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lamp3  (Proteintech)


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    Structured Review

    Proteintech lamp3
    Lamp3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamp3/LAMP3+Antibody/pm42880512-142-9-11
    Average 93 stars, based on 33 article reviews
    lamp3 - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Single-cell RNA sequencing reveals the adverse role of cDC3s in the response of ulcerative colitis patients to anti-TNF-α therapy
    Article Snippet: .. Immunofluorescence staining was performed in a darkroom using DAPI (catalog: G1012-100, Servicebio), LAMP3 (catalog: 12632-1-AP, Proteintech, dilution ratio of 1:100), and HLA-DPB1 (catalog: bs-1198R, Bioss, dilution ratio of 1:100) antibodies. .. After incubation at room temperature, the sections were rinsed with PBS (glycerin:PBS = 1:1) and mounted for observation via a laser digital scan of the tissue sections (catalog: Pannorimic MIDI, 3DHISTECH) to assess the intracellular localization of the target proteins.

    Article Title: Single-cell RNA sequencing reveals the adverse role of cDC3s in the response of ulcerative colitis patients to anti-TNF-α therapy.
    Article Snippet: .. Immunofluorescence staining was performed in a darkroom using DAPI (catalog: G1012-100, Servicebio), LAMP3 (catalog: 12632-1-AP, Proteintech, dilution ratio of 1:100), and HLA-DPB1 (catalog: bs-1198R, Bioss, dilution ratio of 1:100) antibodies. .. After incubation at room temperature, the sections were rinsed with PBS (glycerin:PBS = 1:1) and mounted for observation via a laser digital scan of the tissue sections (catalog: Pannorimic MIDI, 3DHISTECH) to assess the intracellular localization of the target proteins.

    Staining:

    Article Title: Single-cell RNA sequencing reveals the adverse role of cDC3s in the response of ulcerative colitis patients to anti-TNF-α therapy
    Article Snippet: .. Immunofluorescence staining was performed in a darkroom using DAPI (catalog: G1012-100, Servicebio), LAMP3 (catalog: 12632-1-AP, Proteintech, dilution ratio of 1:100), and HLA-DPB1 (catalog: bs-1198R, Bioss, dilution ratio of 1:100) antibodies. .. After incubation at room temperature, the sections were rinsed with PBS (glycerin:PBS = 1:1) and mounted for observation via a laser digital scan of the tissue sections (catalog: Pannorimic MIDI, 3DHISTECH) to assess the intracellular localization of the target proteins.

    Article Title: Single-cell RNA sequencing reveals the adverse role of cDC3s in the response of ulcerative colitis patients to anti-TNF-α therapy.
    Article Snippet: .. Immunofluorescence staining was performed in a darkroom using DAPI (catalog: G1012-100, Servicebio), LAMP3 (catalog: 12632-1-AP, Proteintech, dilution ratio of 1:100), and HLA-DPB1 (catalog: bs-1198R, Bioss, dilution ratio of 1:100) antibodies. .. After incubation at room temperature, the sections were rinsed with PBS (glycerin:PBS = 1:1) and mounted for observation via a laser digital scan of the tissue sections (catalog: Pannorimic MIDI, 3DHISTECH) to assess the intracellular localization of the target proteins.

    Article Title: An atlas of epithelial cell states and plasticity in lung adenocarcinoma
    Article Snippet: .. Tissue sections were obtained for H&E staining and assessment of tumour development, and unstained sections were used for IF analysis using antibodies against GFP (AB13970, Abcam, 1:5000), LAMP3 (391005, Synaptic Systems, 1:10,000), KRT8 (TROMA-I, University of Iowa Developmental Studies Hybridoma Bank, 1:100), PDPN (clone 8.1.1, University of Iowa Developmental Studies Hybridoma Bank, 1:100), claudin 4 (ZMD.306, Invitrogen, 1:250), and PRKCDBP (cavin 3, Proteintech, 1:250). ..

    other:

    Article Title: Rilmenidine attenuates intracerebral hemorrhage by regulating autophagy flux and apoptosis via the HO-1/Fe 2+ /ERK pathway.
    Article Snippet: Intracerebral hemorrhage (ICH) associated with high mortality and disability rates.. The limited understanding of the pathological mechanism of ICH has greatly limited the available treatment options.. Rilmenidine (RIL) is a safe and effective imidazoline-1 receptor agonist that has been confirmed by the FDA to have good blood pressure-reducing effects.



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    Image Search Results


    TWIST1 is required for mesothelial cell differentiation (A) Schematic of experimental procedure used to assay lung regeneration in Wt1creER;Twist1;tdTomato mutant mice and corresponding controls. (B-C) Representative immunofluorescent labeling of WT1 on top of lineage marker tdTomato in Wt1creER;Twist1;tdT mutant lungs and corresponding controls at day 21 post sham or PNX. Thickness of lineage traced cells was quantified in (C). n=5 for each group. Scale bars, 20 μm. (D) Quantification of Wt1 in Wt1creER;Twist1 mutant lungs and corresponding controls at day 7 post surgery, as assayed by RT-qPCR. n=3 for each group. (E-F) Representative images of all right lobes at day 21 post surgery in Wt1creER;Twist1 mutant lungs and littermate controls. Quantification of dry weight was shown in (F). n=3 for each group. Scale bars, 0.5 cm. (G-H) Representative immunofluorescent labeling of EdU signals on top of LAMP3 in the lung of Wt1creER;Twist1;tdT mutants and relative controls at 7 days post PNX. Quantifications were shown in (H). n=3 for each group. Scale bars, 20 μm. (I-J) Flow cytometry analysis of F4/80+CD11b+ monocytes and their derived cells as gated in live CD45+ singlet in the lung of Wt1creER;Twist1;tdT mutants and littermate controls at 7 days post surgery. Quantifications within littermates were shown in (J). n=3 for each group. (K-L) Representative immunofluorescent labeling of CD11B with tdTomato reporter in the lung of Wt1creER;Twist1;tdT mutants and littermate controls at day 7 post surgery. Quantifications were shown in (L). n=4 for each group. Scale bars, 20 μm.

    Journal: Research Square

    Article Title: Mesothelial Plasticity Specifies Divergent Pleural Immune Circuits that Instruct Lung Regeneration versus Degeneration

    doi: 10.21203/rs.3.rs-10472664/v1

    Figure Lengend Snippet: TWIST1 is required for mesothelial cell differentiation (A) Schematic of experimental procedure used to assay lung regeneration in Wt1creER;Twist1;tdTomato mutant mice and corresponding controls. (B-C) Representative immunofluorescent labeling of WT1 on top of lineage marker tdTomato in Wt1creER;Twist1;tdT mutant lungs and corresponding controls at day 21 post sham or PNX. Thickness of lineage traced cells was quantified in (C). n=5 for each group. Scale bars, 20 μm. (D) Quantification of Wt1 in Wt1creER;Twist1 mutant lungs and corresponding controls at day 7 post surgery, as assayed by RT-qPCR. n=3 for each group. (E-F) Representative images of all right lobes at day 21 post surgery in Wt1creER;Twist1 mutant lungs and littermate controls. Quantification of dry weight was shown in (F). n=3 for each group. Scale bars, 0.5 cm. (G-H) Representative immunofluorescent labeling of EdU signals on top of LAMP3 in the lung of Wt1creER;Twist1;tdT mutants and relative controls at 7 days post PNX. Quantifications were shown in (H). n=3 for each group. Scale bars, 20 μm. (I-J) Flow cytometry analysis of F4/80+CD11b+ monocytes and their derived cells as gated in live CD45+ singlet in the lung of Wt1creER;Twist1;tdT mutants and littermate controls at 7 days post surgery. Quantifications within littermates were shown in (J). n=3 for each group. (K-L) Representative immunofluorescent labeling of CD11B with tdTomato reporter in the lung of Wt1creER;Twist1;tdT mutants and littermate controls at day 7 post surgery. Quantifications were shown in (L). n=4 for each group. Scale bars, 20 μm.

    Article Snippet: Primary antibodies with final concentrations used for immunofluorescence staining are: rabbit anti-Wilms Tumor 1 (WT1) monoclonal antibody [10 mg/ml] (ab89901, Abcam), rabbit anti-TWIST1 polyclonal antibody [10 mg/ml] (25465-1-AP, Proteintech), rabbit anti-MKI67 monoclonal antibody [5 mg/ml] (ab15580, Abcam), rabbit anti-N-Cadherin monoclonal antibody [5 mg/ml] (MA5-46719, Invitrogen), Guinea Pig anti-LAMP3 polyclonal antibody [5 mg/ml] (391005, Synaptic Systems), mouse anti-ACTA2 monoclonal antibody [5 mg/ml] (A5228, Sigma) and rat anti-CD11b monoclonal antibody [10 mg/ml] (20-0112, Tonbo).

    Techniques: Cell Differentiation, Mutagenesis, Labeling, Marker, Quantitative RT-PCR, Flow Cytometry, Derivative Assay