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anti iqgap3  (Proteintech)


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    Proteintech anti iqgap3
    <t>IQGAP3</t> reprogram macrophages through FYN-p-STAT1 axis. A Volcano plot showing differentially expressed genes revealed by RNA-seq analyses comparing sh-FOXM1 THP-1 cells and sh-NC THP-1 cells. Significantly upregulated (red) and downregulated (blue) genes are indicated, with thresholds set at |log₂ fold change|> 1 and adjusted p -value < 0.05. B Gene Set Enrichment Analysis (GSEA) of RNA-seq data revealed that a significant increase in the enrichment of the response to type I interferon, regulation of inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein in the IQGAP3 knockdown group. C The protein expression of p-STAT1 (Tyr701) and STAT1 were examined by WB in IQGAP3-knockdown THP-1 or BMDMs. D Representative histogram plot of flow cytometry and proportions of M1 macrophages (CD86 +) and M2 macrophages (CD206 +) in each indicated group. E The protein expression of FYN was examined by WB in sh-NC and sh-IQGAP3 THP-1-derived macrophages. F The protein expression of p-STAT1(Tyr701) in THP-1-derived macrophages for indicated conditions. G mRNA half-life (t 1/2 ) of FYN transcripts in sh-NC or sh-IQGAP3 THP-1-derived macrophages. H The number of the predicted binding sites for RNA binding proteins (RBPs) recorded on FYN mRNA 3′UTR region. I qPCR analysis of FYN mRNA levels in RIP assays with IgG and anti-IGFBP2 antibody in THP-1 cells. Relative enrichment is expressed by normalizing values to average IgG in each group. J mRNA half-life (t 1/2 ) of FYN transcripts in indicated groups. K The protein expression of p-STAT1(Tyr701) and FYN in THP-1 cells for indicated conditions. L Protein interaction was analyzed by co-immunoprecipitation (Co-IP) using <t>an</t> <t>anti-IQGAP3</t> antibody in THP-1 cell lysates, followed by immunoblotting for the indicated proteins. M Immunofluorescence (IF) staining (original magnification: × 40; scale bar: 25 μm) examined IQGAP3 and IGF2BP2 expression in THP-1 cells
    Anti Iqgap3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti iqgap3/product/Proteintech
    Average 93 stars, based on 11 article reviews
    anti iqgap3 - by Bioz Stars, 2026-02
    93/100 stars

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    1) Product Images from "Targeting IQGAP3 skews macrophages polarization towards M1 phenotype and promotes antitumor immune response"

    Article Title: Targeting IQGAP3 skews macrophages polarization towards M1 phenotype and promotes antitumor immune response

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-025-06044-6

    IQGAP3 reprogram macrophages through FYN-p-STAT1 axis. A Volcano plot showing differentially expressed genes revealed by RNA-seq analyses comparing sh-FOXM1 THP-1 cells and sh-NC THP-1 cells. Significantly upregulated (red) and downregulated (blue) genes are indicated, with thresholds set at |log₂ fold change|> 1 and adjusted p -value < 0.05. B Gene Set Enrichment Analysis (GSEA) of RNA-seq data revealed that a significant increase in the enrichment of the response to type I interferon, regulation of inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein in the IQGAP3 knockdown group. C The protein expression of p-STAT1 (Tyr701) and STAT1 were examined by WB in IQGAP3-knockdown THP-1 or BMDMs. D Representative histogram plot of flow cytometry and proportions of M1 macrophages (CD86 +) and M2 macrophages (CD206 +) in each indicated group. E The protein expression of FYN was examined by WB in sh-NC and sh-IQGAP3 THP-1-derived macrophages. F The protein expression of p-STAT1(Tyr701) in THP-1-derived macrophages for indicated conditions. G mRNA half-life (t 1/2 ) of FYN transcripts in sh-NC or sh-IQGAP3 THP-1-derived macrophages. H The number of the predicted binding sites for RNA binding proteins (RBPs) recorded on FYN mRNA 3′UTR region. I qPCR analysis of FYN mRNA levels in RIP assays with IgG and anti-IGFBP2 antibody in THP-1 cells. Relative enrichment is expressed by normalizing values to average IgG in each group. J mRNA half-life (t 1/2 ) of FYN transcripts in indicated groups. K The protein expression of p-STAT1(Tyr701) and FYN in THP-1 cells for indicated conditions. L Protein interaction was analyzed by co-immunoprecipitation (Co-IP) using an anti-IQGAP3 antibody in THP-1 cell lysates, followed by immunoblotting for the indicated proteins. M Immunofluorescence (IF) staining (original magnification: × 40; scale bar: 25 μm) examined IQGAP3 and IGF2BP2 expression in THP-1 cells
    Figure Legend Snippet: IQGAP3 reprogram macrophages through FYN-p-STAT1 axis. A Volcano plot showing differentially expressed genes revealed by RNA-seq analyses comparing sh-FOXM1 THP-1 cells and sh-NC THP-1 cells. Significantly upregulated (red) and downregulated (blue) genes are indicated, with thresholds set at |log₂ fold change|> 1 and adjusted p -value < 0.05. B Gene Set Enrichment Analysis (GSEA) of RNA-seq data revealed that a significant increase in the enrichment of the response to type I interferon, regulation of inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein in the IQGAP3 knockdown group. C The protein expression of p-STAT1 (Tyr701) and STAT1 were examined by WB in IQGAP3-knockdown THP-1 or BMDMs. D Representative histogram plot of flow cytometry and proportions of M1 macrophages (CD86 +) and M2 macrophages (CD206 +) in each indicated group. E The protein expression of FYN was examined by WB in sh-NC and sh-IQGAP3 THP-1-derived macrophages. F The protein expression of p-STAT1(Tyr701) in THP-1-derived macrophages for indicated conditions. G mRNA half-life (t 1/2 ) of FYN transcripts in sh-NC or sh-IQGAP3 THP-1-derived macrophages. H The number of the predicted binding sites for RNA binding proteins (RBPs) recorded on FYN mRNA 3′UTR region. I qPCR analysis of FYN mRNA levels in RIP assays with IgG and anti-IGFBP2 antibody in THP-1 cells. Relative enrichment is expressed by normalizing values to average IgG in each group. J mRNA half-life (t 1/2 ) of FYN transcripts in indicated groups. K The protein expression of p-STAT1(Tyr701) and FYN in THP-1 cells for indicated conditions. L Protein interaction was analyzed by co-immunoprecipitation (Co-IP) using an anti-IQGAP3 antibody in THP-1 cell lysates, followed by immunoblotting for the indicated proteins. M Immunofluorescence (IF) staining (original magnification: × 40; scale bar: 25 μm) examined IQGAP3 and IGF2BP2 expression in THP-1 cells

    Techniques Used: RNA Sequencing, Phospho-proteomics, Knockdown, Expressing, Flow Cytometry, Derivative Assay, Binding Assay, RNA Binding Assay, Immunoprecipitation, Co-Immunoprecipitation Assay, Western Blot, Immunofluorescence, Staining



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    <t>IQGAP3</t> reprogram macrophages through FYN-p-STAT1 axis. A Volcano plot showing differentially expressed genes revealed by RNA-seq analyses comparing sh-FOXM1 THP-1 cells and sh-NC THP-1 cells. Significantly upregulated (red) and downregulated (blue) genes are indicated, with thresholds set at |log₂ fold change|> 1 and adjusted p -value < 0.05. B Gene Set Enrichment Analysis (GSEA) of RNA-seq data revealed that a significant increase in the enrichment of the response to type I interferon, regulation of inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein in the IQGAP3 knockdown group. C The protein expression of p-STAT1 (Tyr701) and STAT1 were examined by WB in IQGAP3-knockdown THP-1 or BMDMs. D Representative histogram plot of flow cytometry and proportions of M1 macrophages (CD86 +) and M2 macrophages (CD206 +) in each indicated group. E The protein expression of FYN was examined by WB in sh-NC and sh-IQGAP3 THP-1-derived macrophages. F The protein expression of p-STAT1(Tyr701) in THP-1-derived macrophages for indicated conditions. G mRNA half-life (t 1/2 ) of FYN transcripts in sh-NC or sh-IQGAP3 THP-1-derived macrophages. H The number of the predicted binding sites for RNA binding proteins (RBPs) recorded on FYN mRNA 3′UTR region. I qPCR analysis of FYN mRNA levels in RIP assays with IgG and anti-IGFBP2 antibody in THP-1 cells. Relative enrichment is expressed by normalizing values to average IgG in each group. J mRNA half-life (t 1/2 ) of FYN transcripts in indicated groups. K The protein expression of p-STAT1(Tyr701) and FYN in THP-1 cells for indicated conditions. L Protein interaction was analyzed by co-immunoprecipitation (Co-IP) using <t>an</t> <t>anti-IQGAP3</t> antibody in THP-1 cell lysates, followed by immunoblotting for the indicated proteins. M Immunofluorescence (IF) staining (original magnification: × 40; scale bar: 25 μm) examined IQGAP3 and IGF2BP2 expression in THP-1 cells
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    <t>IQGAP3</t> reprogram macrophages through FYN-p-STAT1 axis. A Volcano plot showing differentially expressed genes revealed by RNA-seq analyses comparing sh-FOXM1 THP-1 cells and sh-NC THP-1 cells. Significantly upregulated (red) and downregulated (blue) genes are indicated, with thresholds set at |log₂ fold change|> 1 and adjusted p -value < 0.05. B Gene Set Enrichment Analysis (GSEA) of RNA-seq data revealed that a significant increase in the enrichment of the response to type I interferon, regulation of inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein in the IQGAP3 knockdown group. C The protein expression of p-STAT1 (Tyr701) and STAT1 were examined by WB in IQGAP3-knockdown THP-1 or BMDMs. D Representative histogram plot of flow cytometry and proportions of M1 macrophages (CD86 +) and M2 macrophages (CD206 +) in each indicated group. E The protein expression of FYN was examined by WB in sh-NC and sh-IQGAP3 THP-1-derived macrophages. F The protein expression of p-STAT1(Tyr701) in THP-1-derived macrophages for indicated conditions. G mRNA half-life (t 1/2 ) of FYN transcripts in sh-NC or sh-IQGAP3 THP-1-derived macrophages. H The number of the predicted binding sites for RNA binding proteins (RBPs) recorded on FYN mRNA 3′UTR region. I qPCR analysis of FYN mRNA levels in RIP assays with IgG and anti-IGFBP2 antibody in THP-1 cells. Relative enrichment is expressed by normalizing values to average IgG in each group. J mRNA half-life (t 1/2 ) of FYN transcripts in indicated groups. K The protein expression of p-STAT1(Tyr701) and FYN in THP-1 cells for indicated conditions. L Protein interaction was analyzed by co-immunoprecipitation (Co-IP) using <t>an</t> <t>anti-IQGAP3</t> antibody in THP-1 cell lysates, followed by immunoblotting for the indicated proteins. M Immunofluorescence (IF) staining (original magnification: × 40; scale bar: 25 μm) examined IQGAP3 and IGF2BP2 expression in THP-1 cells
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    <t>IQGAP3</t> reprogram macrophages through FYN-p-STAT1 axis. A Volcano plot showing differentially expressed genes revealed by RNA-seq analyses comparing sh-FOXM1 THP-1 cells and sh-NC THP-1 cells. Significantly upregulated (red) and downregulated (blue) genes are indicated, with thresholds set at |log₂ fold change|> 1 and adjusted p -value < 0.05. B Gene Set Enrichment Analysis (GSEA) of RNA-seq data revealed that a significant increase in the enrichment of the response to type I interferon, regulation of inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein in the IQGAP3 knockdown group. C The protein expression of p-STAT1 (Tyr701) and STAT1 were examined by WB in IQGAP3-knockdown THP-1 or BMDMs. D Representative histogram plot of flow cytometry and proportions of M1 macrophages (CD86 +) and M2 macrophages (CD206 +) in each indicated group. E The protein expression of FYN was examined by WB in sh-NC and sh-IQGAP3 THP-1-derived macrophages. F The protein expression of p-STAT1(Tyr701) in THP-1-derived macrophages for indicated conditions. G mRNA half-life (t 1/2 ) of FYN transcripts in sh-NC or sh-IQGAP3 THP-1-derived macrophages. H The number of the predicted binding sites for RNA binding proteins (RBPs) recorded on FYN mRNA 3′UTR region. I qPCR analysis of FYN mRNA levels in RIP assays with IgG and anti-IGFBP2 antibody in THP-1 cells. Relative enrichment is expressed by normalizing values to average IgG in each group. J mRNA half-life (t 1/2 ) of FYN transcripts in indicated groups. K The protein expression of p-STAT1(Tyr701) and FYN in THP-1 cells for indicated conditions. L Protein interaction was analyzed by co-immunoprecipitation (Co-IP) using <t>an</t> <t>anti-IQGAP3</t> antibody in THP-1 cell lysates, followed by immunoblotting for the indicated proteins. M Immunofluorescence (IF) staining (original magnification: × 40; scale bar: 25 μm) examined IQGAP3 and IGF2BP2 expression in THP-1 cells
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    Image Search Results


    IQGAP3 reprogram macrophages through FYN-p-STAT1 axis. A Volcano plot showing differentially expressed genes revealed by RNA-seq analyses comparing sh-FOXM1 THP-1 cells and sh-NC THP-1 cells. Significantly upregulated (red) and downregulated (blue) genes are indicated, with thresholds set at |log₂ fold change|> 1 and adjusted p -value < 0.05. B Gene Set Enrichment Analysis (GSEA) of RNA-seq data revealed that a significant increase in the enrichment of the response to type I interferon, regulation of inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein in the IQGAP3 knockdown group. C The protein expression of p-STAT1 (Tyr701) and STAT1 were examined by WB in IQGAP3-knockdown THP-1 or BMDMs. D Representative histogram plot of flow cytometry and proportions of M1 macrophages (CD86 +) and M2 macrophages (CD206 +) in each indicated group. E The protein expression of FYN was examined by WB in sh-NC and sh-IQGAP3 THP-1-derived macrophages. F The protein expression of p-STAT1(Tyr701) in THP-1-derived macrophages for indicated conditions. G mRNA half-life (t 1/2 ) of FYN transcripts in sh-NC or sh-IQGAP3 THP-1-derived macrophages. H The number of the predicted binding sites for RNA binding proteins (RBPs) recorded on FYN mRNA 3′UTR region. I qPCR analysis of FYN mRNA levels in RIP assays with IgG and anti-IGFBP2 antibody in THP-1 cells. Relative enrichment is expressed by normalizing values to average IgG in each group. J mRNA half-life (t 1/2 ) of FYN transcripts in indicated groups. K The protein expression of p-STAT1(Tyr701) and FYN in THP-1 cells for indicated conditions. L Protein interaction was analyzed by co-immunoprecipitation (Co-IP) using an anti-IQGAP3 antibody in THP-1 cell lysates, followed by immunoblotting for the indicated proteins. M Immunofluorescence (IF) staining (original magnification: × 40; scale bar: 25 μm) examined IQGAP3 and IGF2BP2 expression in THP-1 cells

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Targeting IQGAP3 skews macrophages polarization towards M1 phenotype and promotes antitumor immune response

    doi: 10.1007/s00018-025-06044-6

    Figure Lengend Snippet: IQGAP3 reprogram macrophages through FYN-p-STAT1 axis. A Volcano plot showing differentially expressed genes revealed by RNA-seq analyses comparing sh-FOXM1 THP-1 cells and sh-NC THP-1 cells. Significantly upregulated (red) and downregulated (blue) genes are indicated, with thresholds set at |log₂ fold change|> 1 and adjusted p -value < 0.05. B Gene Set Enrichment Analysis (GSEA) of RNA-seq data revealed that a significant increase in the enrichment of the response to type I interferon, regulation of inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein in the IQGAP3 knockdown group. C The protein expression of p-STAT1 (Tyr701) and STAT1 were examined by WB in IQGAP3-knockdown THP-1 or BMDMs. D Representative histogram plot of flow cytometry and proportions of M1 macrophages (CD86 +) and M2 macrophages (CD206 +) in each indicated group. E The protein expression of FYN was examined by WB in sh-NC and sh-IQGAP3 THP-1-derived macrophages. F The protein expression of p-STAT1(Tyr701) in THP-1-derived macrophages for indicated conditions. G mRNA half-life (t 1/2 ) of FYN transcripts in sh-NC or sh-IQGAP3 THP-1-derived macrophages. H The number of the predicted binding sites for RNA binding proteins (RBPs) recorded on FYN mRNA 3′UTR region. I qPCR analysis of FYN mRNA levels in RIP assays with IgG and anti-IGFBP2 antibody in THP-1 cells. Relative enrichment is expressed by normalizing values to average IgG in each group. J mRNA half-life (t 1/2 ) of FYN transcripts in indicated groups. K The protein expression of p-STAT1(Tyr701) and FYN in THP-1 cells for indicated conditions. L Protein interaction was analyzed by co-immunoprecipitation (Co-IP) using an anti-IQGAP3 antibody in THP-1 cell lysates, followed by immunoblotting for the indicated proteins. M Immunofluorescence (IF) staining (original magnification: × 40; scale bar: 25 μm) examined IQGAP3 and IGF2BP2 expression in THP-1 cells

    Article Snippet: The immunoprecipitation magnetic beads (C0104, TargetMol) were washed and then incubated with anti-IQGAP3 (25930–1-AP, ProteinTech; 3 μg for 1–3 mg of total protein lysate) and IgG (30000–0-AP, ProteinTech; 3 μg for 1–3 mg of total protein lysate) at room temperature with gentle rotation for 30 min.

    Techniques: RNA Sequencing, Phospho-proteomics, Knockdown, Expressing, Flow Cytometry, Derivative Assay, Binding Assay, RNA Binding Assay, Immunoprecipitation, Co-Immunoprecipitation Assay, Western Blot, Immunofluorescence, Staining