Journal: Journal of Molecular Histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Effects of J147 on the viability of BV2 cells. a Chemical structure of J147. b BV2 cells were treated with J147 (0 and 25 μM). After 26 h, cell viability was assessed using CCK-8 (n = 8 per group). c Activation of BV2 cells was visualized by immunostaining with Iba1 antibody following J147 treatment in the presence of LPS (1 μg/mL) for 26 h. d Mean fluorescence intensity of Iba1 (n = 20 per group) (one way ANOVA, F (3 , 76) = 15.27, p < 0.0001). Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test. ** p < 0.01, *** p < 0.001; **** p < 0.0001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: CCK-8 Assay, Activation Assay, Immunostaining, Fluorescence

Journal: Journal of Molecular Histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Effect of J147 on expression of proinflammatory cytokines in LPS-induced BV2 cells. BV2 cells were pretreated with J147 (2.5 μM) for 2 h before incubation with LPS (1 μg/mL) for 24 h. a – d Effect of J147 on mRNA expression of IL-6 (one way ANOVA, F (3 , 20) = 3.539, p = 0.0333), IL-1β (one way ANOVA, F (3 , 20) = 9.469, p = 0.0004), and TNF-α (one way ANOVA, F (3 , 20) = 5.557, p = 0.0061) in BV2 cells. e Protein expression of IL-6 (one way ANOVA, F (3 , 20) = 59.12, p < 0.0001) and TNF-α (one way ANOVA, F (3 , 20) = 46.46, p < 0.0001) was determined using ELISA kits. Columns present the mean ± SEM (n = 6 per group). ANOVA followed by Bonferroni post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Expressing, Incubation, Enzyme-linked Immunosorbent Assay

Journal: Journal of Molecular Histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Effect of J147 on the TLR4 and NF-κB signaling pathways in LPS-induced BV2 cells. a Protein expression of TLR4 (one way ANOVA, F (3 , 20) = 19.59, p < 0.0001), p-IκBα (one way ANOVA, F (3 , 20) = 8.128, p = 0.0010), and p-NF-κB p-p65 (one way ANOVA, F (3 , 20) = 4.347, p = 0.0164) in whole cells (n = 6 per group), IκBα (one way ANOVA, F (3 , 12) = 3.701, p = 0.0428) in the cytoplasm and NF-κB p65 (one way ANOVA, F (3 , 12) = 21.73, p < 0.0001) in the nucleus in J147-treated BV2 microglia cells (n = 4 per group). b – f Quantitative analysis of the Western blots. Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, *** p < 0.0001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Protein-Protein interactions, Expressing, Western Blot

Journal: Journal of Molecular Histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Effect of J147 on the nuclear translocation of NF-κB p65. a BV2 cells were pretreated with J147 (2.5 μM) for 2 h before treatment with LPS (1 μg/mL) for 24 h. Localization of NF-κB p65 was visualized by confocal laser scanning microscopy following immunofluorescence staining with an anti-NF-κB antibody (red) combined with DAPI for nuclei (blue). Results are representative of four groups. b Quantification of the data shown in a (n = 14 per group) (one way ANOVA, F (3 , 52) = 4.92, p = 0.0044). Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test. * p < 0.05, *** p < 0.001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Translocation Assay, Confocal Laser Scanning Microscopy, Immunofluorescence, Staining

Journal: Journal of Molecular Histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Effects of J147 on depressive-like behaviors in LPS-induced mice. a Experimental protocol. b J147 treatment prevented the bodyweight loss induced by LPS. Weight loss of mice was expressed as mean ± SEM (n = 8 mice for each group) (two-way ANOVA followed by Turkey post hoc analyses, Time × LPS: F (16, 168) = 3.009, p = 0.002; Time: F (1.563, 32.82) = 16.14, p < 0.0001; LPS: F (2, 21) = 7.839, p = 0.0029), Ctrl vs LPS, * p < 0.05, ** p < 0.01; LPS vs J147 + LPS # p < 0.05, ## p < 0.05. c . Survival was monitored every day after LPS injection. (Ctrl, n = 8 mice; LPS, n = 21 mice; LPS + J147 mice, n = 12 mice). d Performance of rotarod test among three group. (n = 8 mice for each group). ( p > 0.05). e The total distance travelled in OFT test among three groups (one way ANOVA, F (2 , 21) = 1.898, p = 0.1747). (n = 8 mice for each group). f Performance of TST among three groups. (n = 8 mice for each group) (one way ANOVA, F (2 , 21) = 5.960, p = 0.0089). g Performance of FST among three groups. (n = 8 micefor each group) (one way ANOVA, F (2 , 21) = 4.985, p = 0.0169). Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Injection

Journal: Journal of Molecular Histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: J147 decreased LPS-induced microglia activation in the hippocampus. a Immunofluorescence staining of Iba-1 in the CA1 hippocampal area. Scale bar , 20 μm. b Quantification of Iba1-positive cells in microglia density. (n = 41 brain slice from 3 mice per group) (one way ANOVA, F (2 , 120) = 27.3, p < 0.0001). c Quantification of microglia in the soma. (n = 96 cell from 3 mice per group) (one way ANOVA, F (2 , 285) = 50.69, p < 0.0001). Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test. **** p < 0.0001

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Activation Assay, Immunofluorescence, Staining, Slice Preparation

Journal: Journal of Molecular Histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Effect of J147 on LPS-induced neuroinflammatory cytokine levels in mouse hippocampus. a – c mRNA expression of TNF-a (one way ANOVA, F (2 , 13) = 19.88, p = 0.0001), IL-1β (one way ANOVA, F (2 , 13) = 7.816, p = 0.0059) and IL-6 (one way ANOVA, F (2 , 13) = 38.69, p < 0.0001) measured by RT -q PCR (Ctrl, n = 6 mice; LPS, n = 6 mice; LPS + J147, n = 4 mice). d Representative Western blot. e , f Quantitative analysis of d in the hippocampus among the three groups (n = 3 mice per group), TLR-4 (one way ANOVA, F (2 , 6) = 21.28, p = 0.0019), and NF-κB p65 (one way ANOVA, F (2 , 6) = 10.97, p = 0.0099). Data are the mean ± SEM. ANOVA followed by Bonferroni post hoc test.* p < 0.05, ** p < 0.01

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques: Expressing, Western Blot

Journal: Journal of Molecular Histology

Article Title: J147 ameliorates sepsis-induced depressive-like behaviors in mice by attenuating neuroinflammation through regulating the TLR4/NF-κB signaling pathway

doi: 10.1007/s10735-023-10147-4

Figure Lengend Snippet: Potential mechanism of anti-depressive effects of J147

Article Snippet: J147 was purchased from Selleck Chemicals (catalog number: S5269; Houston, TX, USA) and dissolved in 2% dimethyl sulfoxide and phosphate-buffered saline (PBS: 0.01 M) to make a final concentration of 2.5 μM.

Techniques:

Journal: Alzheimer's & Dementia : Translational Research & Clinical Interventions

Article Title: Alzheimer's disease drug development pipeline: 2019

doi: 10.1016/j.trci.2019.05.008

Figure Lengend Snippet: Agents currently in phase 1 of Alzheimer's disease drug development (as of February 12, 2019)

Article Snippet: J147 , Neuroprotective , Mitochondrial ATP synthase inhibitor , Protects neurons from multiple toxicities associated with aging (DMT) , NCT03838185 , Recruiting , Abrexa , Jan 2019 , Jan 2020.

Techniques: Virus, Plasmid Preparation, Expressing, Activity Assay, Reverse Transcription, Western Blot, Histone Deacetylase Assay