Journal: Nature Immunology
Article Title: A distinct monocyte transcriptional state links systemic immune dysregulation to pulmonary impairment in long COVID
doi: 10.1038/s41590-025-02387-1
Figure Lengend Snippet: a , Gating strategy implemented to identify Monocyte subsets in cohort 4. b , Histograms for the flow cytometry analysis of CALR, CD99, TGFB1, HLA-DQ, and CD120b surface expression enriched for all CD14 + monocytes from R A +NI compared to LC. R A +NI, LC antibody, and isotype-stained cells are shown in blue, red, and black, respectively. Shown is a representative donor of biological replicates with similar results c , Comparison of Median Fluorescence Intensity (MFI) of measured markers (IFNGR1, CD51, CD105, IRF8, HLA-DR, CD16 and CD163) in CD14 + monocytes. R A +NI (n = 10), LC AM (n = 29), LC AS (n = 11). P-values calculated using two-sided Wilcox Rank Sum Test * **: p value < 0.00001, ***: p value < 0.001, **: p value < 0.01, *: p value < 0.05. Boxplot shows the median (centre), first and third quartiles(bounds) and 1.5 times the interquartile range (whiskers) d , TGFB1 MFI correlation with blood pO2 (top), the gray shaded area indicates the 95% confidence interval. correlation of significant markers in LC patients among each other (bottom). Spearman correlation P values were computed using a t-distribution approximation applied to rank-transformed data. ***: p value < 0.001, **: p value < 0.01, *: p value < 0.05.
Article Snippet: IRF8 , PE , REA516 , Miltenyi , 130-122-927.
Techniques: Flow Cytometry, Expressing, Staining, Comparison, Fluorescence, Transformation Assay