ll97a (ATCC)

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ATCC ll97a

Determination of cytotoxicity of anti-GPC1 mAb in 2D monocultures and 3D monoculture and coculture spheroids. (A) 2D A549 monocultures; (B) 2D H460 monocultures; (C) 2D <t>LL97A</t> monocultures; (D) 3D A549 monocultures and A549/LL97A cocultures; (E) H460 monocultures and H460/LL97A cocultures. Cells were treated with anti-GPC1 mAb and mouse IgG isotype at a range of concentration. IC50 values were based on the concentration-effect curves. Results are presented as mean ± SD ( n = 4 for 2D cultures and n = 3 for 3D cultures). Error bars represent the inter-assay SD. ** P < 0.01 compared with anti-GPC1 mAb treated 2D H460 monocultures using one-way ANOVA followed by Tukey-Kramer post hoc multiple comparison test. ++ P < 0.01 compared with the corresponding 3D monoculture counterpart using independent two-sample t -test. GPC1: glypican-1; mAb: monoclonal antibody; IgG: immunoglobulin G

Ll97a, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ll97a/LL+97A/pmc11220310-29-25-33
Average 94 stars, based on 54 article reviews

ll97a - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Evaluation of antitumor potential of an anti-glypican-1 monoclonal antibody in preclinical lung cancer models reveals a distinct mechanism of action"

Article Title: Evaluation of antitumor potential of an anti-glypican-1 monoclonal antibody in preclinical lung cancer models reveals a distinct mechanism of action

Journal: Exploration of Targeted Anti-tumor Therapy

doi: 10.37349/etat.2024.00238

Figure Legend Snippet: Determination of cytotoxicity of anti-GPC1 mAb in 2D monocultures and 3D monoculture and coculture spheroids. (A) 2D A549 monocultures; (B) 2D H460 monocultures; (C) 2D LL97A monocultures; (D) 3D A549 monocultures and A549/LL97A cocultures; (E) H460 monocultures and H460/LL97A cocultures. Cells were treated with anti-GPC1 mAb and mouse IgG isotype at a range of concentration. IC50 values were based on the concentration-effect curves. Results are presented as mean ± SD ( n = 4 for 2D cultures and n = 3 for 3D cultures). Error bars represent the inter-assay SD. ** P < 0.01 compared with anti-GPC1 mAb treated 2D H460 monocultures using one-way ANOVA followed by Tukey-Kramer post hoc multiple comparison test. ++ P < 0.01 compared with the corresponding 3D monoculture counterpart using independent two-sample t -test. GPC1: glypican-1; mAb: monoclonal antibody; IgG: immunoglobulin G

Techniques Used: Concentration Assay, Inter Assay, Comparison

Western blot analysis of monocultured LL97A, A549 and H460 cells after treatments. Western blot images demonstrated the protein expression of total and phosphorylated mTOR, RSK1/2/3, FAK, Src Akt, GSK3α/3β, ERK1/2 and MEK1/2, selected EMT markers and GPC1 protein in monocultured LL97A lung fibroblasts, A549 and H460 tumor cells treated with vehicle control, 100 μg/mL mouse IgG isotype, or 100 μg/mL anti-GPC1 mAb for 24 h. Numbers below individual blots represent the percentage of the normalized integrated density values against β-actin (loading control). C: control; I: mouse IgG; G: anti-GPC1 mAb. GPC1: glypican-1; p-FAK: phospho-focal adhesion kinase; p90RSK: 90 kDa ribosomal s6 kinase; GSK3α: glycogen synthase kinases 3α; ERK: extracellular signal-regulated kinase; MEK: mitogen-activated protein kinase kinase; GPC1: glypican-1

Figure Legend Snippet: Western blot analysis of monocultured LL97A, A549 and H460 cells after treatments. Western blot images demonstrated the protein expression of total and phosphorylated mTOR, RSK1/2/3, FAK, Src Akt, GSK3α/3β, ERK1/2 and MEK1/2, selected EMT markers and GPC1 protein in monocultured LL97A lung fibroblasts, A549 and H460 tumor cells treated with vehicle control, 100 μg/mL mouse IgG isotype, or 100 μg/mL anti-GPC1 mAb for 24 h. Numbers below individual blots represent the percentage of the normalized integrated density values against β-actin (loading control). C: control; I: mouse IgG; G: anti-GPC1 mAb. GPC1: glypican-1; p-FAK: phospho-focal adhesion kinase; p90RSK: 90 kDa ribosomal s6 kinase; GSK3α: glycogen synthase kinases 3α; ERK: extracellular signal-regulated kinase; MEK: mitogen-activated protein kinase kinase; GPC1: glypican-1

Techniques Used: Western Blot, Expressing, Control

Western blot analysis of co-cultured LL97A, A549 and H460 cells after treatments. Western blot images demonstrated the expression of total and phosphorylated mTOR, RSK1/2/3, FAK, Src Akt, GSK3α/3β, ERK1/2 and MEK1/2, selected EMT markers and GPC1 protein in LL97A lung fibroblasts, A549 and H460 tumor cells that were co-cultured in a transwell indirect co-culture system and treated with vehicle control, 100 μg/mL mouse IgG isotype, or 100 μg/mL anti-GPC1 mAb for 24 h. Numbers below individual blots represent the percentage of the normalized integrated density values against β-actin (loading control). C: vehicle control; I: 100 μg/mL mouse IgG isotype; G: 100 μg/mL anti-GPC1 mAb. p-FAK: phospho-focal adhesion kinase; p90RSK: 90 kDa ribosomal s6 kinase; GSK3α: glycogen synthase kinases 3α; ERK: extracellular signal-regulated kinase; MEK: mitogen-activated protein kinase kinase; GPC1: glypican-1

Figure Legend Snippet: Western blot analysis of co-cultured LL97A, A549 and H460 cells after treatments. Western blot images demonstrated the expression of total and phosphorylated mTOR, RSK1/2/3, FAK, Src Akt, GSK3α/3β, ERK1/2 and MEK1/2, selected EMT markers and GPC1 protein in LL97A lung fibroblasts, A549 and H460 tumor cells that were co-cultured in a transwell indirect co-culture system and treated with vehicle control, 100 μg/mL mouse IgG isotype, or 100 μg/mL anti-GPC1 mAb for 24 h. Numbers below individual blots represent the percentage of the normalized integrated density values against β-actin (loading control). C: vehicle control; I: 100 μg/mL mouse IgG isotype; G: 100 μg/mL anti-GPC1 mAb. p-FAK: phospho-focal adhesion kinase; p90RSK: 90 kDa ribosomal s6 kinase; GSK3α: glycogen synthase kinases 3α; ERK: extracellular signal-regulated kinase; MEK: mitogen-activated protein kinase kinase; GPC1: glypican-1

Techniques Used: Western Blot, Cell Culture, Expressing, Co-Culture Assay, Control

Semi-quantitation of protein expression in monoculture and coculture samples determined by Western blot analysis. (A) LL97A monocultures; (B) LL97A fibroblasts in indirect coculture with H460 cells; (C) LL97A fibroblasts in indirect coculture with A549 cells; (D) A549 cells in indirect coculture with LL97A fibroblasts. Effects of anti-GPC1 mAb on the expression levels of selected EMT markers and effector proteins common to the GPC1-facilitated growth factor signal transduction pathways, including MEK/ERK/RSK, Akt/GSK3/mTOR, Src/FAK pathways were determined using Western blot analysis. Cell monocultures or co-cultures were treated with vehicle (i.e., PBS), 100 μg/mL mouse IgG isotype and 100 μg/mL anti-GPC1-mAb for 24 h. Whole cell lysate samples were subjected to Western blot analysis (30 μg of protein per lane). Detection of β-actin was used to ensure equal sample loading per lane. Relative immunoreactive band intensities are expressed as percent change over the average signal value in the vehicle-treated cells. For phosphorylated proteins, results are expressed as the ratio of phosphorylated-to-total species relative to the vehicle control cells. Data are representative of three independent experiments ( n = 3) and presented as mean ± SD. SD is denoted by the error bars. * P < 0.05 and ** P < 0.01 compared among vehicle, 100 μg/mL mouse IgG isotype and 100 μg/mL anti-GPC1-mAb in the same cell line using one-way ANOVA followed by Tukey-Kramer post hoc multiple comparison test. GPC1: glypican-1; p-FAK: phospho-focal adhesion kinase; GSK3α: glycogen synthase kinases 3α; MEK: mitogen-activated protein kinase kinase; ERK: extracellular signal-regulated kinase; p90RSK: 90 kDa ribosomal s6 kinase

Figure Legend Snippet: Semi-quantitation of protein expression in monoculture and coculture samples determined by Western blot analysis. (A) LL97A monocultures; (B) LL97A fibroblasts in indirect coculture with H460 cells; (C) LL97A fibroblasts in indirect coculture with A549 cells; (D) A549 cells in indirect coculture with LL97A fibroblasts. Effects of anti-GPC1 mAb on the expression levels of selected EMT markers and effector proteins common to the GPC1-facilitated growth factor signal transduction pathways, including MEK/ERK/RSK, Akt/GSK3/mTOR, Src/FAK pathways were determined using Western blot analysis. Cell monocultures or co-cultures were treated with vehicle (i.e., PBS), 100 μg/mL mouse IgG isotype and 100 μg/mL anti-GPC1-mAb for 24 h. Whole cell lysate samples were subjected to Western blot analysis (30 μg of protein per lane). Detection of β-actin was used to ensure equal sample loading per lane. Relative immunoreactive band intensities are expressed as percent change over the average signal value in the vehicle-treated cells. For phosphorylated proteins, results are expressed as the ratio of phosphorylated-to-total species relative to the vehicle control cells. Data are representative of three independent experiments ( n = 3) and presented as mean ± SD. SD is denoted by the error bars. * P < 0.05 and ** P < 0.01 compared among vehicle, 100 μg/mL mouse IgG isotype and 100 μg/mL anti-GPC1-mAb in the same cell line using one-way ANOVA followed by Tukey-Kramer post hoc multiple comparison test. GPC1: glypican-1; p-FAK: phospho-focal adhesion kinase; GSK3α: glycogen synthase kinases 3α; MEK: mitogen-activated protein kinase kinase; ERK: extracellular signal-regulated kinase; p90RSK: 90 kDa ribosomal s6 kinase

Techniques Used: Quantitation Assay, Expressing, Western Blot, Transduction, Control, Comparison

Cell Culture:

Article Title: Oxy210, a Semi-Synthetic Oxysterol, Inhibits Profibrotic Signaling in Cellular Models of Lung and Kidney Fibrosis.
Article Snippet: .. Cell Culture and Reagents IMR-9 , LL97A, human pericyte, human renal fibroblast cells, and human renal mesangial cells were obtained from ATCC (Manassas, VA, USA). ..

Article Title: Alvespimycin Exhibits Potential Anti-TGF-β Signaling in the Setting of a Proteasome Activator in Rats with Bleomycin-Induced Pulmonary Fibrosis: A Promising Novel Approach
Article Snippet: .. The well-defined and cultured human lung fibroblast lines WI-38 and LL97A were acquired from ATCC in Manassas, USA. .. They were then cultivated in high-glucose DMEM (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS, 2 mM glutamine, 50 μg/mL streptomycin, and 50 U/mL penicillin.

Isolation:

Article Title: miRNAs Contained in Extracellular Vesicles Cargo Contribute to the Progression of Idiopathic Pulmonary Fibrosis: An In Vitro Aproach
Article Snippet: .. The human lung fibroblast cell lines LL29 and LL97A, isolated from IPF patients, were obtained from the American Type Culture Collection (ATCC, Cat. No. CCL-134 and CCL-191, respectively; Manassas, VA, USA), and CCD19 cells were considered as normal control (ATCC, Cat. No. CCL-210; Manassas, VA, USA). .. All cells were cultured in Eagle’s minimal essential medium (EMEM; ATCC No. 30-2003; Manassas, VA, USA) supplemented with 10% FBS (26140079, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 100 U/mL penicillin/streptomycin (15140122, Gibco, Thermo Fisher Scientific, Waltham, MA, USA).

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ll97a (ATCC)

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1) Product Images from "Evaluation of antitumor potential of an anti-glypican-1 monoclonal antibody in preclinical lung cancer models reveals a distinct mechanism of action"

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