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2 amino 4 phosphonobutyric acid 10 µm l ap4 23052 81 5 tocris uk  (Tocris)


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    Tocris 2 amino 4 phosphonobutyric acid 10 µm l ap4 23052 81 5 tocris uk
    2 Amino 4 Phosphonobutyric Acid 10 µm L Ap4 23052 81 5 Tocris Uk, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 695 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 amino 4 phosphonobutyric acid 10 µm l ap4 23052 81 5 tocris uk/product/Tocris
    Average 95 stars, based on 695 article reviews
    2 amino 4 phosphonobutyric acid 10 µm l ap4 23052 81 5 tocris uk - by Bioz Stars, 2026-02
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    (A) Current-clamp recording from an AII-AC after 1 second current steps before (i) and after (ii) the application of a synaptic blocker cocktail containing the AMPAR blocker CNQX (10µM), the NMDAR blocker DL-AP5 (50µM), the GABA(A)R blocker gabazine (8µM) and the group-III mGluR agonist <t>L-AP4</t> (10µM). (B) Voltage traces at several current injections show dramatic differences in AII-AC Vm and spiking before (i) and after (ii) the cocktail. Current step injection values are shown in red. (C) Quantification of resting membrane voltage (i) , input resistance at −55 mV (ii) , and IV curve (iii) in control ( black ) and cocktail ( blue ). n = 7. Circles in bar graphs represent single cells. Statistical significance was determined using paired t -tests; **: p < 0.01.
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    (A) Current-clamp recording from an AII-AC after 1 second current steps before (i) and after (ii) the application of a synaptic blocker cocktail containing the AMPAR blocker CNQX (10µM), the NMDAR blocker DL-AP5 (50µM), the GABA(A)R blocker gabazine (8µM) and the group-III mGluR agonist L-AP4 (10µM). (B) Voltage traces at several current injections show dramatic differences in AII-AC Vm and spiking before (i) and after (ii) the cocktail. Current step injection values are shown in red. (C) Quantification of resting membrane voltage (i) , input resistance at −55 mV (ii) , and IV curve (iii) in control ( black ) and cocktail ( blue ). n = 7. Circles in bar graphs represent single cells. Statistical significance was determined using paired t -tests; **: p < 0.01.

    Journal: bioRxiv

    Article Title: Dopamine regulates the membrane potential and glycine release of AII amacrine cells via D1-like receptor modulation of gap junction coupling

    doi: 10.1101/2024.12.11.625486

    Figure Lengend Snippet: (A) Current-clamp recording from an AII-AC after 1 second current steps before (i) and after (ii) the application of a synaptic blocker cocktail containing the AMPAR blocker CNQX (10µM), the NMDAR blocker DL-AP5 (50µM), the GABA(A)R blocker gabazine (8µM) and the group-III mGluR agonist L-AP4 (10µM). (B) Voltage traces at several current injections show dramatic differences in AII-AC Vm and spiking before (i) and after (ii) the cocktail. Current step injection values are shown in red. (C) Quantification of resting membrane voltage (i) , input resistance at −55 mV (ii) , and IV curve (iii) in control ( black ) and cocktail ( blue ). n = 7. Circles in bar graphs represent single cells. Statistical significance was determined using paired t -tests; **: p < 0.01.

    Article Snippet: Drugs and salts stock solutions were prepared with ultra-pure water (Mili-Q, Millipore) and stored in freezer at −20° C. Synaptic blockers CNQX, DL-AP5, gabazine (SR95531) and L-AP4, were all purchased from Tocris Bioscience.

    Techniques: Injection, Membrane, Control