klc1  (Bioss)

Journal: Translational Lung Cancer Research

Article Title: Endoplasmic reticulum stress-related genes contribute to lung cancer risk: a multiomics data integration study

doi: 10.21037/tlcr-2025-474

Figure Lengend Snippet: Integrated multiomics analysis of KLC1 . (A) SMR analysis results from the integration of lung cancer GWAS and mQTL data at the KLC1 locus. (B) SMR analysis results of lung cancer GWAS and eQTL at the KLC1 locus. (C) SMR analysis results of lung cancer GWAS and pQTL at the KLC1 locus. (D) Correlation of SNP effect sizes for KLC1 between lung cancer GWAS and QTL data (mQTL, eQTL, pQTL). cis-QTL, cis-acting quantitative trait loci; eQTL, expression quantitative trait loci; GWAS, genome-wide association study; mQTL, methylation quantitative trait loci; pQTL, protein quantitative trait loci; SMR, summary data-based Mendelian randomization; SNP, single-nucleotide polymorphism.

Article Snippet: The antibodies employed in this IHC analysis were BCL2L1 (bs-1336R; Bioss Antibodies, Woburn, MA, USA) and KLC1 (bs-11212R; Bioss Antibodies).

Techniques: Expressing, GWAS, Methylation

Journal: Translational Lung Cancer Research

Article Title: Endoplasmic reticulum stress-related genes contribute to lung cancer risk: a multiomics data integration study

doi: 10.21037/tlcr-2025-474

Figure Lengend Snippet: Integrated multiomics analysis of BCL2L1 . (A) SMR analysis results from the integration of lung cancer GWAS and mQTL data at the BCL2L1 locus. (B) SMR analysis results of lung cancer GWAS and eQTL data at the BCL2L1 locus. (C) Correlation of SNP effect sizes for BCL2L1 between lung cancer GWAS and QTL data (mQTL, eQTL). (D) Manhattan plots displaying the SMR results for KLC1 and BCL2L1 at the multiomics level. CHR on the X-axis indicates chromosome numbers. cis-QTL, cis-acting quantitative trait loci; eQTL, expression quantitative trait loci; GWAS, genome-wide association study; mQTL, methylation quantitative trait loci; pQTL, protein quantitative trait loci; SNP, single-nucleotide polymorphism; SMR, summary data-based Mendelian randomization.

Article Snippet: The antibodies employed in this IHC analysis were BCL2L1 (bs-1336R; Bioss Antibodies, Woburn, MA, USA) and KLC1 (bs-11212R; Bioss Antibodies).

Techniques: Expressing, GWAS, Methylation

Journal: Translational Lung Cancer Research

Article Title: Endoplasmic reticulum stress-related genes contribute to lung cancer risk: a multiomics data integration study

doi: 10.21037/tlcr-2025-474

Figure Lengend Snippet: Expression and prognostic analysis of BCL2L1 and KCL1 . (A) Differential expression analysis of KLC1 and BCL2L1 between lung cancer and normal samples. (B) Comparison of immune cell infiltration levels in the lung cancer microenvironment between high- and low-expression groups for KLC1 and BCL2L1. (C) Overall survival analysis comparing high- and low-expression groups of KLC1 and BCL2L1. (D) Immunohistochemical analyses of KLC1 in lung cancer tissue from the HPA database. ( https://www.proteinatlas.org/ENSG00000126214-KLC1/cancer/lung+cancer ). (E) Immunohistochemical staining of BCL2L1 and KLC1 in lung cancer and normal lung tissues (scale bar: 50 µm). *, P<0.05; **, P<0.01; ***, P<0.001. CI, confidence interval; HPA, Human Protein Atlas; HR, hazard ratio; CI, confident interval; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; N, normal lung tissues; T, lung cancer tissues; TPM, transcripts per million.

Article Snippet: The antibodies employed in this IHC analysis were BCL2L1 (bs-1336R; Bioss Antibodies, Woburn, MA, USA) and KLC1 (bs-11212R; Bioss Antibodies).

Techniques: Expressing, Quantitative Proteomics, Comparison, Immunohistochemical staining, Staining

Journal: Translational Lung Cancer Research

Article Title: Endoplasmic reticulum stress-related genes contribute to lung cancer risk: a multiomics data integration study

doi: 10.21037/tlcr-2025-474

Figure Lengend Snippet: Diagram illustrating the regulatory mechanisms and functions of the ERS-related genes KLC1 and BCL2L1 in lung cancer. ERS, endoplasmic reticulum stress.

Article Snippet: The antibodies employed in this IHC analysis were BCL2L1 (bs-1336R; Bioss Antibodies, Woburn, MA, USA) and KLC1 (bs-11212R; Bioss Antibodies).

Techniques:

Journal: British Journal of Cancer

Article Title: Altered expression of vesicular trafficking machinery in prostate cancer affects lysosomal dynamics and provides insight into the underlying biology and disease progression

doi: 10.1038/s41416-024-02829-x

Figure Lengend Snippet: Antibody reagents

Article Snippet: Hs00194316_m1 , KLC1.

Techniques: Western Blot, Immunohistochemistry-IF

Journal: British Journal of Cancer

Article Title: Altered expression of vesicular trafficking machinery in prostate cancer affects lysosomal dynamics and provides insight into the underlying biology and disease progression

doi: 10.1038/s41416-024-02829-x

Figure Lengend Snippet: TaqMan assays

Article Snippet: Hs00194316_m1 , KLC1.

Techniques:

Journal: British Journal of Cancer

Article Title: Altered expression of vesicular trafficking machinery in prostate cancer affects lysosomal dynamics and provides insight into the underlying biology and disease progression

doi: 10.1038/s41416-024-02829-x

Figure Lengend Snippet: SiRNA reagents

Article Snippet: Hs00194316_m1 , KLC1.

Techniques:

Journal: British Journal of Cancer

Article Title: Altered expression of vesicular trafficking machinery in prostate cancer affects lysosomal dynamics and provides insight into the underlying biology and disease progression

doi: 10.1038/s41416-024-02829-x

Figure Lengend Snippet: Plasmid constructs

Article Snippet: Hs00194316_m1 , KLC1.

Techniques: Plasmid Preparation

Journal: Clinics (Sao Paulo, Brazil)

Article Title: The supramolecular polymer-related signature predicts prognosis and indicates immune microenvironment infiltration in gastric cancer.

doi: 10.1016/j.clinsp.2025.100641

Figure Lengend Snippet: Fig. 10. Biological function of KLC1 in GC cells. (A) Comparison of the mRNA level of KLC1 between the MKN45 and normal cell. (B) Comparison of the relative mRNA.level between the control and siKLC1 group. (C) Western blot analysis showing the protein expression levels of KLC1 and GAPDH (loading control) in control and siKLC1 groups. The protein level of KLC1 is reduced in the siKLC1 group. (D) Representative images of crystal violet-stained cell invasion assays for control and siKLC1 groups. Cells were stained blue, indicating invasive capacity. (E) Quantification of relative invasion rates between control and siKLC1 groups. The invasion rate is significantly lower in the siKLC1 group. (F) Colony formation assay showing the number of colonies formed by control and siKLC1 groups. Fewer colonies are observed in the siKLC1 group. (G) Quantification of relative colony numbers between control and siKLC1 groups. The number of colonies is significantly reduced in the siKLC1 group. (H) Wound healing assay images at 0 h and 24 h post-wounding for control and siKLC1 groups. The wound closure is slower in the siKLC1 group. (I) Quantification of relative wound closure between control and siKLC1 groups. The wound closure rate is significantly lower in the siKLC1 group. ** p < 0.01.

Article Snippet: Membranes were blocked with 5 % non-fat milk in TBST for 1 h at room temperature and then incubated overnight at 4 ◦C with primary antibodies against KLC1 (Santa Cruz Biotechnology, 1:1000 dilution) and GAPDH (Cell Signaling Technology, 1:5000 dilution).

Techniques: Comparison, Control, Western Blot, Expressing, Staining, Colony Assay, Wound Healing Assay