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imscs  (ATCC)


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    Structured Review

    ATCC imscs
    Imscs, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imscs/AsC52telo%3B+Adipose+Tissue%3B+Human/pmc12940780-7-4-7
    Average 95 stars, based on 73 article reviews
    imscs - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism
    Article Snippet: .. Donor-matched iMSCs (ACS-7010; American Type Culture Collection, Manassas, VA, USA) and non-donor matched human BDMSCs (PC-500–012; American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) [+] 4.5 g/L glucose, L-glutamine and sodium pyruvate supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 1% non-essential amino acids in T-175 polystyrene tissue culture flasks. ..

    Modification:

    Article Title: Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism
    Article Snippet: .. Donor-matched iMSCs (ACS-7010; American Type Culture Collection, Manassas, VA, USA) and non-donor matched human BDMSCs (PC-500–012; American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) [+] 4.5 g/L glucose, L-glutamine and sodium pyruvate supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 1% non-essential amino acids in T-175 polystyrene tissue culture flasks. ..

    other:

    Article Title: Exosomes derived from mesenchymal stem cells primed with disease-condition-serum improved therapeutic efficacy in a mouse rheumatoid arthritis model via enhanced TGF-β1 production
    Article Snippet: iMSCs were purchased from ATCC (SCRC-4000) and maintained in MSC basal medium (PCS-500-030, ATCC) using an MSC growth kit for adipose and umbilical MSCs (PCS-500-040, ATCC) according to the manufacturer’s instructions.

    Expressing:

    Article Title: Characterization, Preconditioning, Safety, and Other Issues of MSC-Derived EVs and Secretome
    Article Snippet: AT-MSCs [ ] , hTERT-immortalized AT-MSCs (SCRC-4000TM cell line, purchased from ATCC ® ), adapted to suspension culture (S-hMSCs); S-hMSCs had comparable growth kinetics to primary and immortalized AT-MSCs; doubling time of app. 55 h; retained close to 90% of CD73 and CD105 expression levels, the CD90 receptor being downregulated during the adherent to suspension adaptation process, upregulation of the transcripts coding for CD44, CD46, and CD47 compared to the expression levels in primary and immortalized AT-MSCs (by RNA sequencing) , EVs isolated via ultracentrifugation; mean size 153 nm; expressing CD63, CD81, TSG101; functional studies are planned , Not yet performed. .. AT-MSCs [ ] , iMSCs purchased from ATCC ® (SCRC-4000 TM ); showed positive expression of CD29, CD44, CD73, CD90, CD105, absence of CD31, CD34, CD45, and HLA-DR; differentiation into adipocytes, osteocytes, and chondrocytes confirmed , Isolated using the Total Exosome Isolation Reagent (4478359, Invitrogen, Waltham, MA, USA) from iMSCs primed with serum derived from a non-human primate rheumatoid arthritis (RA) model; serum IL-4 and the proportion of Th2 (CD4+CD25+GATA3+) and M2 (CD11c-CD206+ of CD45+CD64+) cells were significantly increased compared to the control when sEVs-RA were administered to the collagen-induced arthritis model; sEVs-RA alleviated cartilage damage by significantly lowering the concentrations of pro-inflammatory cytokines (TNF-α, keratinocyte chemoattractant, IL-12p70) , Was not performed. .. AT-MSCs [ , ] , Expression of hTERT mRNA; nuclear hTERT immunoreaction; more than PD100, normal karyotype (with a clone with chromosomal aberrations after PD83), no signs of senescence by SA-bGal staining; retained expression of CD44, CD73, CD90, CD105, did not express CD3, CD14, CD19, CD34, CD45, HLA-DR; osteogenic and adipogenic differentiation; in vivo tumorigenicity was absent , Exo-spin Kit (EX01, Cell Guidance Sytems Ltd., Cambridge, UK) or ultracentrifugation followed by the PEG precipitation method applied for the enrichment of sEVs; remarkable number of sEVs with proper size (40–150 nm) and protein concentration produced, proper integrity, size distribution, and polydispersity index; positive for common exosomal markers, including CD63 and TSG101 , Was not performed.

    Isolation:

    Article Title: Characterization, Preconditioning, Safety, and Other Issues of MSC-Derived EVs and Secretome
    Article Snippet: AT-MSCs [ ] , hTERT-immortalized AT-MSCs (SCRC-4000TM cell line, purchased from ATCC ® ), adapted to suspension culture (S-hMSCs); S-hMSCs had comparable growth kinetics to primary and immortalized AT-MSCs; doubling time of app. 55 h; retained close to 90% of CD73 and CD105 expression levels, the CD90 receptor being downregulated during the adherent to suspension adaptation process, upregulation of the transcripts coding for CD44, CD46, and CD47 compared to the expression levels in primary and immortalized AT-MSCs (by RNA sequencing) , EVs isolated via ultracentrifugation; mean size 153 nm; expressing CD63, CD81, TSG101; functional studies are planned , Not yet performed. .. AT-MSCs [ ] , iMSCs purchased from ATCC ® (SCRC-4000 TM ); showed positive expression of CD29, CD44, CD73, CD90, CD105, absence of CD31, CD34, CD45, and HLA-DR; differentiation into adipocytes, osteocytes, and chondrocytes confirmed , Isolated using the Total Exosome Isolation Reagent (4478359, Invitrogen, Waltham, MA, USA) from iMSCs primed with serum derived from a non-human primate rheumatoid arthritis (RA) model; serum IL-4 and the proportion of Th2 (CD4+CD25+GATA3+) and M2 (CD11c-CD206+ of CD45+CD64+) cells were significantly increased compared to the control when sEVs-RA were administered to the collagen-induced arthritis model; sEVs-RA alleviated cartilage damage by significantly lowering the concentrations of pro-inflammatory cytokines (TNF-α, keratinocyte chemoattractant, IL-12p70) , Was not performed. .. AT-MSCs [ , ] , Expression of hTERT mRNA; nuclear hTERT immunoreaction; more than PD100, normal karyotype (with a clone with chromosomal aberrations after PD83), no signs of senescence by SA-bGal staining; retained expression of CD44, CD73, CD90, CD105, did not express CD3, CD14, CD19, CD34, CD45, HLA-DR; osteogenic and adipogenic differentiation; in vivo tumorigenicity was absent , Exo-spin Kit (EX01, Cell Guidance Sytems Ltd., Cambridge, UK) or ultracentrifugation followed by the PEG precipitation method applied for the enrichment of sEVs; remarkable number of sEVs with proper size (40–150 nm) and protein concentration produced, proper integrity, size distribution, and polydispersity index; positive for common exosomal markers, including CD63 and TSG101 , Was not performed.

    Derivative Assay:

    Article Title: Characterization, Preconditioning, Safety, and Other Issues of MSC-Derived EVs and Secretome
    Article Snippet: AT-MSCs [ ] , hTERT-immortalized AT-MSCs (SCRC-4000TM cell line, purchased from ATCC ® ), adapted to suspension culture (S-hMSCs); S-hMSCs had comparable growth kinetics to primary and immortalized AT-MSCs; doubling time of app. 55 h; retained close to 90% of CD73 and CD105 expression levels, the CD90 receptor being downregulated during the adherent to suspension adaptation process, upregulation of the transcripts coding for CD44, CD46, and CD47 compared to the expression levels in primary and immortalized AT-MSCs (by RNA sequencing) , EVs isolated via ultracentrifugation; mean size 153 nm; expressing CD63, CD81, TSG101; functional studies are planned , Not yet performed. .. AT-MSCs [ ] , iMSCs purchased from ATCC ® (SCRC-4000 TM ); showed positive expression of CD29, CD44, CD73, CD90, CD105, absence of CD31, CD34, CD45, and HLA-DR; differentiation into adipocytes, osteocytes, and chondrocytes confirmed , Isolated using the Total Exosome Isolation Reagent (4478359, Invitrogen, Waltham, MA, USA) from iMSCs primed with serum derived from a non-human primate rheumatoid arthritis (RA) model; serum IL-4 and the proportion of Th2 (CD4+CD25+GATA3+) and M2 (CD11c-CD206+ of CD45+CD64+) cells were significantly increased compared to the control when sEVs-RA were administered to the collagen-induced arthritis model; sEVs-RA alleviated cartilage damage by significantly lowering the concentrations of pro-inflammatory cytokines (TNF-α, keratinocyte chemoattractant, IL-12p70) , Was not performed. .. AT-MSCs [ , ] , Expression of hTERT mRNA; nuclear hTERT immunoreaction; more than PD100, normal karyotype (with a clone with chromosomal aberrations after PD83), no signs of senescence by SA-bGal staining; retained expression of CD44, CD73, CD90, CD105, did not express CD3, CD14, CD19, CD34, CD45, HLA-DR; osteogenic and adipogenic differentiation; in vivo tumorigenicity was absent , Exo-spin Kit (EX01, Cell Guidance Sytems Ltd., Cambridge, UK) or ultracentrifugation followed by the PEG precipitation method applied for the enrichment of sEVs; remarkable number of sEVs with proper size (40–150 nm) and protein concentration produced, proper integrity, size distribution, and polydispersity index; positive for common exosomal markers, including CD63 and TSG101 , Was not performed.

    Control:

    Article Title: Characterization, Preconditioning, Safety, and Other Issues of MSC-Derived EVs and Secretome
    Article Snippet: AT-MSCs [ ] , hTERT-immortalized AT-MSCs (SCRC-4000TM cell line, purchased from ATCC ® ), adapted to suspension culture (S-hMSCs); S-hMSCs had comparable growth kinetics to primary and immortalized AT-MSCs; doubling time of app. 55 h; retained close to 90% of CD73 and CD105 expression levels, the CD90 receptor being downregulated during the adherent to suspension adaptation process, upregulation of the transcripts coding for CD44, CD46, and CD47 compared to the expression levels in primary and immortalized AT-MSCs (by RNA sequencing) , EVs isolated via ultracentrifugation; mean size 153 nm; expressing CD63, CD81, TSG101; functional studies are planned , Not yet performed. .. AT-MSCs [ ] , iMSCs purchased from ATCC ® (SCRC-4000 TM ); showed positive expression of CD29, CD44, CD73, CD90, CD105, absence of CD31, CD34, CD45, and HLA-DR; differentiation into adipocytes, osteocytes, and chondrocytes confirmed , Isolated using the Total Exosome Isolation Reagent (4478359, Invitrogen, Waltham, MA, USA) from iMSCs primed with serum derived from a non-human primate rheumatoid arthritis (RA) model; serum IL-4 and the proportion of Th2 (CD4+CD25+GATA3+) and M2 (CD11c-CD206+ of CD45+CD64+) cells were significantly increased compared to the control when sEVs-RA were administered to the collagen-induced arthritis model; sEVs-RA alleviated cartilage damage by significantly lowering the concentrations of pro-inflammatory cytokines (TNF-α, keratinocyte chemoattractant, IL-12p70) , Was not performed. .. AT-MSCs [ , ] , Expression of hTERT mRNA; nuclear hTERT immunoreaction; more than PD100, normal karyotype (with a clone with chromosomal aberrations after PD83), no signs of senescence by SA-bGal staining; retained expression of CD44, CD73, CD90, CD105, did not express CD3, CD14, CD19, CD34, CD45, HLA-DR; osteogenic and adipogenic differentiation; in vivo tumorigenicity was absent , Exo-spin Kit (EX01, Cell Guidance Sytems Ltd., Cambridge, UK) or ultracentrifugation followed by the PEG precipitation method applied for the enrichment of sEVs; remarkable number of sEVs with proper size (40–150 nm) and protein concentration produced, proper integrity, size distribution, and polydispersity index; positive for common exosomal markers, including CD63 and TSG101 , Was not performed.



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    Image Search Results


    Effect of XF-iMSCs-secreted EVs in mouse primary cells. XF-iMSC-secreted EVs (XF-iEv) and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50). Data are presented as mean ± SD. + p < 0.05, ++ p < 0.01, +++p < 0.001 (Aspin-Welch's t -test), # p < 0.05; ## p < 0.01; ### p < 0.001 (Dunnett test, vs control group).

    Journal: Regenerative Therapy

    Article Title: Anti-inflammatory and immunomodulatory effects of human induced pluripotent stem cells-derived mesenchymal stem/stromal cells and their extracellular vesicles

    doi: 10.1016/j.reth.2026.101081

    Figure Lengend Snippet: Effect of XF-iMSCs-secreted EVs in mouse primary cells. XF-iMSC-secreted EVs (XF-iEv) and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50). Data are presented as mean ± SD. + p < 0.05, ++ p < 0.01, +++p < 0.001 (Aspin-Welch's t -test), # p < 0.05; ## p < 0.01; ### p < 0.001 (Dunnett test, vs control group).

    Article Snippet: XF-iMSC-secreted EVs (XF-iEv) and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50).

    Techniques: Derivative Assay, Purification, Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture, Control