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Proteintech lc3b
Lc3b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3b/LC3+Antibody/pm42873876-319-14-15
Average 96 stars, based on 2142 article reviews
lc3b - by Bioz Stars, 2026-11
96/100 stars

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Related Articles

Blocking Assay:

Article Title: A H 2 S-releasing oridonin derivative improves diabetic wound healing based on antibacterial activity and regulation of macrophage phagocytosis.
Article Snippet: .. After blocking with 5% skimmed milk powder in TBST (0.1% Tween 20) for 2 h at room temperature, all blots were incubated with the following primary antibodies: TLR2 (1:2000, Huabio, Hangzhou, China), LC3B (1:1000, Proteintech, Wuhan, China), P62 (1:3000, Proteintech, Wuhan,China), Beclin-1 (1:1000, Proteintech, Wuhan, China), and β-actin (1:2000, Huabio, Hangzhou, China), overnight at 4 ◦C, and then the secondary antibodies were coupled with horseradish peroxidase (HRP) and visualised by chemiluminescence (ECL) detection kit, followed by radiographic autoradiography. ..

Article Title: E7-engineered gastrodiae rhizoma-derived nanovesicles promote diabetic bone regeneration with the involvement of PINK1/parkin-mediated mitophagy
Article Snippet: Twenty micrograms of protein sample were separated by SDS-PAGE and transferred to a 0.22 μm PVDF membrane (Merck Millipore, USA). .. After blocking, the membrane was incubated overnight at 4 ◦C with the following primary antibodies: OPN (1:1000, CST), OSX (1:2000, Abcam), BMP2 (1:1000, Thermo Fisher Scientific), RUNX2 (1:1000, Abcam), Parkin (1:1000, Abcam), PINK1 (1:1000, Abcam), LC3B (1:1000, Proteintech), p62 (1:1000, Abcam), and β-actin (1:10000, Abcam). .. Subsequently, the membranes were incubated with the corresponding secondary antibodies (Abcam, UK) at room temperature for 1 h. Finally, the target band signals were detected using a chemiluminescent imaging system (BioRad, USA).

Incubation:

Article Title: A H 2 S-releasing oridonin derivative improves diabetic wound healing based on antibacterial activity and regulation of macrophage phagocytosis.
Article Snippet: .. After blocking with 5% skimmed milk powder in TBST (0.1% Tween 20) for 2 h at room temperature, all blots were incubated with the following primary antibodies: TLR2 (1:2000, Huabio, Hangzhou, China), LC3B (1:1000, Proteintech, Wuhan, China), P62 (1:3000, Proteintech, Wuhan,China), Beclin-1 (1:1000, Proteintech, Wuhan, China), and β-actin (1:2000, Huabio, Hangzhou, China), overnight at 4 ◦C, and then the secondary antibodies were coupled with horseradish peroxidase (HRP) and visualised by chemiluminescence (ECL) detection kit, followed by radiographic autoradiography. ..

Article Title: E7-engineered gastrodiae rhizoma-derived nanovesicles promote diabetic bone regeneration with the involvement of PINK1/parkin-mediated mitophagy
Article Snippet: Twenty micrograms of protein sample were separated by SDS-PAGE and transferred to a 0.22 μm PVDF membrane (Merck Millipore, USA). .. After blocking, the membrane was incubated overnight at 4 ◦C with the following primary antibodies: OPN (1:1000, CST), OSX (1:2000, Abcam), BMP2 (1:1000, Thermo Fisher Scientific), RUNX2 (1:1000, Abcam), Parkin (1:1000, Abcam), PINK1 (1:1000, Abcam), LC3B (1:1000, Proteintech), p62 (1:1000, Abcam), and β-actin (1:10000, Abcam). .. Subsequently, the membranes were incubated with the corresponding secondary antibodies (Abcam, UK) at room temperature for 1 h. Finally, the target band signals were detected using a chemiluminescent imaging system (BioRad, USA).

Article Title: E7-engineered gastrodiae rhizoma-derived nanovesicles promote diabetic bone regeneration with the involvement of PINK1/parkin-mediated mitophagy
Article Snippet: .. Subsequently, sections were blocked with 5% goat serum at room temperature for 1 h, then incubated overnight at 4 ◦C with primary antibodies against ALP(5 μg/mL, Thermo Fisher Scientific), LC3B(1:500, Proteintech), and TOM20 (1:500, Thermo Fisher Scientific). .. After thorough washing with PBS, sections were incubated with corresponding secondary antibodies (Invitrogen, USA) for 1 h at room temperature in the dark.

Article Title: miR-20a-5p inhibition attenuates post-myocardial infarction ventricular remodeling in association with mTORC1/S6 signaling
Article Snippet: Protein samples (20–40 μg) were separated by SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes (IPVH00010, Millipore, USA). .. Membranes were incubated with antibodies against Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-73364-3 p-S6 (1:2,000, 80130-2-RR, Proteintech), LC3B (1:6,000, 14600-1-AP, Proteintech), p62 (1:10,000, 84826-1- RR, Proteintech), cleaved caspase-3 (1:1,000, #9661, CST), Bcl-2 (1:500, 26593-1-AP, Proteintech), α-SMA (1:1,000, 19245 S, CST), Gal-3 (1:1,000, #89572, CST), and GAPDH (1:5,000, 10494-1-AP, Proteintech), followed by an HRP-conjugated secondary antibody (1:3,000, RGAR001, Proteintech). .. Signals were developed using ECL reagent (34577, Thermo Fisher Scientific, USA) and quantified using Image Lab software.

Autoradiography:

Article Title: A H 2 S-releasing oridonin derivative improves diabetic wound healing based on antibacterial activity and regulation of macrophage phagocytosis.
Article Snippet: .. After blocking with 5% skimmed milk powder in TBST (0.1% Tween 20) for 2 h at room temperature, all blots were incubated with the following primary antibodies: TLR2 (1:2000, Huabio, Hangzhou, China), LC3B (1:1000, Proteintech, Wuhan, China), P62 (1:3000, Proteintech, Wuhan,China), Beclin-1 (1:1000, Proteintech, Wuhan, China), and β-actin (1:2000, Huabio, Hangzhou, China), overnight at 4 ◦C, and then the secondary antibodies were coupled with horseradish peroxidase (HRP) and visualised by chemiluminescence (ECL) detection kit, followed by radiographic autoradiography. ..

Membrane:

Article Title: E7-engineered gastrodiae rhizoma-derived nanovesicles promote diabetic bone regeneration with the involvement of PINK1/parkin-mediated mitophagy
Article Snippet: Twenty micrograms of protein sample were separated by SDS-PAGE and transferred to a 0.22 μm PVDF membrane (Merck Millipore, USA). .. After blocking, the membrane was incubated overnight at 4 ◦C with the following primary antibodies: OPN (1:1000, CST), OSX (1:2000, Abcam), BMP2 (1:1000, Thermo Fisher Scientific), RUNX2 (1:1000, Abcam), Parkin (1:1000, Abcam), PINK1 (1:1000, Abcam), LC3B (1:1000, Proteintech), p62 (1:1000, Abcam), and β-actin (1:10000, Abcam). .. Subsequently, the membranes were incubated with the corresponding secondary antibodies (Abcam, UK) at room temperature for 1 h. Finally, the target band signals were detected using a chemiluminescent imaging system (BioRad, USA).

ALP Assay:

Article Title: E7-engineered gastrodiae rhizoma-derived nanovesicles promote diabetic bone regeneration with the involvement of PINK1/parkin-mediated mitophagy
Article Snippet: .. Subsequently, sections were blocked with 5% goat serum at room temperature for 1 h, then incubated overnight at 4 ◦C with primary antibodies against ALP(5 μg/mL, Thermo Fisher Scientific), LC3B(1:500, Proteintech), and TOM20 (1:500, Thermo Fisher Scientific). .. After thorough washing with PBS, sections were incubated with corresponding secondary antibodies (Invitrogen, USA) for 1 h at room temperature in the dark.



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Image Search Results


Antibodies.

Journal: Science Advances

Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy

doi: 10.1126/sciadv.aef8820

Figure Lengend Snippet: Antibodies.

Article Snippet: Western blot (mouse sample) , Novus Biologicals , NB100-2220.

Techniques: Western Blot