Journal: American journal of physiology. Gastrointestinal and liver physiology
Article Title: Impaired intestinal cell proliferation parallels increased senescence after burn injury in aged mice
doi: 10.1152/ajpgi.00176.2025
Figure Lengend Snippet: Expression of crypt-base columnar stem cell marker lgr5 in the (A) colon, (B) IECs, and (C) organoids and reserve stem cell marker sox9 in the (D) colon, (E) IECs, and (F) organoids measured by RT-qPCR across all groups shown as relative expression normalized to gapdh . *p<0.05 from all other groups, +p<0.05 from Young Burn, ^p<0.05 from Young Sham by one-way ANOVA with Tukey’s post-hoc test. n=3–10 mice combined from two independent experiments.
Article Snippet: Quantitative RT-PCR was performed using TaqMan probes, ang4 (Mm03647554_g1), camp (Mm00438285_m1), cdk4 (Mm00677718_g1), cdkn2a (Mm00494449_m1), lgr5 (Mm00438890_m1), and sox9 (Mm00448840_m1), (Applied Biosystems) and TaqMan Universal PCR Mastermix (cat#4364340, Applied Biosystems) and run on a QuantStudio 3 Real-Time PCR System (Applied Biosystems).
Techniques: Expressing, Marker, Quantitative RT-PCR