kcns1  (Bioss)

Journal: Frontiers in Neurology

Article Title: Identification of key potassium channel genes of temporal lobe epilepsy by bioinformatics analyses and experimental verification

doi: 10.3389/fneur.2023.1175007

Figure Lengend Snippet: Primer sequences for quantitative real-time polymerase chain reaction (qRT-PCR).

Article Snippet: Primary antibodies against mouse monoclonal KCNA1 (bs-8691R), KCNA2 (bs- 12182R), KCNJ11 (bs-2436R), and KCNS1 (bs- 16916R) (1:1000) were purchased from BIOSS (Beijing, China).

Techniques: Real-time Polymerase Chain Reaction

Journal: Frontiers in Neurology

Article Title: Identification of key potassium channel genes of temporal lobe epilepsy by bioinformatics analyses and experimental verification

doi: 10.3389/fneur.2023.1175007

Figure Lengend Snippet: Histone modification sites of proteins encoded by each TERKPCG.

Article Snippet: Primary antibodies against mouse monoclonal KCNA1 (bs-8691R), KCNA2 (bs- 12182R), KCNJ11 (bs-2436R), and KCNS1 (bs- 16916R) (1:1000) were purchased from BIOSS (Beijing, China).

Techniques: Modification, Sequencing

Journal: Journal of Neurophysiology

Article Title: Molecular determinants of afferent sensitization in a rat model of cystitis with urothelial barrier dysfunction

doi: 10.1152/jn.00306.2019

Figure Lengend Snippet: TaqMan probes for real-time quantitative PCR

Article Snippet: Copy number for each amplicon was normalized to the value obtained with the internal control, β-actin. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Protein Probe Fluorophore Actb Actin Rn00667869_m1 VIC Kcna1 K v 1.1 Rn00597355_s1 FAM Kcnb1 K v 2.1 Rn00755102_m1 FAM Kcnb2 K v 2.2 Rn00588883_m1 FAM Kcnc4 K v 3.4 Rn01748431_m1 FAM Kcnd1 K v 4.1 Rn01525167_m1 FAM Kcnq2 K v 7.2 Rn00591249_m1 FAM Kcnq3 K v 7.3 Rn00580995_m1 FAM Kcns1 K v 9.1 Rn00588597_m1 FAM Kcnma1 BKCa, maxiK Rn00582881_m1 FAM Na v 1.3 Scn3a Rn01485332_m1 FAM Na v 1.6 Scn8a Rn00570506_m1 FAM Na v 1.7 Scn9a Rn00591020_m1 FAM Open in a separate window TaqMan probes for real-time quantitative PCR

Techniques:

Journal: Journal of Neurophysiology

Article Title: Molecular determinants of afferent sensitization in a rat model of cystitis with urothelial barrier dysfunction

doi: 10.1152/jn.00306.2019

Figure Lengend Snippet: Voltage-gated K+ channel (Kv)9.1 alters the voltage dependence of activation of Kv2.2 channels. Xenopus oocytes were injected with cRNAs encoding for Kv2.2 or Kv2.2 and Kv9.1 at a 1-to-1 or 1-to-4 ratio. Two-electrode voltage clamp was performed as described in materials and methods. A: representative recording showing the voltage dependence of activation of Kv2.2. Currents were measured 24 h after injection in an oocyte injected with Kv2.2 and Kv9.1 (ratio 1:1). The protocol used to study voltage dependence of activation is shown at top. Outward currents were evoked by 1-s depolarizing voltage steps from −70 to +70 mV in 10-mV increments after the application of a 3-s prepulse to −90 mV to remove inactivation. B: normalized conductance (G/Gmax)-voltage relationships for Kv2.2 (n = 30), Kv2.2-Kv9.1 (ratio 1:1) (n = 27), and Kv2.2-Kv9.1 (ratio 1:4 (n = 18). Note that the coexpression of Kv9.1 with Kv2.2 at 1:1 and 1:4 results in a significant change in the voltage of half-maximal activation (P < 0.05, n = 18–30). Data were fitted to a Boltzmann function as described in materials and methods. C: representative recording showing the voltage dependence of inactivation of Kv2.2. Currents were measured 24 h after injection in an oocyte injected with Kv2.2 and Kv9.1 (ratio 1:4). The protocol used to study voltage dependence of inactivation for Kv2.2 is shown at top. Currents were evoked at a membrane potential of +60 mV after a prepulse of 10 s from −80 to 20 mV. D: normalized current (I/I−80 mV)-voltage relationships for Kv2.2 (n = 20), Kv2.2-Kv9.1 (ratio 1:1) (n = 21), and Kv2.2-Kv9.1 (ratio 1:4) (n = 18). Data were fitted to a Boltzmann function as described in materials and methods. Note that the coexpression of Kv9.1 and Kv2.2 does not alter the voltage dependence of inactivation.

Article Snippet: Copy number for each amplicon was normalized to the value obtained with the internal control, β-actin. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Protein Probe Fluorophore Actb Actin Rn00667869_m1 VIC Kcna1 K v 1.1 Rn00597355_s1 FAM Kcnb1 K v 2.1 Rn00755102_m1 FAM Kcnb2 K v 2.2 Rn00588883_m1 FAM Kcnc4 K v 3.4 Rn01748431_m1 FAM Kcnd1 K v 4.1 Rn01525167_m1 FAM Kcnq2 K v 7.2 Rn00591249_m1 FAM Kcnq3 K v 7.3 Rn00580995_m1 FAM Kcns1 K v 9.1 Rn00588597_m1 FAM Kcnma1 BKCa, maxiK Rn00582881_m1 FAM Na v 1.3 Scn3a Rn01485332_m1 FAM Na v 1.6 Scn8a Rn00570506_m1 FAM Na v 1.7 Scn9a Rn00591020_m1 FAM Open in a separate window TaqMan probes for real-time quantitative PCR

Techniques: Activation Assay, Injection, Membrane