Journal: Journal of molecular histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway.

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Fig. 1 Effects of J147 on the viability of BV2 cells. a Chemical structure of J147. b BV2 cells were treated with J147 (0 and 25 μM). After 26 h, cell viability was assessed using CCK-8 (n = 8 per group). c Activation of BV2 cells was visualized by immunostaining with Iba1 antibody following J147 treatment in the presence of LPS (1 μg/mL) for 26 h. d Mean fluorescence intensity of Iba1 (n = 20 per group) (one way ANOVA, F(3,76) = 15.27, p < 0.0001). Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test. **p < 0.01, ***p < 0.001; ****p < 0.0001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: CCK-8 Assay, Activation Assay, Immunostaining, Fluorescence

Journal: Journal of molecular histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway.

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Fig. 2 Effect of J147 on expression of proinflammatory cytokines in LPS-induced BV2 cells. BV2 cells were pretreated with J147 (2.5 μM) for 2 h before incubation with LPS (1 μg/mL) for 24 h. a–d Effect of J147 on mRNA expression of IL-6 (one way ANOVA, F(3,20) = 3.539, p = 0.0333), IL-1β (one way ANOVA, F(3,20) = 9.469, p = 0.0004), and TNF-α (one way ANOVA, F(3,20) = 5.557, p = 0.0061) in BV2 cells. e Protein expression of IL-6 (one way ANOVA, F(3,20) = 59.12, p < 0.0001) and TNF-α (one way ANOVA, F(3,20) = 46.46, p < 0.0001) was determined using ELISA kits. Columns present the mean ± SEM (n = 6 per group). ANOVA followed by Bonferroni post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Expressing, Incubation, Enzyme-linked Immunosorbent Assay

Journal: Journal of molecular histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway.

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Fig. 4 Effect of J147 on the nuclear translocation of NF-κB p65. a BV2 cells were pretreated with J147 (2.5 μM) for 2 h before treatment with LPS (1 μg/mL) for 24 h. Localization of NF-κB p65 was visualized by confocal laser scanning microscopy following immunofluorescence staining with an anti-NF-κB antibody (red) combined with DAPI for nuclei (blue). Results are representative of four groups. b Quantification of the data shown in a (n = 14 per group) (one way ANOVA, F(3,52) = 4.92, p = 0.0044). Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test. *p < 0.05, ***p < 0.001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Translocation Assay, Confocal Laser Scanning Microscopy, Immunofluorescence, Staining

Journal: Journal of molecular histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway.

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Fig. 5 Effects of J147 on depressive-like behaviors in LPS-induced mice. a Experimental protocol. b J147 treatment prevented the bodyweight loss induced by LPS. Weight loss of mice was expressed as mean ± SEM (n = 8 mice for each group) (two-way ANOVA followed by Turkey post hoc analyses, Time × LPS: F (16, 168) = 3.009, p = 0.002; Time: F (1.563, 32.82) = 16.14, p < 0.0001; LPS: F (2, 21) = 7.839, p = 0.0029), Ctrl vs LPS, *p < 0.05, **p < 0.01; LPS vs J147 + LPS #p < 0.05, ##p < 0.05. c. Survival was monitored every day after LPS injection. (Ctrl, n = 8 mice; LPS, n = 21 mice; LPS + J147 mice, n = 12 mice). d Performance of rotarod test among three group. (n = 8 mice for each group). (p > 0.05). e The total distance travelled in OFT test among three groups (one way ANOVA, F(2,21) = 1.898, p = 0.1747). (n = 8 mice for each group). f Performance of TST among three groups. (n = 8 mice for each group) (one way ANOVA, F(2,21) = 5.960, p = 0.0089). g Performance of FST among three groups. (n = 8 micefor each group) (one way ANOVA, F(2,21) = 4.985, p = 0.0169). Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Injection