other:Article Title: A role for CASM in the repair of damaged Golgi architecture
Article Snippet: Primary antibodies were obtained as follows: mCherry (ab183628), GABARAP (ab109364; 1:500 for IF), and NPC2 (ab2181921:1,000 for WB) from Abcam; AMPK (2532; 1:1000 for WB), phospho-AMPK T172 (2535; 1:1,000 for WB), ATG13 (13468; 1:1,000 for WB), BECN1 (3459; 1:1,000 for WB), GM130 (12480; 1:500 for IF, 1:1,000 for WB), mTOR (2983; 1:500 for IF), phospho-P70 S6K (9205; 1:1,000 for WB), TFEB (4240; 1:500 for IF), and ULK1 (8054; 1:1,000 for WB) from Cell Signaling Technology; actin (sc-58673; 1:1,000 for WB), Cas9 (sc-517386, 1:1000 for WB), LAMP2 (sc-18822; 1:1,000 for WB, 1:500 for IF), P70 S6K (sc-8418; 1:1,000 for WB), and alpha-tubulin (sc-23948; 1:500 for IF) from Santa Cruz Biotechnology; Cathepsin D (21327-1-AP, 1:1,000 for WB), myc (16286-1-AP: 1:1,000 for WB), LIMP2 (27102-1-AP, 1:1,000 for WB), TRIM46(21026-1-AP, 1:1,000 for WB) from Proteintech; LC3B (PM036; 1:500 for IF) from MBL and LC3B (L7543; 1:2,000 for WB) from Sigma; p62/SQSTM1(610833; 1:2,000 for WB) from BD; ATG7 (MA5-32221; 1:1,000 for WB), GCP60 (MA5-25999; 1:1,000 for WB), TGN46 (MA5-37930; 1:1,000 for WB), and WIPI1 (PA5-34973; 1:1000 for WB) from Thermo Fisher and TGN46 (AHP500G; 1:500 for IF) from Bio-Rad.
Western Blot:Article Title: Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo
Article Snippet: .. The following primary antibodies were used in this study: goat anti LIMP2 (Cat# AF1966-SP,1:2000), mouse anti ACT2 (Cat# A5316, 1:4000), and rabbit anti LAMTOR1 (Cat # HPA002997, 1:1000) from Sigma-Aldrich; mouse anti CANX (Cat# 66903-1-AP, 1:20,000), mouse anti FLOT2 (Cat# 66881-1-Ig, 1:1500), mouse anti FZD9 (Cat# 67023-1-Ig, 1:1500), rabbit anti ATP6V1D (Cat# 14920-1-AP, 1:1000), rabbit anti CTSD (Cat# # 21327-1-AP, 1:1000), rabbit anti FLOT1 (Cat# 15571-1-AP, 1:2000), rabbit anti GNB4 (Cat# 11978-2-AP, 1:2000), and rabbit anti SDHA (Cat# 14865-1-AP, 1:800) from Proteintech; goat anti LIMP2 (Cat# AF1966-SP, 1:2000) from R&D system; mouse anti ATP6V1B2 (Cat# SC166045, 1:1000) from Santa Cruz; mouse anti FLOT1 (Cat# 610821, 1:200), mouse anti FLOT2 (Cat# 610383, 1:200), and mouse anti GM130 (Cat# 610822) from BD Biosciences; mouse anti GAPDH (Cat# 5174, 1:2500), rabbit anti EEA1 (Cat# 2411, 1:200), rabbit anti FLOT1 (Cat# 18634, 1:200), and rabbit anti RRAGA (Cat# 4357, 1:1000) from Cell signaling; rabbit anti LPHN2 (Cat# NBP2-58704, 1:100) and rabbit anti LPHN3 (Cat# NLS1138, 1:200) from Novus Biologicals; goat anti-mouse IgG (H + L)-Alexa Fluor 488 (Cat# A-11029, 1:400), rabbit anti ATP6V1A1 (Cat# PA5-29191,1:2000), and rabbit anti LPHN1 (Cat # PA5-77475, 1:200) from Thermo Fisher Scientific; rabbit anti TUBA (Cat# 600-401-880, 1:2000) from Rockland; mouse anti LAMP2 (Cat# H4B4, 1:1000 for WB, 1:100 for IF) from Hybridoma Bank. rabbit anti DSSO (Self-made , 1:5000). .. The following secondary antibodies were used in this study: rat anti-FLAG (Cat# SAB4200119,1:10,000) from Sigma-Aldrich; HRP-coupled goat anti-mouse IgG (Cat# 115-035-044, 1:5000), Cy3-coupled goat anti rabbit IgG (H + L) (Cat# 111-165-144, 1:400), and HRP-coupled goat anti rabbit IgG (Cat# 111-035-003, 1:5000) from Dianova.
Article Title: Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo.
Article Snippet: .. The following primary antibodies were used in this study: goat anti LIMP2 (Cat# AF1966-SP,1:2000), mouse anti ACT2 (Cat# A5316, 1:4000), and rabbit anti LAMTOR1 (Cat # HPA002997, 1:1000) from Sigma-Aldrich; mouse anti CANX (Cat# 66903-1-AP, 1:20,000), mouse anti FLOT2 (Cat# 66881-1-Ig, 1:1500), mouse anti FZD9 (Cat# 67023-1- Ig, 1:1500), rabbit anti ATP6V1D (Cat# 14920-1-AP, 1:1000), rabbit anti CTSD (Cat# # 21327-1-AP, 1:1000), rabbit anti FLOT1 (Cat# 15571-1-AP, 1:2000), rabbit anti GNB4 (Cat# 11978-2-AP, 1:2000), and rabbit anti SDHA (Cat# 14865-1-AP, 1:800) from Proteintech; goat anti LIMP2 (Cat# AF1966-SP, 1:2000) from R&D system; mouse anti ATP6V1B2 (Cat# SC166045, 1:1000) from Santa Cruz; mouse anti FLOT1 (Cat# 610821, 1:200), mouse anti FLOT2 (Cat# 610383, 1:200), and mouse anti GM130 (Cat# 610822) from BD Biosciences; mouse anti GAPDH (Cat# 5174, 1:2500), rabbit anti EEA1 (Cat# 2411, 1:200), rabbit anti FLOT1 (Cat# 18634, 1:200), and rabbit anti RRAGA (Cat# 4357, 1:1000) from Cell signaling; rabbit anti LPHN2 (Cat# NBP2-58704, 1:100) and rabbit anti LPHN3 (Cat# NLS1138, 1:200) from Novus Biologicals; goat anti-mouse IgG (H + L)-Alexa Fluor 488 (Cat# A-11029, 1:400), rabbit anti ATP6V1A1 (Cat# PA5-29191,1:2000), and rabbit anti LPHN1 (Cat # PA5-77475, 1:200) from Thermo Fisher Scientific; rabbit anti TUBA (Cat# 600-401-880, 1:2000) from Rockland; mouse anti LAMP2 (Cat# H4B4, 1:1000 for WB, 1:100 for IF) from Hybridoma Bank. rabbit anti DSSO (Self-made35, 1:5000). .. The following secondary antibodies were used in this study: rat anti-FLAG (Cat# SAB4200119,1:10,000) from Sigma-Aldrich; HRP-coupled goat anti-mouse IgG (Cat# 115-035-044, 1:5000), Cy3-coupled goat anti rabbit IgG (H+ L) (Cat# 111-165-144, 1:400), and HRP-coupled goat anti rabbit IgG (Cat# 111-035-003, 1:5000) from Dianova.
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