mediated KO of ERCC8 in LN229 and SW620 cells. ( D and E ) Bar graph representing IC 50 values obtained of WT or ERCC8 KO LN229 and SW620 cells treated with (D) illudin S or (E) aflatoxin B1 for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3–4, ratio paired t -test (one-tailed) ** P < 0.01, *** P < 0.001.

Article Snippet: LN229 (CRL-2611) and SW620 (CCL-227) cells were obtained from ATCC.

Techniques: Knock-Out, Western Blot, CRISPR, One-tailed Test

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Knockout of genes involved in FA confers sensitivity to bifunctional and methylating agents. ( A–D ) MAGeCK-RRA results from bifunctional alkylating agents (A) cisplatin and (B) mitomycin C as well as methylating agents (C) TMZ and (D) MMS. ( E ) Western blot demonstrating CRISPR–Cas9-mediated knockout of FANCA and FANCD2 in LN229 and SW620 cells. ( F and G ) Bar graph representing IC 50 values from WT, FANCA KO, and FANCD2 KO LN229 and SW620 cells treated with (F) TMZ or (G) MMS for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3–4, ratio paired t -test (one-tailed) ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: LN229 (CRL-2611) and SW620 (CCL-227) cells were obtained from ATCC.

Techniques: Knock-Out, Western Blot, CRISPR, One-tailed Test

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Topoisomerase poisons topotecan and etoposide exhibit differential genetic dependencies. ( A and B ) MAGeCK-RRA results from topoisomerase poisons (A) topotecan and (B) etoposide. ( C ) Western blot demonstrating CRISPR–Cas9-mediated knockout of NHEJ1 in LN229 and SW620 cells. ( D and E ) Bar graph representing IC 50 values in WT and NHEJ1 KO LN229 (clone #7) and SW620 cells treated with (D) etoposide or (E) topotecan for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3, ratio paired t -test (one-tailed for etoposide, two-tailed for topotecan) * P < 0.05, *** P < 0.001, n.s., not significant.

Article Snippet: LN229 (CRL-2611) and SW620 (CCL-227) cells were obtained from ATCC.

Techniques: Western Blot, CRISPR, Knock-Out, One-tailed Test, Two Tailed Test

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Knockout of genes involved in TC-NER confers sensitivity to monofunctional alkylating agents. ( A and B ) MAGeCK-RRA results from illudin S and aflatoxin B1 screens. ( C ) Western blot demonstrating CRISPR–Cas9-mediated KO of ERCC8 in LN229 and SW620 cells. ( D and E ) Bar graph representing IC 50 values obtained of WT or ERCC8 KO LN229 and SW620 cells treated with (D) illudin S or (E) aflatoxin B1 for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3–4, ratio paired t -test (one-tailed) ** P < 0.01, *** P < 0.001.

Article Snippet: LN229 (CRL-2611) and SW620 (CCL-227) cells were obtained from ATCC.

Techniques: Knock-Out, Western Blot, CRISPR, One-tailed Test

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Knockout of genes involved in FA confers sensitivity to bifunctional and methylating agents. ( A–D ) MAGeCK-RRA results from bifunctional alkylating agents (A) cisplatin and (B) mitomycin C as well as methylating agents (C) TMZ and (D) MMS. ( E ) Western blot demonstrating CRISPR–Cas9-mediated knockout of FANCA and FANCD2 in LN229 and SW620 cells. ( F and G ) Bar graph representing IC 50 values from WT, FANCA KO, and FANCD2 KO LN229 and SW620 cells treated with (F) TMZ or (G) MMS for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3–4, ratio paired t -test (one-tailed) ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: LN229 (CRL-2611) and SW620 (CCL-227) cells were obtained from ATCC.

Techniques: Knock-Out, Western Blot, CRISPR, One-tailed Test

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Topoisomerase poisons topotecan and etoposide exhibit differential genetic dependencies. ( A and B ) MAGeCK-RRA results from topoisomerase poisons (A) topotecan and (B) etoposide. ( C ) Western blot demonstrating CRISPR–Cas9-mediated knockout of NHEJ1 in LN229 and SW620 cells. ( D and E ) Bar graph representing IC 50 values in WT and NHEJ1 KO LN229 (clone #7) and SW620 cells treated with (D) etoposide or (E) topotecan for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3, ratio paired t -test (one-tailed for etoposide, two-tailed for topotecan) * P < 0.05, *** P < 0.001, n.s., not significant.

Article Snippet: LN229 (CRL-2611) and SW620 (CCL-227) cells were obtained from ATCC.

Techniques: Western Blot, CRISPR, Knock-Out, One-tailed Test, Two Tailed Test

Journal: PeerJ

Article Title: The role of ASIC2 in glioma progression: implications for prognosis and therapeutic targeting

doi: 10.7717/peerj.20583

Figure Lengend Snippet: (A) Green fluorescent protein detection in LN229 and U87MG cell lines. (B) The detection of expression level of ASIC2 by shRNA plasmid. (C) Detection of ASIC2 expression level by shRNA plasmid. (D) Detection of ASIC2 expression level by overexpression plasmid. (E) Detection of cell proliferation ability with U87MG cells. (F) Detection of cell proliferation ability of LN229 cells in each group. (G) Detection of cell clone forming ability of U87MG and LN229 cells. (H) Statistical analysis of colony formation in U87MG and LN229 cells. (I) Detection of cell invasion ability of U87MG and LN229 cells. (J) Statistical analysis of the number of U87MG and LN229 cells migrating through the chamber. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01 vs control, # P < 0.05, ## P < 0.01 vs plasmid control.

Article Snippet: The human glioma cell line LN229 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, and the U87MG cell line was acquired from the American Type Culture Collection (ATCC).

Techniques: Expressing, shRNA, Plasmid Preparation, Over Expression, Control

Journal: PeerJ

Article Title: The role of ASIC2 in glioma progression: implications for prognosis and therapeutic targeting

doi: 10.7717/peerj.20583

Figure Lengend Snippet: (A) Detection of cell migration ability of glioma groups. (B) Statistical analysis of the wound closure rate in U87MG cells. (C) Statistical analysis of the wound closure rate in LN229 cells. (D–E) Regulation of cell cycle by ASIC2 on LN229 cells. (F, H) ASIC2 regulates the expression levels of P21, cycline D1 proteins in LN229. (G, I) Statistical analysis of protein expression in LN229 cells after ASIC2 interference and overexpression. (J–K) ASIC2 regulates the expression levels of MMP2, calcineurin and NFAT1 proteins in glioma cells. (L–O) Statistical analysis of protein expression in U87MG and LN229 cells after ASIC2 interference and overexpression. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 vs control, # P < 0.05, ## P < 0.01, ### P < 0.001 vs plasmid control.

Article Snippet: The human glioma cell line LN229 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, and the U87MG cell line was acquired from the American Type Culture Collection (ATCC).

Techniques: Migration, Expressing, Over Expression, Control, Plasmid Preparation